Restriction map for
pF1KB7015
(Linear) (Six-base) MAPSORT of: KIBB7015.seq Check: 9483 from: 1 to: 2418
KIBB7015.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 273 688 1021 1750 2418
Size: 273 415 333 729 668
Fragments arranged by size:
729 668 415 333 273
AatII G_ACGT'C
Cuts at: 0 2030 2266 2418
Size: 2030 236 152
Fragments arranged by size:
2030 236 152
AccI GT'mk_AC
Cuts at: 0 2009 2418
Size: 2009 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 586 2140 2418
Size: 345 241 1554 278
Fragments arranged by size:
1554 345 278 241
AfeI AGC'GCT
Cuts at: 0 742 2418
Size: 742 1676
AflIII A'CryG_T
Cuts at: 0 1283 2418
Size: 1283 1135
AhdI GACnn_n'nnGTC
Cuts at: 0 1566 1782 2031 2418
Size: 1566 216 249 387
Fragments arranged by size:
1566 387 249 216
AleI CACnn'nnGTG
Cuts at: 0 362 433 2021 2418
Size: 362 71 1588 397
Fragments arranged by size:
1588 397 362 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 2418
Size: 631 1787
AlwNI CAG_nnn'CTG
Cuts at: 0 279 288 535 2418
Size: 279 9 247 1883
Fragments arranged by size:
1883 279 247 9
ApaI G_GGCC'C
Cuts at: 0 121 212 272 450 898 1345 1410 1649
Size: 121 91 60 178 448 447 65 239
Cuts at: 1649 2418
Size: 769
Fragments arranged by size:
769 448 447 239 178 121 91 65
60
ApaLI G'TGCA_C
Cuts at: 0 1231 1924 2418
Size: 1231 693 494
Fragments arranged by size:
1231 693 494
AvaI C'yCGr_G
Cuts at: 0 114 190 390 466 1369 1403 1716 1753
Size: 114 76 200 76 903 34 313 37
Cuts at: 1753 1805 1916 2313 2418
Size: 52 111 397 105
Fragments arranged by size:
903 397 313 200 114 111 105 76
76 52 37 34
BanI G'GyrC_C
Cuts at: 0 4 55 243 654 1627 1983 2292 2418
Size: 4 51 188 411 973 356 309 126
Fragments arranged by size:
973 411 356 309 188 126 51 4
BanII G_rGCy'C
Cuts at: 0 121 128 212 272 450 898 1222 1297
Size: 121 7 84 60 178 448 324 75
Cuts at: 1297 1345 1370 1410 1649 2077 2108 2410 2418
Size: 48 25 40 239 428 31 302 8
Fragments arranged by size:
448 428 324 302 239 178 121 84
75 60 48 40 31 25 8 7
BbeI G_GCGC'C
Cuts at: 0 8 59 1631 1987 2296 2418
Size: 8 51 1572 356 309 122
Fragments arranged by size:
1572 356 309 122 51 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 141 410 1954 2418
Size: 141 269 1544 464
Fragments arranged by size:
1544 464 269 141
BbvCI CC'TCA_GC
Cuts at: 0 422 1129 2418
Size: 422 707 1289
Fragments arranged by size:
1289 707 422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1360 1394 2418
Size: 1360 34 1024
Fragments arranged by size:
1360 1024 34
BclI T'GATC_A
Cuts at: 0 2174 2418
Size: 2174 244
BglI GCCn_nnn'nGGC
Cuts at: 0 49 276 499 902 1022 2418
Size: 49 227 223 403 120 1396
Fragments arranged by size:
1396 403 227 223 120 49
BglII A'GATC_T
Cuts at: 0 1238 2057 2418
Size: 1238 819 361
Fragments arranged by size:
1238 819 361
BlpI GC'TnA_GC
Cuts at: 0 175 2418
Size: 175 2243
Bme1580I G_kGCm'C
Cuts at: 0 121 212 248 272 450 659 898 1235
Size: 121 91 36 24 178 209 239 337
Cuts at: 1235 1345 1410 1649 1928 2418
Size: 110 65 239 279 490
Fragments arranged by size:
490 337 279 239 239 209 178 121
110 91 65 36 24
BmgBI CAC'GTC
Cuts at: 0 732 810 1923 2418
Size: 732 78 1113 495
Fragments arranged by size:
1113 732 495 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 987 2325 2418
Size: 987 1338 93
Fragments arranged by size:
1338 987 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1294 2322 2418
Size: 757 537 1028 96
Fragments arranged by size:
1028 757 537 96
Bpu10I CC'TnA_GC
Cuts at: 0 422 1129 2418
Size: 422 707 1289
Fragments arranged by size:
1289 707 422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 910 1240 2418
Size: 847 63 330 1178
Fragments arranged by size:
1178 847 330 63
BsaAI yAC'GTr
Cuts at: 0 870 1656 2418
Size: 870 786 762
Fragments arranged by size:
870 786 762
BsaBI GATnn'nnATC
Cuts at: 0 1894 2418
Size: 1894 524
BsaHI Gr'CG_yC
Cuts at: 0 5 56 998 1628 1984 2027 2263 2293
Size: 5 51 942 630 356 43 236 30
Cuts at: 2293 2418
Size: 125
Fragments arranged by size:
942 630 356 236 125 51 43 30
5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 292 322 366 396 682 712 1225 1255
Size: 292 30 44 30 286 30 513 30
Cuts at: 1255 1810 1840 2418
Size: 555 30 578
Fragments arranged by size:
578 555 513 292 286 44 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 1093 1096 1771 2116 2334 2418
Size: 51 1042 3 675 345 218 84
Fragments arranged by size:
1042 675 345 218 84 51 3
BseYI C'CCAG_C
Cuts at: 0 272 528 798 1623 2067 2332 2395 2418
Size: 272 256 270 825 444 265 63 23
Fragments arranged by size:
825 444 272 270 265 256 63 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1617 2139 2418
Size: 1617 522 279
Fragments arranged by size:
1617 522 279
BsiEI CG_ry'CG
Cuts at: 0 503 1962 2418
Size: 503 1459 456
Fragments arranged by size:
1459 503 456
BsiHKAI G_wGCw'C
Cuts at: 0 596 926 1235 1297 1370 1928 2108 2418
Size: 596 330 309 62 73 558 180 310
Fragments arranged by size:
596 558 330 310 309 180 73 62
BsmI GAATG_Cn'
Cuts at: 0 297 2418
Size: 297 2121
Bsp1286I G_dGCh'C
Cuts at: 0 121 128 212 248 272 450 596 659
Size: 121 7 84 36 24 178 146 63
Cuts at: 659 898 926 1222 1235 1297 1345 1370 1410
Size: 239 28 296 13 62 48 25 40
Cuts at: 1410 1649 1928 2077 2108 2410 2418
Size: 239 279 149 31 302 8
Fragments arranged by size:
302 296 279 239 239 178 149 146
121 84 63 62 48 40 36 31
28 25 24 13 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 273 688 1021 1628 1694 1750 2384 2418
Size: 273 415 333 607 66 56 634 34
Fragments arranged by size:
634 607 415 333 273 66 56 34
BsrBI CCG'CTC
Cuts at: 0 103 471 1254 2418
Size: 103 368 783 1164
Fragments arranged by size:
1164 783 368 103
BsrDI GCAATG_nn'
Cuts at: 0 1869 2418
Size: 1869 549
BsrFI r'CCGG_y
Cuts at: 0 52 326 498 752 1961 2418
Size: 52 274 172 254 1209 457
Fragments arranged by size:
1209 457 274 254 172 52
BsrGI T'GTAC_A
Cuts at: 0 830 2168 2418
Size: 830 1338 250
Fragments arranged by size:
1338 830 250
BssSI C'ACGA_G
Cuts at: 0 301 2418
Size: 301 2117
BstEII G'GTnAC_C
Cuts at: 0 1681 1872 2418
Size: 1681 191 546
Fragments arranged by size:
1681 546 191
BstYI r'GATC_y
Cuts at: 0 1238 2057 2418
Size: 1238 819 361
Fragments arranged by size:
1238 819 361
Bsu36I CC'TnA_GG
Cuts at: 0 1332 1988 2346 2418
Size: 1332 656 358 72
Fragments arranged by size:
1332 656 358 72
BtgI C'CryG_G
Cuts at: 0 38 203 585 666 2418
Size: 38 165 382 81 1752
Fragments arranged by size:
1752 382 165 81 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1302 2418
Size: 1302 1116
BtsI GCAGTG_nn'
Cuts at: 0 634 819 1116 2406 2418
Size: 634 185 297 1290 12
Fragments arranged by size:
1290 634 297 185 12
EaeI y'GGCC_r
Cuts at: 0 35 41 50 129 491 500 779 1352
Size: 35 6 9 79 362 9 279 573
Cuts at: 1352 1449 1673 1959 2319 2418
Size: 97 224 286 360 99
Fragments arranged by size:
573 362 360 286 279 224 99 97
79 35 9 9 6
EagI C'GGCC_G
Cuts at: 0 500 1959 2418
Size: 500 1459 459
Fragments arranged by size:
1459 500 459
EarI CTCTTCn'nnn_
Cuts at: 0 2113 2418
Size: 2113 305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1480 2418
Size: 1480 938
EcoICRI GAG'CTC
Cuts at: 0 1295 1368 2106 2418
Size: 1295 73 738 312
Fragments arranged by size:
1295 738 312 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 586 757 1294 2140 2322 2418
Size: 345 241 171 537 846 182 96
Fragments arranged by size:
846 537 345 241 182 171 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1435 2208 2418
Size: 1435 773 210
Fragments arranged by size:
1435 773 210
EcoO109I rG'GnC_Cy
Cuts at: 0 208 268 269 314 446 447 1341 1342
Size: 208 60 1 45 132 1 894 1
Cuts at: 1342 1551 1646 1776 1848 2043 2238 2418
Size: 209 95 130 72 195 195 180
Fragments arranged by size:
894 209 208 195 195 180 132 130
95 72 60 45 1 1 1
FspI TGC'GCA
Cuts at: 0 1195 1315 2418
Size: 1195 120 1103
Fragments arranged by size:
1195 1103 120
FspAI rTGC'GCAy
Cuts at: 0 1315 2418
Size: 1315 1103
HaeII r_GCGC'y
Cuts at: 0 8 59 744 941 1631 1987 2296 2418
Size: 8 51 685 197 690 356 309 122
Fragments arranged by size:
690 685 356 309 197 122 51 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 366 370 398 402 1552 1584 1768 1800
Size: 366 4 28 4 1150 32 184 32
Cuts at: 1800 2017 2049 2418
Size: 217 32 369
Fragments arranged by size:
1150 369 366 217 184 32 32 32
28 4 4
KasI G'GCGC_C
Cuts at: 0 4 55 1627 1983 2292 2418
Size: 4 51 1572 356 309 126
Fragments arranged by size:
1572 356 309 126 51 4
MluI A'CGCG_T
Cuts at: 0 1283 2418
Size: 1283 1135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1304 1322 1538 2418
Size: 1304 18 216 880
Fragments arranged by size:
1304 880 216 18
MscI TGG'CCA
Cuts at: 0 43 1354 2321 2418
Size: 43 1311 967 97
Fragments arranged by size:
1311 967 97 43
MslI CAynn'nnrTG
Cuts at: 0 362 433 1162 2021 2095 2176 2418
Size: 362 71 729 859 74 81 242
Fragments arranged by size:
859 729 362 242 81 74 71
MspA1I CmG'CkG
Cuts at: 0 158 276 323 333 524 535 587 668
Size: 158 118 47 10 191 11 52 81
Cuts at: 668 2229 2418
Size: 1561 189
Fragments arranged by size:
1561 191 189 158 118 81 52 47
11 10
NaeI GCC'GGC
Cuts at: 0 54 328 500 1963 2418
Size: 54 274 172 1463 455
Fragments arranged by size:
1463 455 274 172 54
NarI GG'CG_CC
Cuts at: 0 5 56 1628 1984 2293 2418
Size: 5 51 1572 356 309 125
Fragments arranged by size:
1572 356 309 125 51 5
NcoI C'CATG_G
Cuts at: 0 203 2418
Size: 203 2215
NgoMIV G'CCGG_C
Cuts at: 0 52 326 498 1961 2418
Size: 52 274 172 1463 457
Fragments arranged by size:
1463 457 274 172 52
NspI r_CATG'y
Cuts at: 0 2197 2418
Size: 2197 221
PasI CC'CwG_GG
Cuts at: 0 1419 2418
Size: 1419 999
PflMI CCAn_nnn'nTGG
Cuts at: 0 218 535 560 2136 2418
Size: 218 317 25 1576 282
Fragments arranged by size:
1576 317 282 218 25
PmlI CAC'GTG
Cuts at: 0 870 2418
Size: 870 1548
PpuMI rG'GwC_Cy
Cuts at: 0 1551 1776 1848 2043 2238 2418
Size: 1551 225 72 195 195 180
Fragments arranged by size:
1551 225 195 195 180 72
PshAI GACnn'nnGTC
Cuts at: 0 1266 2418
Size: 1266 1152
PspOMI G'GGCC_C
Cuts at: 0 117 208 268 446 894 1341 1406 1645
Size: 117 91 60 178 448 447 65 239
Cuts at: 1645 2418
Size: 773
Fragments arranged by size:
773 448 447 239 178 117 91 65
60
PspXI vC'TCGA_Gb
Cuts at: 0 1369 2418
Size: 1369 1049
PstI C_TGCA'G
Cuts at: 0 284 767 2418
Size: 284 483 1651
Fragments arranged by size:
1651 483 284
PvuII CAG'CTG
Cuts at: 0 323 2229 2418
Size: 323 1906 189
Fragments arranged by size:
1906 323 189
RsrII CG'GwC_CG
Cuts at: 0 663 2418
Size: 663 1755
SacI G_AGCT'C
Cuts at: 0 1297 1370 2108 2418
Size: 1297 73 738 310
Fragments arranged by size:
1297 738 310 73
SacII CC_GC'GG
Cuts at: 0 588 669 2418
Size: 588 81 1749
Fragments arranged by size:
1749 588 81
SapI GCTCTTCn'nnn_
Cuts at: 0 2113 2418
Size: 2113 305
SbfI CC_TGCA'GG
Cuts at: 0 767 2418
Size: 767 1651
ScaI AGT'ACT
Cuts at: 0 1819 2308 2418
Size: 1819 489 110
Fragments arranged by size:
1819 489 110
SexAI A'CCwGG_T
Cuts at: 0 1562 1778 1796 2418
Size: 1562 216 18 622
Fragments arranged by size:
1562 622 216 18
SfcI C'TryA_G
Cuts at: 0 280 318 763 2418
Size: 280 38 445 1655
Fragments arranged by size:
1655 445 280 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 49 499 2418
Size: 49 450 1919
Fragments arranged by size:
1919 450 49
SfoI GGC'GCC
Cuts at: 0 6 57 1629 1985 2294 2418
Size: 6 51 1572 356 309 124
Fragments arranged by size:
1572 356 309 124 51 6
SmaI CCC'GGG
Cuts at: 0 116 192 1718 1807 1918 2315 2418
Size: 116 76 1526 89 111 397 103
Fragments arranged by size:
1526 397 116 111 103 89 76
SmlI C'TyrA_G
Cuts at: 0 862 925 1255 1369 2418
Size: 862 63 330 114 1049
Fragments arranged by size:
1049 862 330 114 63
SphI G_CATG'C
Cuts at: 0 2197 2418
Size: 2197 221
StuI AGG'CCT
Cuts at: 0 1214 1993 2418
Size: 1214 779 425
Fragments arranged by size:
1214 779 425
StyI C'CwwG_G
Cuts at: 0 71 203 1431 1676 2418
Size: 71 132 1228 245 742
Fragments arranged by size:
1228 742 245 132 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1891 2018 2409 2418
Size: 1891 127 391 9
Fragments arranged by size:
1891 391 127 9
TatI w'GTAC_w
Cuts at: 0 830 1007 1817 2168 2306 2418
Size: 830 177 810 351 138 112
Fragments arranged by size:
830 810 351 177 138 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 1225 1257 2418
Size: 292 32 901 32 1161
Fragments arranged by size:
1161 901 292 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2005 2248 2418
Size: 2005 243 170
Fragments arranged by size:
2005 243 170
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1212 1305 2418
Size: 1212 93 1113
Fragments arranged by size:
1212 1113 93
XhoI C'TCGA_G
Cuts at: 0 1369 2418
Size: 1369 1049
XmaI C'CCGG_G
Cuts at: 0 114 190 1716 1805 1916 2313 2418
Size: 114 76 1526 89 111 397 105
Fragments arranged by size:
1526 397 114 111 105 89 76
ZraI GAC'GTC
Cuts at: 0 2028 2264 2418
Size: 2028 236 154
Fragments arranged by size:
2028 236 154
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PmlI PpuMI PshAI
PspOMI PspXI PstI PvuII RsrII SacI SacII SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BfrBI BmrI BmtI
BsaI BsaWI BsiWI BsmBI BspEI BspHI BssHII BstAPI
BstBI BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI
MfeI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PpiI PsiI PsrI PvuI SalI SanDI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI
XmnI