Restriction map for
pF1KB7016
(Linear) (Six-base) MAPSORT of: KIBB7016.seq Check: 8725 from: 1 to: 2505
KIBB7016.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 691 1964 2505
Size: 691 1273 541
Fragments arranged by size:
1273 691 541
Acc65I G'GTAC_C
Cuts at: 0 1828 2505
Size: 1828 677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2044 2505
Size: 2044 461
AfeI AGC'GCT
Cuts at: 0 94 2368 2505
Size: 94 2274 137
Fragments arranged by size:
2274 137 94
AhdI GACnn_n'nnGTC
Cuts at: 0 2346 2505
Size: 2346 159
AleI CACnn'nnGTG
Cuts at: 0 340 2071 2505
Size: 340 1731 434
Fragments arranged by size:
1731 434 340
AlwNI CAG_nnn'CTG
Cuts at: 0 494 1258 1606 2478 2505
Size: 494 764 348 872 27
Fragments arranged by size:
872 764 494 348 27
ApaI G_GGCC'C
Cuts at: 0 64 281 1125 1292 1714 2505
Size: 64 217 844 167 422 791
Fragments arranged by size:
844 791 422 217 167 64
AvaI C'yCGr_G
Cuts at: 0 57 63 220 306 599 1851 2505
Size: 57 6 157 86 293 1252 654
Fragments arranged by size:
1252 654 293 157 86 57 6
BamHI G'GATC_C
Cuts at: 0 1140 2505
Size: 1140 1365
BanI G'GyrC_C
Cuts at: 0 163 334 1452 1627 1828 2206 2505
Size: 163 171 1118 175 201 378 299
Fragments arranged by size:
1118 378 299 201 175 171 163
BanII G_rGCy'C
Cuts at: 0 64 76 233 281 1056 1125 1284 1292
Size: 64 12 157 48 775 69 159 8
Cuts at: 1292 1309 1388 1692 1714 2435 2505
Size: 17 79 304 22 721 70
Fragments arranged by size:
775 721 304 159 157 79 70 69
64 48 22 17 12 8
BbeI G_GCGC'C
Cuts at: 0 167 2505
Size: 167 2338
BbsI GAAGACnn'nnnn_
Cuts at: 0 1506 1515 2505
Size: 1506 9 990
Fragments arranged by size:
1506 990 9
BbvCI CC'TCA_GC
Cuts at: 0 132 1760 1833 2044 2505
Size: 132 1628 73 211 461
Fragments arranged by size:
1628 461 211 132 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 20 54 2430 2464 2505
Size: 20 34 2376 34 41
Fragments arranged by size:
2376 41 34 34 20
BglI GCCn_nnn'nGGC
Cuts at: 0 13 109 1051 2505
Size: 13 96 942 1454
Fragments arranged by size:
1454 942 96 13
Bme1580I G_kGCm'C
Cuts at: 0 64 281 1125 1292 1632 1714 2235 2505
Size: 64 217 844 167 340 82 521 270
Fragments arranged by size:
844 521 340 270 217 167 82 64
BmgBI CAC'GTC
Cuts at: 0 2343 2505
Size: 2343 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 593 2505
Size: 593 1912
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1848 2505
Size: 1848 657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 205 1003 1078 1828 2224 2505
Size: 196 9 798 75 750 396 281
Fragments arranged by size:
798 750 396 281 196 75 9
Bpu10I CC'TnA_GC
Cuts at: 0 132 273 1294 1416 1760 1800 1833 2044
Size: 132 141 1021 122 344 40 33 211
Cuts at: 2044 2505
Size: 461
Fragments arranged by size:
1021 461 344 211 141 132 122 40
33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1359 2505
Size: 1111 248 1146
Fragments arranged by size:
1146 1111 248
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 1711 2505
Size: 517 1194 794
Fragments arranged by size:
1194 794 517
BsaBI GATnn'nnATC
Cuts at: 0 892 2505
Size: 892 1613
BsaHI Gr'CG_yC
Cuts at: 0 142 164 434 2505
Size: 142 22 270 2071
Fragments arranged by size:
2071 270 142 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 2236 2266 2505
Size: 502 30 1704 30 239
Fragments arranged by size:
1704 502 239 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1678 1681 1999 2044 2505
Size: 1678 3 318 45 461
Fragments arranged by size:
1678 461 318 45 3
BseYI C'CCAG_C
Cuts at: 0 480 2505
Size: 480 2025
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 441 1099 1244 1354 1400 2078 2365
Size: 222 219 658 145 110 46 678 287
Cuts at: 2365 2505
Size: 140
Fragments arranged by size:
678 658 287 222 219 145 140 110
46
BsiEI CG_ry'CG
Cuts at: 0 44 113 2505
Size: 44 69 2392
Fragments arranged by size:
2392 69 44
BsiHKAI G_wGCw'C
Cuts at: 0 191 508 820 1031 2015 2505
Size: 191 317 312 211 984 490
Fragments arranged by size:
984 490 317 312 211 191
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1663 2371 2505
Size: 1663 708 134
Fragments arranged by size:
1663 708 134
Bsp1286I G_dGCh'C
Cuts at: 0 64 76 191 233 281 508 820 1031
Size: 64 12 115 42 48 227 312 211
Cuts at: 1031 1056 1125 1284 1292 1309 1388 1632 1692
Size: 25 69 159 8 17 79 244 60
Cuts at: 1692 1714 2015 2235 2435 2505
Size: 22 301 220 200 70
Fragments arranged by size:
312 301 244 227 220 211 200 159
115 79 70 69 64 60 48 42
25 22 17 12 8
BspHI T'CATG_A
Cuts at: 0 905 2505
Size: 905 1600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 691 1964 2505
Size: 691 1273 541
Fragments arranged by size:
1273 691 541
BsrBI CCG'CTC
Cuts at: 0 987 1415 1436 2505
Size: 987 428 21 1069
Fragments arranged by size:
1069 987 428 21
BsrDI GCAATG_nn'
Cuts at: 0 805 2505
Size: 805 1700
BsrFI r'CCGG_y
Cuts at: 0 1591 1959 2505
Size: 1591 368 546
Fragments arranged by size:
1591 546 368
BssSI C'ACGA_G
Cuts at: 0 1457 2505
Size: 1457 1048
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1035 2505
Size: 1035 1470
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2203 2505
Size: 2203 302
BstYI r'GATC_y
Cuts at: 0 1010 1140 2214 2505
Size: 1010 130 1074 291
Fragments arranged by size:
1074 1010 291 130
Bsu36I CC'TnA_GG
Cuts at: 0 1823 2505
Size: 1823 682
BtgI C'CryG_G
Cuts at: 0 11 137 152 281 338 1145 1265 1325
Size: 11 126 15 129 57 807 120 60
Cuts at: 1325 2505
Size: 1180
Fragments arranged by size:
1180 807 129 126 120 60 57 15
11
BtsI GCAGTG_nn'
Cuts at: 0 189 749 2505
Size: 189 560 1756
Fragments arranged by size:
1756 560 189
DraIII CAC_nnn'GTG
Cuts at: 0 1786 2505
Size: 1786 719
EaeI y'GGCC_r
Cuts at: 0 41 110 890 1593 1649 2078 2505
Size: 41 69 780 703 56 429 427
Fragments arranged by size:
780 703 429 427 69 56 41
EagI C'GGCC_G
Cuts at: 0 41 110 2505
Size: 41 69 2395
Fragments arranged by size:
2395 69 41
EarI CTCTTCn'nnn_
Cuts at: 0 808 2505
Size: 808 1697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 832 2505
Size: 832 1673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 205 1003 1078 1828 2044 2224 2505
Size: 196 9 798 75 750 216 180 281
Fragments arranged by size:
798 750 281 216 196 180 75 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2026 2505
Size: 2026 479
EcoO109I rG'GnC_Cy
Cuts at: 0 277 1122 1179 1255 1288 1289 1710 1711
Size: 277 845 57 76 33 1 421 1
Cuts at: 1711 1750 1765 2505
Size: 39 15 740
Fragments arranged by size:
845 740 421 277 76 57 39 33
15 1 1
FspI TGC'GCA
Cuts at: 0 1447 2505
Size: 1447 1058
HaeII r_GCGC'y
Cuts at: 0 96 167 175 213 786 981 1577 2370
Size: 96 71 8 38 573 195 596 793
Cuts at: 2370 2505
Size: 135
Fragments arranged by size:
793 596 573 195 135 96 71 38
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1125 1157 1357 1389 2236 2268 2332 2364
Size: 1125 32 200 32 847 32 64 32
Cuts at: 2364 2505
Size: 141
Fragments arranged by size:
1125 847 200 141 64 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2324 2505
Size: 2324 181
HpaI GTT'AAC
Cuts at: 0 2324 2505
Size: 2324 181
KasI G'GCGC_C
Cuts at: 0 163 2505
Size: 163 2342
KpnI G_GTAC'C
Cuts at: 0 1832 2505
Size: 1832 673
MscI TGG'CCA
Cuts at: 0 892 1651 2080 2505
Size: 892 759 429 425
Fragments arranged by size:
892 759 429 425
MslI CAynn'nnrTG
Cuts at: 0 340 638 1330 2071 2505
Size: 340 298 692 741 434
Fragments arranged by size:
741 692 434 340 298
MspA1I CmG'CkG
Cuts at: 0 13 34 79 139 154 283 546 1113
Size: 13 21 45 60 15 129 263 567
Cuts at: 1113 1495 1582 2103 2166 2505
Size: 382 87 521 63 339
Fragments arranged by size:
567 521 382 339 263 129 87 63
60 45 21 15 13
NaeI GCC'GGC
Cuts at: 0 1961 2505
Size: 1961 544
NarI GG'CG_CC
Cuts at: 0 164 2505
Size: 164 2341
NcoI C'CATG_G
Cuts at: 0 1265 1325 2505
Size: 1265 60 1180
Fragments arranged by size:
1265 1180 60
NgoMIV G'CCGG_C
Cuts at: 0 1959 2505
Size: 1959 546
NspI r_CATG'y
Cuts at: 0 802 2304 2505
Size: 802 1502 201
Fragments arranged by size:
1502 802 201
PasI CC'CwG_GG
Cuts at: 0 1285 2505
Size: 1285 1220
PflMI CCAn_nnn'nTGG
Cuts at: 0 1326 1846 2292 2505
Size: 1326 520 446 213
Fragments arranged by size:
1326 520 446 213
PfoI T'CCnGG_A
Cuts at: 0 2240 2505
Size: 2240 265
PpuMI rG'GwC_Cy
Cuts at: 0 1179 1750 1765 2505
Size: 1179 571 15 740
Fragments arranged by size:
1179 740 571 15
PspOMI G'GGCC_C
Cuts at: 0 60 277 1121 1288 1710 2505
Size: 60 217 844 167 422 795
Fragments arranged by size:
844 795 422 217 167 60
PstI C_TGCA'G
Cuts at: 0 902 1587 1617 1970 2366 2505
Size: 902 685 30 353 396 139
Fragments arranged by size:
902 685 396 353 139 30
PvuII CAG'CTG
Cuts at: 0 1113 1495 2166 2505
Size: 1113 382 671 339
Fragments arranged by size:
1113 671 382 339
SacII CC_GC'GG
Cuts at: 0 14 140 155 284 2505
Size: 14 126 15 129 2221
Fragments arranged by size:
2221 129 126 15 14
SanDI GG'GwC_CC
Cuts at: 0 1765 2505
Size: 1765 740
SapI GCTCTTCn'nnn_
Cuts at: 0 808 2505
Size: 808 1697
SbfI CC_TGCA'GG
Cuts at: 0 1617 1970 2505
Size: 1617 353 535
Fragments arranged by size:
1617 535 353
SfcI C'TryA_G
Cuts at: 0 261 898 1583 1613 1966 2262 2362 2505
Size: 261 637 685 30 353 296 100 143
Fragments arranged by size:
685 637 353 296 261 143 100 30
SfoI GGC'GCC
Cuts at: 0 165 2505
Size: 165 2340
SmaI CCC'GGG
Cuts at: 0 59 65 1853 2505
Size: 59 6 1788 652
Fragments arranged by size:
1788 652 59 6
SmlI C'TyrA_G
Cuts at: 0 1126 1374 2505
Size: 1126 248 1131
Fragments arranged by size:
1131 1126 248
SrfI GCCC'GGGC
Cuts at: 0 59 65 2505
Size: 59 6 2440
Fragments arranged by size:
2440 59 6
StuI AGG'CCT
Cuts at: 0 1210 2395 2505
Size: 1210 1185 110
Fragments arranged by size:
1210 1185 110
StyI C'CwwG_G
Cuts at: 0 560 786 821 1265 1325 2505
Size: 560 226 35 444 60 1180
Fragments arranged by size:
1180 560 444 226 60 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1278 2505
Size: 1278 1227
TatI w'GTAC_w
Cuts at: 0 554 2118 2505
Size: 554 1564 387
Fragments arranged by size:
1564 554 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 1185 1217 2505
Size: 502 32 651 32 1288
Fragments arranged by size:
1288 651 502 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 495 1803 1985 2187 2479 2505
Size: 384 111 1308 182 202 292 26
Fragments arranged by size:
1308 384 292 202 182 111 26
XmaI C'CCGG_G
Cuts at: 0 57 63 1851 2505
Size: 57 6 1788 654
Fragments arranged by size:
1788 654 57 6
XmnI GAAnn'nnTTC
Cuts at: 0 397 2505
Size: 397 2108
Enzymes that do cut:
AarI Acc65I AcuI AfeI AhdI AleI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII HpaI KasI KpnI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacII SanDI SapI
SbfI SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglII BlpI BmtI BsaAI BsaWI BsiWI
BsmI BspEI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI
FseI FspAI HindIII MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI ZraI