Restriction map for
pF1KB7018
(Linear) (Six-base) MAPSORT of: KIBB7018.seq Check: 201 from: 1 to: 2916
KIBB7018.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2682 2916
Size: 2682 234
AatII G_ACGT'C
Cuts at: 0 1196 2543 2916
Size: 1196 1347 373
Fragments arranged by size:
1347 1196 373
AccI GT'mk_AC
Cuts at: 0 2522 2840 2916
Size: 2522 318 76
Fragments arranged by size:
2522 318 76
AclI AA'CG_TT
Cuts at: 0 820 2916
Size: 820 2096
AflII C'TTAA_G
Cuts at: 0 306 2916
Size: 306 2610
AflIII A'CryG_T
Cuts at: 0 284 2525 2916
Size: 284 2241 391
Fragments arranged by size:
2241 391 284
AhdI GACnn_n'nnGTC
Cuts at: 0 2544 2916
Size: 2544 372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 598 2916
Size: 598 2318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1898 1930 2916
Size: 1898 32 986
Fragments arranged by size:
1898 986 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1336 2086 2895 2916
Size: 1336 750 809 21
Fragments arranged by size:
1336 809 750 21
ApaI G_GGCC'C
Cuts at: 0 1982 2916
Size: 1982 934
ApaLI G'TGCA_C
Cuts at: 0 288 696 2902 2916
Size: 288 408 2206 14
Fragments arranged by size:
2206 408 288 14
ApoI r'AATT_y
Cuts at: 0 595 682 2025 2916
Size: 595 87 1343 891
Fragments arranged by size:
1343 891 595 87
AseI AT'TA_AT
Cuts at: 0 938 2916
Size: 938 1978
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1107 1140 2916
Size: 1107 33 1776
Fragments arranged by size:
1776 1107 33
BanI G'GyrC_C
Cuts at: 0 16 449 1150 2496 2916
Size: 16 433 701 1346 420
Fragments arranged by size:
1346 701 433 420 16
BanII G_rGCy'C
Cuts at: 0 1307 1982 2916
Size: 1307 675 934
Fragments arranged by size:
1307 934 675
BbeI G_GCGC'C
Cuts at: 0 20 2916
Size: 20 2896
BbsI GAAGACnn'nnnn_
Cuts at: 0 389 2114 2294 2704 2916
Size: 389 1725 180 410 212
Fragments arranged by size:
1725 410 389 212 180
BclI T'GATC_A
Cuts at: 0 512 1722 2633 2916
Size: 512 1210 911 283
Fragments arranged by size:
1210 911 512 283
BglII A'GATC_T
Cuts at: 0 2206 2916
Size: 2206 710
BlpI GC'TnA_GC
Cuts at: 0 2296 2656 2916
Size: 2296 360 260
Fragments arranged by size:
2296 360 260
Bme1580I G_kGCm'C
Cuts at: 0 292 700 1875 1982 2906 2916
Size: 292 408 1175 107 924 10
Fragments arranged by size:
1175 924 408 292 107 10
BmgBI CAC'GTC
Cuts at: 0 695 2916
Size: 695 2221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 26 2592 2916
Size: 26 2566 324
Fragments arranged by size:
2566 324 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1799 2186 2916
Size: 875 924 387 730
Fragments arranged by size:
924 875 730 387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 498 1846 2916
Size: 427 71 1348 1070
Fragments arranged by size:
1348 1070 427 71
BsaAI yAC'GTr
Cuts at: 0 287 1119 2916
Size: 287 832 1797
Fragments arranged by size:
1797 832 287
BsaBI GATnn'nnATC
Cuts at: 0 2714 2916
Size: 2714 202
BsaHI Gr'CG_yC
Cuts at: 0 17 1193 2540 2916
Size: 17 1176 1347 376
Fragments arranged by size:
1347 1176 376 17
BsaWI w'CCGG_w
Cuts at: 0 2648 2916
Size: 2648 268
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 974 1004 2916
Size: 562 30 382 30 1912
Fragments arranged by size:
1912 562 382 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1684 2874 2916
Size: 1684 1190 42
Fragments arranged by size:
1684 1190 42
BseYI C'CCAG_C
Cuts at: 0 2705 2888 2916
Size: 2705 183 28
Fragments arranged by size:
2705 183 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1396 1950 2072 2916
Size: 1396 554 122 844
Fragments arranged by size:
1396 844 554 122
BsiEI CG_ry'CG
Cuts at: 0 2612 2916
Size: 2612 304
BsiHKAI G_wGCw'C
Cuts at: 0 292 700 2782 2906 2916
Size: 292 408 2082 124 10
Fragments arranged by size:
2082 408 292 124 10
BsmI GAATG_Cn'
Cuts at: 0 1015 1444 2109 2609 2916
Size: 1015 429 665 500 307
Fragments arranged by size:
1015 665 500 429 307
BsmBI CGTCTCn'nnnn_
Cuts at: 0 107 2916
Size: 107 2809
Bsp1286I G_dGCh'C
Cuts at: 0 292 700 1307 1875 1982 2782 2906 2916
Size: 292 408 607 568 107 800 124 10
Fragments arranged by size:
800 607 568 408 292 124 107 10
BspEI T'CCGG_A
Cuts at: 0 2648 2916
Size: 2648 268
BspHI T'CATG_A
Cuts at: 0 509 2916
Size: 509 2407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2682 2916
Size: 2682 234
BsrBI CCG'CTC
Cuts at: 0 365 2916
Size: 365 2551
BsrFI r'CCGG_y
Cuts at: 0 274 2607 2916
Size: 274 2333 309
Fragments arranged by size:
2333 309 274
BssSI C'ACGA_G
Cuts at: 0 1261 2916
Size: 1261 1655
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1595 2086 2916
Size: 1595 491 830
Fragments arranged by size:
1595 830 491
BstYI r'GATC_y
Cuts at: 0 206 336 1043 2206 2715 2916
Size: 206 130 707 1163 509 201
Fragments arranged by size:
1163 707 509 206 201 130
BstZ17I GTA'TAC
Cuts at: 0 2523 2916
Size: 2523 393
BtgI C'CryG_G
Cuts at: 0 2834 2916
Size: 2834 82
BtsI GCAGTG_nn'
Cuts at: 0 1045 2916
Size: 1045 1871
DraI TTT'AAA
Cuts at: 0 1524 2916
Size: 1524 1392
DrdI GACnn_nn'nnGTC
Cuts at: 0 139 397 2916
Size: 139 258 2519
Fragments arranged by size:
2519 258 139
EaeI y'GGCC_r
Cuts at: 0 1835 2916
Size: 1835 1081
EarI CTCTTCn'nnn_
Cuts at: 0 75 470 2916
Size: 75 395 2446
Fragments arranged by size:
2446 395 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 875 1799 2186 2916
Size: 875 924 387 730
Fragments arranged by size:
924 875 730 387
EcoO109I rG'GnC_Cy
Cuts at: 0 1978 2916
Size: 1978 938
EcoRI G'AATT_C
Cuts at: 0 682 2916
Size: 682 2234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2107 2661 2916
Size: 2107 554 255
Fragments arranged by size:
2107 554 255
HaeII r_GCGC'y
Cuts at: 0 11 20 2916
Size: 11 9 2896
Fragments arranged by size:
2896 11 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 2530 2562 2769 2801 2916
Size: 562 32 1936 32 207 32 115
Fragments arranged by size:
1936 562 207 115 32 32 32
HincII GTy'rAC
Cuts at: 0 360 824 2916
Size: 360 464 2092
Fragments arranged by size:
2092 464 360
HindIII A'AGCT_T
Cuts at: 0 346 2916
Size: 346 2570
KasI G'GCGC_C
Cuts at: 0 16 2916
Size: 16 2900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 2377 2822 2916
Size: 569 1808 445 94
Fragments arranged by size:
1808 569 445 94
MslI CAynn'nnrTG
Cuts at: 0 322 791 1603 2155 2909 2916
Size: 322 469 812 552 754 7
Fragments arranged by size:
812 754 552 469 322 7
MspA1I CmG'CkG
Cuts at: 0 404 2916
Size: 404 2512
NarI GG'CG_CC
Cuts at: 0 17 2916
Size: 17 2899
NspI r_CATG'y
Cuts at: 0 1275 2916
Size: 1275 1641
PacI TTA_AT'TAA
Cuts at: 0 1823 2916
Size: 1823 1093
PasI CC'CwG_GG
Cuts at: 0 1770 2916
Size: 1770 1146
PflMI CCAn_nnn'nTGG
Cuts at: 0 1284 1405 2916
Size: 1284 121 1511
Fragments arranged by size:
1511 1284 121
PfoI T'CCnGG_A
Cuts at: 0 120 209 1329 2598 2916
Size: 120 89 1120 1269 318
Fragments arranged by size:
1269 1120 318 120 89
PmlI CAC'GTG
Cuts at: 0 287 2916
Size: 287 2629
PsiI TTA'TAA
Cuts at: 0 2096 2916
Size: 2096 820
PspOMI G'GGCC_C
Cuts at: 0 1978 2916
Size: 1978 938
PstI C_TGCA'G
Cuts at: 0 2131 2815 2916
Size: 2131 684 101
Fragments arranged by size:
2131 684 101
SapI GCTCTTCn'nnn_
Cuts at: 0 75 2916
Size: 75 2841
ScaI AGT'ACT
Cuts at: 0 2139 2916
Size: 2139 777
SexAI A'CCwGG_T
Cuts at: 0 2309 2916
Size: 2309 607
SfcI C'TryA_G
Cuts at: 0 763 962 1430 2127 2811 2916
Size: 763 199 468 697 684 105
Fragments arranged by size:
763 697 684 468 199 105
SfoI GGC'GCC
Cuts at: 0 18 2916
Size: 18 2898
SmlI C'TyrA_G
Cuts at: 0 306 442 477 1861 2916
Size: 306 136 35 1384 1055
Fragments arranged by size:
1384 1055 306 136 35
SnaBI TAC'GTA
Cuts at: 0 1119 2916
Size: 1119 1797
SphI G_CATG'C
Cuts at: 0 1275 2916
Size: 1275 1641
SspI AAT'ATT
Cuts at: 0 1627 1944 2002 2916
Size: 1627 317 58 914
Fragments arranged by size:
1627 914 317 58
StyI C'CwwG_G
Cuts at: 0 470 497 911 1926 2189 2916
Size: 470 27 414 1015 263 727
Fragments arranged by size:
1015 727 470 414 263 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1762 2916
Size: 1762 1154
TatI w'GTAC_w
Cuts at: 0 725 1359 1650 2137 2147 2916
Size: 725 634 291 487 10 769
Fragments arranged by size:
769 725 634 487 291 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 98 130 1400 1432 2916
Size: 98 32 1270 32 1484
Fragments arranged by size:
1484 1270 98 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2911 2916
Size: 2911 5
ZraI GAC'GTC
Cuts at: 0 1194 2541 2916
Size: 1194 1347 375
Fragments arranged by size:
1347 1194 375
Enzymes that do cut:
AarI AatII AccI AclI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI BaeI BanI
BanII BbeI BbsI BclI BglII BlpI Bme1580I BmgBI
BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstYI
BstZ17I BtgI BtsI DraI DrdI EaeI EarI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI
MmeI MslI MspA1I NarI NspI PacI PasI PflMI
PfoI PmlI PsiI PspOMI PstI SapI ScaI SexAI
SfcI SfoI SmlI SnaBI SphI SspI StyI TaqII
TatI TstI Tth111I ZraI
Enzymes that do not cut:
Acc65I AcuI AfeI AgeI AleI AscI AsiSI AvaI
AvrII BamHI BbvCI BcgI BciVI BfrBI BglI BmrI
BmtI Bpu10I BsaI BsiWI BsrDI BsrGI BssHII BstBI
BstEII BstXI Bsu36I BtgZI ClaI CspCI DraIII EagI
EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HpaI
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PciI PmeI PpiI PpuMI
PshAI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SfiI SgfI SgrAI SmaI SpeI
SrfI StuI SwaI XbaI XcmI XhoI XmaI XmnI