Restriction map for
pF1KB7020
(Linear) (Six-base) MAPSORT of: KIBB7020.seq Check: 9589 from: 1 to: 2994
KIBB7020.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 964 2994
Size: 727 237 2030
Fragments arranged by size:
2030 727 237
AccI GT'mk_AC
Cuts at: 0 314 1950 2994
Size: 314 1636 1044
Fragments arranged by size:
1636 1044 314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 2020 2994
Size: 1382 638 974
Fragments arranged by size:
1382 974 638
AfeI AGC'GCT
Cuts at: 0 2721 2994
Size: 2721 273
AflIII A'CryG_T
Cuts at: 0 1886 2994
Size: 1886 1108
AleI CACnn'nnGTG
Cuts at: 0 529 1012 2994
Size: 529 483 1982
Fragments arranged by size:
1982 529 483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1030 1817 2994
Size: 1030 787 1177
Fragments arranged by size:
1177 1030 787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 2994
Size: 313 32 2649
Fragments arranged by size:
2649 313 32
AlwNI CAG_nnn'CTG
Cuts at: 0 950 1004 1585 2979 2994
Size: 950 54 581 1394 15
Fragments arranged by size:
1394 950 581 54 15
ApaI G_GGCC'C
Cuts at: 0 899 1650 2994
Size: 899 751 1344
Fragments arranged by size:
1344 899 751
ApaLI G'TGCA_C
Cuts at: 0 2263 2994
Size: 2263 731
ApoI r'AATT_y
Cuts at: 0 1927 2650 2994
Size: 1927 723 344
Fragments arranged by size:
1927 723 344
AvaI C'yCGr_G
Cuts at: 0 667 1028 1069 1085 1293 2089 2994
Size: 667 361 41 16 208 796 905
Fragments arranged by size:
905 796 667 361 208 41 16
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 909 942 2994
Size: 909 33 2052
Fragments arranged by size:
2052 909 33
BanI G'GyrC_C
Cuts at: 0 589 715 747 789 799 1419 2994
Size: 589 126 32 42 10 620 1575
Fragments arranged by size:
1575 620 589 126 42 32 10
BanII G_rGCy'C
Cuts at: 0 12 326 692 899 1085 1306 1650 1687
Size: 12 314 366 207 186 221 344 37
Cuts at: 1687 2994
Size: 1307
Fragments arranged by size:
1307 366 344 314 221 207 186 37
12
BbeI G_GCGC'C
Cuts at: 0 593 2994
Size: 593 2401
BbsI GAAGACnn'nnnn_
Cuts at: 0 1698 2894 2994
Size: 1698 1196 100
Fragments arranged by size:
1698 1196 100
BbvCI CC'TCA_GC
Cuts at: 0 426 1158 2994
Size: 426 732 1836
Fragments arranged by size:
1836 732 426
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2260 2294 2994
Size: 2260 34 700
Fragments arranged by size:
2260 700 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2956 2994
Size: 2956 38
BclI T'GATC_A
Cuts at: 0 2067 2994
Size: 2067 927
BglI GCCn_nnn'nGGC
Cuts at: 0 42 2422 2994
Size: 42 2380 572
Fragments arranged by size:
2380 572 42
Bme1580I G_kGCm'C
Cuts at: 0 899 1370 1422 1575 1650 2267 2994
Size: 899 471 52 153 75 617 727
Fragments arranged by size:
899 727 617 471 153 75 52
BmgBI CAC'GTC
Cuts at: 0 1887 2994
Size: 1887 1107
BmrI ACTGGGnnnn_n'
Cuts at: 0 689 840 2526 2994
Size: 689 151 1686 468
Fragments arranged by size:
1686 689 468 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 513 711 1656 2994
Size: 513 198 945 1338
Fragments arranged by size:
1338 945 513 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1642 2368 2484 2994
Size: 1169 473 726 116 510
Fragments arranged by size:
1169 726 510 473 116
Bpu10I CC'TnA_GC
Cuts at: 0 426 1158 1392 2038 2938 2994
Size: 426 732 234 646 900 56
Fragments arranged by size:
900 732 646 426 234 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 739 2689 2994
Size: 310 429 1950 305
Fragments arranged by size:
1950 429 310 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 376 664 1615 2994
Size: 112 264 288 951 1379
Fragments arranged by size:
1379 951 288 264 112
BsaAI yAC'GTr
Cuts at: 0 318 462 2994
Size: 318 144 2532
Fragments arranged by size:
2532 318 144
BsaBI GATnn'nnATC
Cuts at: 0 2401 2994
Size: 2401 593
BsaHI Gr'CG_yC
Cuts at: 0 590 1547 2994
Size: 590 957 1447
Fragments arranged by size:
1447 957 590
BsaWI w'CCGG_w
Cuts at: 0 2633 2994
Size: 2633 361
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 832 862 2729 2759 2994
Size: 313 30 489 30 1867 30 235
Fragments arranged by size:
1867 489 313 235 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 423 1921 2994
Size: 423 1498 1073
Fragments arranged by size:
1498 1073 423
BseYI C'CCAG_C
Cuts at: 0 936 1206 1649 1696 1921 2621 2994
Size: 936 270 443 47 225 700 373
Fragments arranged by size:
936 700 443 373 270 225 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 789 936 990 998 1294 1366 1543
Size: 325 464 147 54 8 296 72 177
Cuts at: 1543 2584 2994
Size: 1041 410
Fragments arranged by size:
1041 464 410 325 296 177 147 72
54 8
BsiEI CG_ry'CG
Cuts at: 0 516 1328 2994
Size: 516 812 1666
Fragments arranged by size:
1666 812 516
BsiHKAI G_wGCw'C
Cuts at: 0 326 2267 2994
Size: 326 1941 727
Fragments arranged by size:
1941 727 326
Bsp1286I G_dGCh'C
Cuts at: 0 12 326 692 899 1085 1306 1370 1422
Size: 12 314 366 207 186 221 64 52
Cuts at: 1422 1575 1650 1687 2267 2994
Size: 153 75 37 580 727
Fragments arranged by size:
727 580 366 314 221 207 186 153
75 64 52 37 12
BspHI T'CATG_A
Cuts at: 0 2492 2994
Size: 2492 502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 964 2900 2979 2994
Size: 727 237 1936 79 15
Fragments arranged by size:
1936 727 237 79 15
BsrBI CCG'CTC
Cuts at: 0 1431 2994
Size: 1431 1563
BsrDI GCAATG_nn'
Cuts at: 0 476 704 1799 2222 2697 2711 2994
Size: 476 228 1095 423 475 14 283
Fragments arranged by size:
1095 476 475 423 283 228 14
BsrFI r'CCGG_y
Cuts at: 0 97 511 817 2230 2564 2917 2994
Size: 97 414 306 1413 334 353 77
Fragments arranged by size:
1413 414 353 334 306 97 77
BsrGI T'GTAC_A
Cuts at: 0 1115 2994
Size: 1115 1879
BssHII G'CGCG_C
Cuts at: 0 615 2994
Size: 615 2379
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1136 2994
Size: 1136 1858
BstEII G'GTnAC_C
Cuts at: 0 2100 2907 2994
Size: 2100 807 87
Fragments arranged by size:
2100 807 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 1147 2385 2814 2994
Size: 699 448 1238 429 180
Fragments arranged by size:
1238 699 448 429 180
BstYI r'GATC_y
Cuts at: 0 2396 2933 2994
Size: 2396 537 61
Fragments arranged by size:
2396 537 61
Bsu36I CC'TnA_GG
Cuts at: 0 1744 2641 2994
Size: 1744 897 353
Fragments arranged by size:
1744 897 353
BtgI C'CryG_G
Cuts at: 0 734 1140 1944 2549 2579 2994
Size: 734 406 804 605 30 415
Fragments arranged by size:
804 734 605 415 406 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 431 788 1473 1713 2994
Size: 431 357 685 240 1281
Fragments arranged by size:
1281 685 431 357 240
BtsI GCAGTG_nn'
Cuts at: 0 2596 2994
Size: 2596 398
DraIII CAC_nnn'GTG
Cuts at: 0 1174 2994
Size: 1174 1820
DrdI GACnn_nn'nnGTC
Cuts at: 0 2758 2994
Size: 2758 236
EaeI y'GGCC_r
Cuts at: 0 2 513 819 1098 1325 1965 1985 2113
Size: 2 511 306 279 227 640 20 128
Cuts at: 2113 2915 2919 2994
Size: 802 4 75
Fragments arranged by size:
802 640 511 306 279 227 128 75
20 4 2
EagI C'GGCC_G
Cuts at: 0 513 1325 2994
Size: 513 812 1669
Fragments arranged by size:
1669 812 513
EarI CTCTTCn'nnn_
Cuts at: 0 110 182 400 667 1471 2994
Size: 110 72 218 267 804 1523
Fragments arranged by size:
1523 804 267 218 110 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 700 2994
Size: 700 2294
EcoICRI GAG'CTC
Cuts at: 0 324 2994
Size: 324 2670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1382 1642 2020 2368 2484 2994
Size: 1169 213 260 378 348 116 510
Fragments arranged by size:
1169 510 378 348 260 213 116
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2041 2994
Size: 2041 953
EcoO109I rG'GnC_Cy
Cuts at: 0 895 896 1089 1154 1646 1845 2994
Size: 895 1 193 65 492 199 1149
Fragments arranged by size:
1149 895 492 199 193 65 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 402 579 2850 2994
Size: 402 177 2271 144
Fragments arranged by size:
2271 402 177 144
FseI GG_CCGG'CC
Cuts at: 0 2921 2994
Size: 2921 73
FspI TGC'GCA
Cuts at: 0 535 2994
Size: 535 2459
HaeII r_GCGC'y
Cuts at: 0 112 593 1224 2723 2994
Size: 112 481 631 1499 271
Fragments arranged by size:
1499 631 481 271 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 409 441 973 1005 1022 1054
Size: 153 32 224 32 532 32 17 32
Cuts at: 1054 2344 2376 2994
Size: 1290 32 618
Fragments arranged by size:
1290 618 532 224 153 32 32 32
32 32 17
HincII GTy'rAC
Cuts at: 0 522 1284 1951 2298 2994
Size: 522 762 667 347 696
Fragments arranged by size:
762 696 667 522 347
KasI G'GCGC_C
Cuts at: 0 589 2994
Size: 589 2405
MscI TGG'CCA
Cuts at: 0 4 1987 2994
Size: 4 1983 1007
Fragments arranged by size:
1983 1007 4
MslI CAynn'nnrTG
Cuts at: 0 529 610 1012 2812 2994
Size: 529 81 402 1800 182
Fragments arranged by size:
1800 529 402 182 81
MspA1I CmG'CkG
Cuts at: 0 54 66 81 309 832 1206 1527 1696
Size: 54 12 15 228 523 374 321 169
Cuts at: 1696 2025 2202 2408 2994
Size: 329 177 206 586
Fragments arranged by size:
586 523 374 329 321 228 206 177
169 54 15 12
NaeI GCC'GGC
Cuts at: 0 99 513 2232 2919 2994
Size: 99 414 1719 687 75
Fragments arranged by size:
1719 687 414 99 75
NarI GG'CG_CC
Cuts at: 0 590 2994
Size: 590 2404
NcoI C'CATG_G
Cuts at: 0 1140 2579 2994
Size: 1140 1439 415
Fragments arranged by size:
1439 1140 415
NgoMIV G'CCGG_C
Cuts at: 0 97 511 2230 2917 2994
Size: 97 414 1719 687 77
Fragments arranged by size:
1719 687 414 97 77
NspI r_CATG'y
Cuts at: 0 600 2214 2736 2958 2994
Size: 600 1614 522 222 36
Fragments arranged by size:
1614 600 522 222 36
PasI CC'CwG_GG
Cuts at: 0 865 2385 2994
Size: 865 1520 609
Fragments arranged by size:
1520 865 609
PflMI CCAn_nnn'nTGG
Cuts at: 0 178 1245 2205 2408 2994
Size: 178 1067 960 203 586
Fragments arranged by size:
1067 960 586 203 178
PfoI T'CCnGG_A
Cuts at: 0 578 1448 1658 1806 2345 2747 2948 2994
Size: 578 870 210 148 539 402 201 46
Fragments arranged by size:
870 578 539 402 210 201 148 46
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 2994
Size: 313 32 2649
Fragments arranged by size:
2649 313 32
PpuMI rG'GwC_Cy
Cuts at: 0 1089 1845 2994
Size: 1089 756 1149
Fragments arranged by size:
1149 1089 756
PspOMI G'GGCC_C
Cuts at: 0 895 1646 2994
Size: 895 751 1348
Fragments arranged by size:
1348 895 751
PspXI vC'TCGA_Gb
Cuts at: 0 1069 2089 2994
Size: 1069 1020 905
Fragments arranged by size:
1069 1020 905
PstI C_TGCA'G
Cuts at: 0 1793 2966 2994
Size: 1793 1173 28
Fragments arranged by size:
1793 1173 28
PvuII CAG'CTG
Cuts at: 0 832 1206 1527 1696 2202 2994
Size: 832 374 321 169 506 792
Fragments arranged by size:
832 792 506 374 321 169
SacI G_AGCT'C
Cuts at: 0 326 2994
Size: 326 2668
SalI G'TCGA_C
Cuts at: 0 1949 2994
Size: 1949 1045
SapI GCTCTTCn'nnn_
Cuts at: 0 1471 2994
Size: 1471 1523
ScaI AGT'ACT
Cuts at: 0 1468 2994
Size: 1468 1526
SexAI A'CCwGG_T
Cuts at: 0 233 2306 2864 2994
Size: 233 2073 558 130
Fragments arranged by size:
2073 558 233 130
SfcI C'TryA_G
Cuts at: 0 1691 1789 2962 2994
Size: 1691 98 1173 32
Fragments arranged by size:
1691 1173 98 32
SfoI GGC'GCC
Cuts at: 0 591 2994
Size: 591 2403
SmaI CCC'GGG
Cuts at: 0 1087 2994
Size: 1087 1907
SmlI C'TyrA_G
Cuts at: 0 325 754 1069 2089 2704 2994
Size: 325 429 315 1020 615 290
Fragments arranged by size:
1020 615 429 325 315 290
StuI AGG'CCT
Cuts at: 0 2047 2994
Size: 2047 947
StyI C'CwwG_G
Cuts at: 0 527 554 836 1140 1874 2579 2994
Size: 527 27 282 304 734 705 415
Fragments arranged by size:
734 705 527 415 304 282 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 170 787 1084 2592 2613 2994
Size: 170 617 297 1508 21 381
Fragments arranged by size:
1508 617 381 297 170 21
TatI w'GTAC_w
Cuts at: 0 1115 1466 1598 1775 1796 2994
Size: 1115 351 132 177 21 1198
Fragments arranged by size:
1198 1115 351 177 132 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 832 864 2729 2761 2994
Size: 832 32 1865 32 233
Fragments arranged by size:
1865 832 233 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 179 2994
Size: 179 2815
XhoI C'TCGA_G
Cuts at: 0 1069 2089 2994
Size: 1069 1020 905
Fragments arranged by size:
1069 1020 905
XmaI C'CCGG_G
Cuts at: 0 1085 2994
Size: 1085 1909
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SalI SapI ScaI SexAI
SfcI SfoI SmaI SmlI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AscI AseI
AsiSI AvrII BamHI BfrBI BglII BlpI BmtI BsiWI
BsmI BsmBI BspEI BssSI BstBI BstZ17I ClaI CspCI
DraI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PshAI PsiI PsrI PvuI RsrII
SacII SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XmnI ZraI