Restriction map for
pF1KB7022
(Linear) (Six-base) MAPSORT of: KIBB7022.seq Check: 6352 from: 1 to: 2916
KIBB7022.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 0 2513 2916
Size: 2513 403
AccI GT'mk_AC
Cuts at: 0 2090 2492 2916
Size: 2090 402 424
Fragments arranged by size:
2090 424 402
Acc65I G'GTAC_C
Cuts at: 0 1268 2916
Size: 1268 1648
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1852 1873 1921 2713 2916
Size: 1132 720 21 48 792 203
Fragments arranged by size:
1132 792 720 203 48 21
AhdI GACnn_n'nnGTC
Cuts at: 0 2363 2514 2916
Size: 2363 151 402
Fragments arranged by size:
2363 402 151
AleI CACnn'nnGTG
Cuts at: 0 1549 2167 2916
Size: 1549 618 749
Fragments arranged by size:
1549 749 618
AlwNI CAG_nnn'CTG
Cuts at: 0 1410 2916
Size: 1410 1506
ApaI G_GGCC'C
Cuts at: 0 7 605 1461 2916
Size: 7 598 856 1455
Fragments arranged by size:
1455 856 598 7
AvaI C'yCGr_G
Cuts at: 0 2077 2110 2916
Size: 2077 33 806
Fragments arranged by size:
2077 806 33
BanI G'GyrC_C
Cuts at: 0 621 1243 1268 2916
Size: 621 622 25 1648
Fragments arranged by size:
1648 622 621 25
BanII G_rGCy'C
Cuts at: 0 7 74 605 913 956 1046 1173 1187
Size: 7 67 531 308 43 90 127 14
Cuts at: 1187 1385 1461 1515 2065 2255 2690 2839 2916
Size: 198 76 54 550 190 435 149 77
Fragments arranged by size:
550 531 435 308 198 190 149 127
90 77 76 67 54 43 14 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 2916
Size: 696 2220
BbvCI CC'TCA_GC
Cuts at: 0 205 2036 2916
Size: 205 1831 880
Fragments arranged by size:
1831 880 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 395 429 2916
Size: 395 34 2487
Fragments arranged by size:
2487 395 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2608 2916
Size: 2608 308
BglI GCCn_nnn'nGGC
Cuts at: 0 1919 2677 2916
Size: 1919 758 239
Fragments arranged by size:
1919 758 239
BglII A'GATC_T
Cuts at: 0 2540 2714 2916
Size: 2540 174 202
Fragments arranged by size:
2540 202 174
BlpI GC'TnA_GC
Cuts at: 0 672 2916
Size: 672 2244
Bme1580I G_kGCm'C
Cuts at: 0 7 537 605 1461 2916
Size: 7 530 68 856 1455
Fragments arranged by size:
1455 856 530 68 7
BmgBI CAC'GTC
Cuts at: 0 2334 2916
Size: 2334 582
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 68 2916
Size: 55 13 2848
Fragments arranged by size:
2848 55 13
BmtI G_CTAG'C
Cuts at: 0 334 2916
Size: 334 2582
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2562 2853 2916
Size: 2562 291 63
Fragments arranged by size:
2562 291 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 281 1185 1790 2130 2259 2269 2845
Size: 177 104 904 605 340 129 10 576
Cuts at: 2845 2916
Size: 71
Fragments arranged by size:
904 605 576 340 177 129 104 71
10
Bpu10I CC'TnA_GC
Cuts at: 0 83 205 1040 1372 2036 2059 2916
Size: 83 122 835 332 664 23 857
Fragments arranged by size:
857 835 664 332 122 83 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 2916
Size: 927 1989
BsaI GGTCTCn'nnnn_
Cuts at: 0 257 1417 2916
Size: 257 1160 1499
Fragments arranged by size:
1499 1160 257
BsaBI GATnn'nnATC
Cuts at: 0 562 2916
Size: 562 2354
BsaHI Gr'CG_yC
Cuts at: 0 410 2087 2510 2916
Size: 410 1677 423 406
Fragments arranged by size:
1677 423 410 406
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 95 125 850 880 1958 1988 2916
Size: 95 30 725 30 1078 30 928
Fragments arranged by size:
1078 928 725 95 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2521 2842 2845 2916
Size: 2521 321 3 71
Fragments arranged by size:
2521 321 71 3
BseYI C'CCAG_C
Cuts at: 0 606 1274 2046 2053 2627 2675 2879 2916
Size: 606 668 772 7 574 48 204 37
Fragments arranged by size:
772 668 606 574 204 48 37 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 234 604 868 1320 1423 1473 2916
Size: 234 370 264 452 103 50 1443
Fragments arranged by size:
1443 452 370 264 234 103 50
BsiHKAI G_wGCw'C
Cuts at: 0 673 913 1173 1187 1436 2255 2839 2849
Size: 673 240 260 14 249 819 584 10
Cuts at: 2849 2916
Size: 67
Fragments arranged by size:
819 673 584 260 249 240 67 14
10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 417 2916
Size: 417 2499
Bsp1286I G_dGCh'C
Cuts at: 0 7 74 537 605 673 913 956 1046
Size: 7 67 463 68 68 240 43 90
Cuts at: 1046 1173 1187 1385 1436 1461 1515 2065 2255
Size: 127 14 198 51 25 54 550 190
Cuts at: 2255 2690 2839 2849 2916
Size: 435 149 10 67
Fragments arranged by size:
550 463 435 240 198 190 149 127
90 68 68 67 67 54 51 43
25 14 10 7
BspHI T'CATG_A
Cuts at: 0 1907 2408 2916
Size: 1907 501 508
Fragments arranged by size:
1907 508 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1332 1751 2018 2270 2863 2916
Size: 1332 419 267 252 593 53
Fragments arranged by size:
1332 593 419 267 252 53
BsrBI CCG'CTC
Cuts at: 0 2760 2916
Size: 2760 156
BsrDI GCAATG_nn'
Cuts at: 0 138 2445 2916
Size: 138 2307 471
Fragments arranged by size:
2307 471 138
BsrFI r'CCGG_y
Cuts at: 0 434 817 2916
Size: 434 383 2099
Fragments arranged by size:
2099 434 383
BsrGI T'GTAC_A
Cuts at: 0 1610 2916
Size: 1610 1306
BssSI C'ACGA_G
Cuts at: 0 1927 2916
Size: 1927 989
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1410 2916
Size: 1410 1506
BstEII G'GTnAC_C
Cuts at: 0 111 936 2916
Size: 111 825 1980
Fragments arranged by size:
1980 825 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1163 2916
Size: 1163 1753
BstYI r'GATC_y
Cuts at: 0 2540 2714 2916
Size: 2540 174 202
Fragments arranged by size:
2540 202 174
Bsu36I CC'TnA_GG
Cuts at: 0 1364 2916
Size: 1364 1552
BtgI C'CryG_G
Cuts at: 0 941 1796 1859 1962 2916
Size: 941 855 63 103 954
Fragments arranged by size:
954 941 855 103 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 135 2916
Size: 135 2781
BtsI GCAGTG_nn'
Cuts at: 0 537 1303 1308 2823 2916
Size: 537 766 5 1515 93
Fragments arranged by size:
1515 766 537 93 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1785 1820 2916
Size: 1785 35 1096
Fragments arranged by size:
1785 1096 35
DraIII CAC_nnn'GTG
Cuts at: 0 1395 2916
Size: 1395 1521
EaeI y'GGCC_r
Cuts at: 0 32 432 836 1128 1145 1305 2067 2372
Size: 32 400 404 292 17 160 762 305
Cuts at: 2372 2916
Size: 544
Fragments arranged by size:
762 544 404 400 305 292 160 32
17
EarI CTCTTCn'nnn_
Cuts at: 0 1546 2916
Size: 1546 1370
EcoICRI GAG'CTC
Cuts at: 0 911 1171 1185 2253 2837 2916
Size: 911 260 14 1068 584 79
Fragments arranged by size:
1068 911 584 260 79 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 281 1132 1185 1790 1852 1873 1921
Size: 177 104 851 53 605 62 21 48
Cuts at: 1921 2130 2259 2269 2713 2845 2916
Size: 209 129 10 444 132 71
Fragments arranged by size:
851 605 444 209 177 132 129 104
71 62 53 48 21 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 928 1375 2227 2916
Size: 928 447 852 689
Fragments arranged by size:
928 852 689 447
EcoO109I rG'GnC_Cy
Cuts at: 0 239 601 602 1018 1368 1457 1884 1969
Size: 239 362 1 416 350 89 427 85
Cuts at: 1969 2050 2073 2259 2916
Size: 81 23 186 657
Fragments arranged by size:
657 427 416 362 350 239 186 89
85 81 23 1
EcoRV GAT'ATC
Cuts at: 0 2874 2916
Size: 2874 42
FseI GG_CCGG'CC
Cuts at: 0 438 2916
Size: 438 2478
HaeII r_GCGC'y
Cuts at: 0 284 2916
Size: 284 2632
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2194 2226 2349 2381 2500 2532 2916
Size: 2194 32 123 32 119 32 384
Fragments arranged by size:
2194 384 123 119 32 32 32
HincII GTy'rAC
Cuts at: 0 693 1941 2091 2357 2916
Size: 693 1248 150 266 559
Fragments arranged by size:
1248 693 559 266 150
HindIII A'AGCT_T
Cuts at: 0 1048 2916
Size: 1048 1868
HpaI GTT'AAC
Cuts at: 0 693 2916
Size: 693 2223
KpnI G_GTAC'C
Cuts at: 0 1272 2916
Size: 1272 1644
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 834 2444 2916
Size: 735 99 1610 472
Fragments arranged by size:
1610 735 472 99
MscI TGG'CCA
Cuts at: 0 34 838 1147 1307 2374 2916
Size: 34 804 309 160 1067 542
Fragments arranged by size:
1067 804 542 309 160 34
MslI CAynn'nnrTG
Cuts at: 0 296 868 1549 1871 2167 2578 2916
Size: 296 572 681 322 296 411 338
Fragments arranged by size:
681 572 411 338 322 296 296
MspA1I CmG'CkG
Cuts at: 0 1646 1704 2804 2916
Size: 1646 58 1100 112
Fragments arranged by size:
1646 1100 112 58
NaeI GCC'GGC
Cuts at: 0 436 819 2916
Size: 436 383 2097
Fragments arranged by size:
2097 436 383
NcoI C'CATG_G
Cuts at: 0 1962 2916
Size: 1962 954
NdeI CA'TA_TG
Cuts at: 0 1219 2916
Size: 1219 1697
NgoMIV G'CCGG_C
Cuts at: 0 434 817 2916
Size: 434 383 2099
Fragments arranged by size:
2099 434 383
NheI G'CTAG_C
Cuts at: 0 330 2916
Size: 330 2586
NspI r_CATG'y
Cuts at: 0 817 1569 2916
Size: 817 752 1347
Fragments arranged by size:
1347 817 752
PasI CC'CwG_GG
Cuts at: 0 270 598 2289 2916
Size: 270 328 1691 627
Fragments arranged by size:
1691 627 328 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 41 2157 2295 2916
Size: 41 2116 138 621
Fragments arranged by size:
2116 621 138 41
PfoI T'CCnGG_A
Cuts at: 0 929 2255 2916
Size: 929 1326 661
Fragments arranged by size:
1326 929 661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 895 927 2916
Size: 895 32 1989
Fragments arranged by size:
1989 895 32
PpuMI rG'GwC_Cy
Cuts at: 0 239 1018 1368 2050 2073 2259 2916
Size: 239 779 350 682 23 186 657
Fragments arranged by size:
779 682 657 350 239 186 23
PspOMI G'GGCC_C
Cuts at: 0 3 601 1457 2916
Size: 3 598 856 1459
Fragments arranged by size:
1459 856 598 3
PstI C_TGCA'G
Cuts at: 0 630 1298 1343 1509 1748 2894 2916
Size: 630 668 45 166 239 1146 22
Fragments arranged by size:
1146 668 630 239 166 45 22
PvuII CAG'CTG
Cuts at: 0 1704 2916
Size: 1704 1212
SacI G_AGCT'C
Cuts at: 0 913 1173 1187 2255 2839 2916
Size: 913 260 14 1068 584 77
Fragments arranged by size:
1068 913 584 260 77 14
SalI G'TCGA_C
Cuts at: 0 2089 2916
Size: 2089 827
SanDI GG'GwC_CC
Cuts at: 0 1018 2050 2916
Size: 1018 1032 866
Fragments arranged by size:
1032 1018 866
ScaI AGT'ACT
Cuts at: 0 1993 2916
Size: 1993 923
SexAI A'CCwGG_T
Cuts at: 0 1637 2916
Size: 1637 1279
SfcI C'TryA_G
Cuts at: 0 626 1294 1339 1505 1744 2890 2916
Size: 626 668 45 166 239 1146 26
Fragments arranged by size:
1146 668 626 239 166 45 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2677 2916
Size: 2677 239
SmlI C'TyrA_G
Cuts at: 0 906 2110 2916
Size: 906 1204 806
Fragments arranged by size:
1204 906 806
StuI AGG'CCT
Cuts at: 0 979 989 2181 2671 2916
Size: 979 10 1192 490 245
Fragments arranged by size:
1192 979 490 245 10
StyI C'CwwG_G
Cuts at: 0 1067 1962 2681 2916
Size: 1067 895 719 235
Fragments arranged by size:
1067 895 719 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 2707 2916
Size: 532 2175 209
Fragments arranged by size:
2175 532 209
TatI w'GTAC_w
Cuts at: 0 179 1610 1974 1991 2916
Size: 179 1431 364 17 925
Fragments arranged by size:
1431 925 364 179 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 47 79 95 127 850 882 2916
Size: 47 32 16 32 723 32 2034
Fragments arranged by size:
2034 723 47 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 0 2488 2916
Size: 2488 428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 167 2154 2367 2916
Size: 167 1987 213 549
Fragments arranged by size:
1987 549 213 167
XhoI C'TCGA_G
Cuts at: 0 2110 2916
Size: 2110 806
ZraI GAC'GTC
Cuts at: 0 2511 2916
Size: 2511 405
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AleI AlwNI ApaI
AvaI BanI BanII BbsI BbvCI BcgI BciVI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FseI HaeII Hin4I HincII
HindIII HpaI KpnI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NspI PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SalI SanDI
ScaI SexAI SfcI SfiI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BclI BfrBI BsaAI BsaWI BsiEI BsiWI BsmI
BspEI BssHII BstBI BstZ17I ClaI DraI DrdI EagI
EciI EcoRI FalI FspI FspAI KasI MfeI MluI
NarI NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SapI
SbfI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XmaI XmnI