Restriction map for
pF1KB7023
(Linear) (Six-base) MAPSORT of: KIBB7023.seq Check: 3214 from: 1 to: 3318
KIBB7023.orf
With 182 enzymes: SgfI *
July 23, 2007 10:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 877 1827 2904 3318
Size: 877 950 1077 414
Fragments arranged by size:
1077 950 877 414
AatII G_ACGT'C
Cuts at: 0 2282 3318
Size: 2282 1036
AccI GT'mk_AC
Cuts at: 0 617 3318
Size: 617 2701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1975 2400 3318
Size: 1975 425 918
Fragments arranged by size:
1975 918 425
AfeI AGC'GCT
Cuts at: 0 1083 3318
Size: 1083 2235
AflIII A'CryG_T
Cuts at: 0 1112 2654 3318
Size: 1112 1542 664
Fragments arranged by size:
1542 1112 664
AhdI GACnn_n'nnGTC
Cuts at: 0 2298 2658 3318
Size: 2298 360 660
Fragments arranged by size:
2298 660 360
AleI CACnn'nnGTG
Cuts at: 0 527 3318
Size: 527 2791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2173 2608 3318
Size: 2173 435 710
Fragments arranged by size:
2173 710 435
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1283 1315 3318
Size: 1283 32 2003
Fragments arranged by size:
2003 1283 32
AlwNI CAG_nnn'CTG
Cuts at: 0 96 1171 3318
Size: 96 1075 2147
Fragments arranged by size:
2147 1075 96
ApaI G_GGCC'C
Cuts at: 0 1979 3109 3318
Size: 1979 1130 209
Fragments arranged by size:
1979 1130 209
AvaI C'yCGr_G
Cuts at: 0 111 162 855 2543 2872 3088 3290 3318
Size: 111 51 693 1688 329 216 202 28
Fragments arranged by size:
1688 693 329 216 202 111 51 28
BamHI G'GATC_C
Cuts at: 0 2669 3318
Size: 2669 649
BanI G'GyrC_C
Cuts at: 0 235 1569 2701 3278 3318
Size: 235 1334 1132 577 40
Fragments arranged by size:
1334 1132 577 235 40
BanII G_rGCy'C
Cuts at: 0 206 1720 1979 2144 2171 3109 3197 3221
Size: 206 1514 259 165 27 938 88 24
Cuts at: 3221 3233 3318
Size: 12 85
Fragments arranged by size:
1514 938 259 206 165 88 85 27
24 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 3318
Size: 857 2461
BbvCI CC'TCA_GC
Cuts at: 0 1208 3318
Size: 1208 2110
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 727 1483 1517 3318
Size: 693 34 756 34 1801
Fragments arranged by size:
1801 756 693 34 34
BclI T'GATC_A
Cuts at: 0 519 2372 3318
Size: 519 1853 946
Fragments arranged by size:
1853 946 519
BglI GCCn_nnn'nGGC
Cuts at: 0 1615 2166 3318
Size: 1615 551 1152
Fragments arranged by size:
1615 1152 551
BglII A'GATC_T
Cuts at: 0 83 2522 2684 2795 3318
Size: 83 2439 162 111 523
Fragments arranged by size:
2439 523 162 111 83
BlpI GC'TnA_GC
Cuts at: 0 983 2392 3318
Size: 983 1409 926
Fragments arranged by size:
1409 983 926
Bme1580I G_kGCm'C
Cuts at: 0 970 1979 2258 3109 3283 3318
Size: 970 1009 279 851 174 35
Fragments arranged by size:
1009 970 851 279 174 35
BmgBI CAC'GTC
Cuts at: 0 2655 3318
Size: 2655 663
BmrI ACTGGGnnnn_n'
Cuts at: 0 281 830 1455 3318
Size: 281 549 625 1863
Fragments arranged by size:
1863 625 549 281
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1889 3318
Size: 1889 1429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 228 343 610 1062 1279 1447 2369
Size: 123 105 115 267 452 217 168 922
Cuts at: 2369 3133 3211 3217 3318
Size: 764 78 6 101
Fragments arranged by size:
922 764 452 267 217 168 123 115
105 101 78 6
Bpu10I CC'TnA_GC
Cuts at: 0 1208 1864 2647 3318
Size: 1208 656 783 671
Fragments arranged by size:
1208 783 671 656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3247 3318
Size: 3247 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 319 1114 1349 2054 2468 3318
Size: 319 795 235 705 414 850
Fragments arranged by size:
850 795 705 414 319 235
BsaAI yAC'GTr
Cuts at: 0 945 1375 1497 1743 1775 3318
Size: 945 430 122 246 32 1543
Fragments arranged by size:
1543 945 430 246 122 32
BsaHI Gr'CG_yC
Cuts at: 0 2279 3318
Size: 2279 1039
BsaWI w'CCGG_w
Cuts at: 0 174 2613 3318
Size: 174 2439 705
Fragments arranged by size:
2439 705 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 295 325 802 832 1111 1141 1447 1477
Size: 295 30 477 30 279 30 306 30
Cuts at: 1477 2051 2081 2998 3028 3318
Size: 574 30 917 30 290
Fragments arranged by size:
917 574 477 306 295 290 279 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1429 1465 1468 2938 2949 3153 3318
Size: 1429 36 3 1470 11 204 165
Fragments arranged by size:
1470 1429 204 165 36 11 3
BseYI C'CCAG_C
Cuts at: 0 1325 1395 1803 2131 2179 2771 2819 2855
Size: 1325 70 408 328 48 592 48 36
Cuts at: 2855 2946 3124 3221 3318
Size: 91 178 97 97
Fragments arranged by size:
1325 592 408 328 178 97 97 91
70 48 48 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 682 811 1506 1995 2062 3055 3318
Size: 463 219 129 695 489 67 993 263
Fragments arranged by size:
993 695 489 463 263 219 129 67
BsiHKAI G_wGCw'C
Cuts at: 0 254 1480 1631 1720 2144 2498 3233 3318
Size: 254 1226 151 89 424 354 735 85
Fragments arranged by size:
1226 735 424 354 254 151 89 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 651 2270 3318
Size: 651 1619 1048
Fragments arranged by size:
1619 1048 651
Bsp1286I G_dGCh'C
Cuts at: 0 206 254 970 1480 1631 1720 1979 2144
Size: 206 48 716 510 151 89 259 165
Cuts at: 2144 2171 2258 2498 3109 3197 3221 3233 3283
Size: 27 87 240 611 88 24 12 50
Cuts at: 3283 3318
Size: 35
Fragments arranged by size:
716 611 510 259 240 206 165 151
89 88 87 50 48 35 27 24
12
BspEI T'CCGG_A
Cuts at: 0 2613 3318
Size: 2613 705
BspHI T'CATG_A
Cuts at: 0 1961 3318
Size: 1961 1357
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 166 877 999 1827 2084 2365 2904 3318
Size: 166 711 122 828 257 281 539 414
Fragments arranged by size:
828 711 539 414 281 257 166 122
BsrBI CCG'CTC
Cuts at: 0 336 818 938 3318
Size: 336 482 120 2380
Fragments arranged by size:
2380 482 336 120
BsrDI GCAATG_nn'
Cuts at: 0 879 2157 3318
Size: 879 1278 1161
Fragments arranged by size:
1278 1161 879
BsrFI r'CCGG_y
Cuts at: 0 777 3318
Size: 777 2541
BssSI C'ACGA_G
Cuts at: 0 481 2407 3318
Size: 481 1926 911
Fragments arranged by size:
1926 911 481
BstEII G'GTnAC_C
Cuts at: 0 2758 3318
Size: 2758 560
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1395 2313 2439 3318
Size: 1395 918 126 879
Fragments arranged by size:
1395 918 879 126
BstYI r'GATC_y
Cuts at: 0 83 363 1688 2522 2669 2684 2795 3318
Size: 83 280 1325 834 147 15 111 523
Fragments arranged by size:
1325 834 523 280 147 111 83 15
BtgI C'CryG_G
Cuts at: 0 315 371 926 1786 1850 2985 3318
Size: 315 56 555 860 64 1135 333
Fragments arranged by size:
1135 860 555 333 315 64 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 31 3318
Size: 31 3287
BtsI GCAGTG_nn'
Cuts at: 0 475 502 665 1003 1480 1932 3318
Size: 475 27 163 338 477 452 1386
Fragments arranged by size:
1386 477 475 452 338 163 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 741 776 1562 1597 3318
Size: 741 35 786 35 1721
Fragments arranged by size:
1721 786 741 35 35
EaeI y'GGCC_r
Cuts at: 0 218 1173 1311 1504 1725 1892 2429 3318
Size: 218 955 138 193 221 167 537 889
Fragments arranged by size:
955 889 537 221 218 193 167 138
EarI CTCTTCn'nnn_
Cuts at: 0 1406 2819 3318
Size: 1406 1413 499
Fragments arranged by size:
1413 1406 499
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 664 3310 3318
Size: 664 2646 8
Fragments arranged by size:
2646 664 8
EcoICRI GAG'CTC
Cuts at: 0 1718 2142 3231 3318
Size: 1718 424 1089 87
Fragments arranged by size:
1718 1089 424 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 228 343 610 1062 1279 1447 1975
Size: 123 105 115 267 452 217 168 528
Cuts at: 1975 2369 2400 3133 3211 3217 3318
Size: 394 31 733 78 6 101
Fragments arranged by size:
733 528 452 394 267 217 168 123
115 105 101 78 31 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 958 3318
Size: 958 2360
EcoO109I rG'GnC_Cy
Cuts at: 0 93 860 1826 1976 2006 2023 2354 3105
Size: 93 767 966 150 30 17 331 751
Cuts at: 3105 3318
Size: 213
Fragments arranged by size:
966 767 751 331 213 150 93 30
17
FspI TGC'GCA
Cuts at: 0 1531 3318
Size: 1531 1787
HaeII r_GCGC'y
Cuts at: 0 1085 2124 3318
Size: 1085 1039 1194
Fragments arranged by size:
1194 1085 1039
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 9 41 1799 1831 1950 1982 2012 2044
Size: 9 32 1758 32 119 32 30 32
Cuts at: 2044 2284 2316 2644 2676 2998 3030 3318
Size: 240 32 328 32 322 32 288
Fragments arranged by size:
1758 328 322 288 240 119 32 32
32 32 32 32 32 30 9
HincII GTy'rAC
Cuts at: 0 729 1995 3156 3318
Size: 729 1266 1161 162
Fragments arranged by size:
1266 1161 729 162
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1928 2138 2330 2813 3318
Size: 1097 831 210 192 483 505
Fragments arranged by size:
1097 831 505 483 210 192
MscI TGG'CCA
Cuts at: 0 220 1175 2431 3318
Size: 220 955 1256 887
Fragments arranged by size:
1256 955 887 220
MslI CAynn'nnrTG
Cuts at: 0 355 527 697 902 925 1202 1567 1741
Size: 355 172 170 205 23 277 365 174
Cuts at: 1741 2224 3318
Size: 483 1094
Fragments arranged by size:
1094 483 365 355 277 205 174 172
170 23
MspA1I CmG'CkG
Cuts at: 0 465 1304 1425 1758 1788 2859 3318
Size: 465 839 121 333 30 1071 459
Fragments arranged by size:
1071 839 465 459 333 121 30
NcoI C'CATG_G
Cuts at: 0 2985 3318
Size: 2985 333
NspI r_CATG'y
Cuts at: 0 1323 3318
Size: 1323 1995
PasI CC'CwG_GG
Cuts at: 0 1068 3318
Size: 1068 2250
PflMI CCAn_nnn'nTGG
Cuts at: 0 2916 3318
Size: 2916 402
PmlI CAC'GTG
Cuts at: 0 1375 1497 1775 3318
Size: 1375 122 278 1543
Fragments arranged by size:
1543 1375 278 122
PpuMI rG'GwC_Cy
Cuts at: 0 860 2023 2354 3318
Size: 860 1163 331 964
Fragments arranged by size:
1163 964 860 331
PshAI GACnn'nnGTC
Cuts at: 0 670 3318
Size: 670 2648
PspOMI G'GGCC_C
Cuts at: 0 1975 3105 3318
Size: 1975 1130 213
Fragments arranged by size:
1975 1130 213
PstI C_TGCA'G
Cuts at: 0 1010 1354 1493 2105 3318
Size: 1010 344 139 612 1213
Fragments arranged by size:
1213 1010 612 344 139
PvuII CAG'CTG
Cuts at: 0 465 1425 1758 2859 3318
Size: 465 960 333 1101 459
Fragments arranged by size:
1101 960 465 459 333
SacI G_AGCT'C
Cuts at: 0 1720 2144 3233 3318
Size: 1720 424 1089 85
Fragments arranged by size:
1720 1089 424 85
SacII CC_GC'GG
Cuts at: 0 1789 3318
Size: 1789 1529
ScaI AGT'ACT
Cuts at: 0 2047 3318
Size: 2047 1271
SexAI A'CCwGG_T
Cuts at: 0 2063 2423 3318
Size: 2063 360 895
Fragments arranged by size:
2063 895 360
SfcI C'TryA_G
Cuts at: 0 618 1006 1231 1350 1489 2101 2145 3318
Size: 618 388 225 119 139 612 44 1173
Fragments arranged by size:
1173 618 612 388 225 139 119 44
SmaI CCC'GGG
Cuts at: 0 113 3318
Size: 113 3205
SmlI C'TyrA_G
Cuts at: 0 2543 2872 3226 3318
Size: 2543 329 354 92
Fragments arranged by size:
2543 354 329 92
SphI G_CATG'C
Cuts at: 0 1323 3318
Size: 1323 1995
StuI AGG'CCT
Cuts at: 0 1024 3318
Size: 1024 2294
StyI C'CwwG_G
Cuts at: 0 221 1971 2693 2985 3318
Size: 221 1750 722 292 333
Fragments arranged by size:
1750 722 333 292 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 343 2686 2948 2960 3318
Size: 316 27 2343 262 12 358
Fragments arranged by size:
2343 358 316 262 27 12
TatI w'GTAC_w
Cuts at: 0 1733 2045 3318
Size: 1733 312 1273
Fragments arranged by size:
1733 1273 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 295 327 494 526 802 834 2051 2083
Size: 295 32 167 32 276 32 1217 32
Cuts at: 2083 3262 3294 3318
Size: 1179 32 24
Fragments arranged by size:
1217 1179 295 276 167 32 32 32
32 32 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1282 1617 1887 2431 3318
Size: 1282 335 270 544 887
Fragments arranged by size:
1282 887 544 335 270
XhoI C'TCGA_G
Cuts at: 0 2543 2872 3318
Size: 2543 329 446
Fragments arranged by size:
2543 446 329
XmaI C'CCGG_G
Cuts at: 0 111 3318
Size: 111 3207
ZraI GAC'GTC
Cuts at: 0 2280 3318
Size: 2280 1038
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII MmeI MscI MslI MspA1I NcoI NspI
PasI PflMI PmlI PpuMI PshAI PspOMI PstI PvuII
SacI SacII ScaI SexAI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BfrBI BmtI BsaBI
BsiEI BsiWI BsmI BsrGI BssHII BstAPI BstBI BstZ17I
Bsu36I ClaI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XmnI