Restriction map for
pF1KB7031
(Linear) (Six-base) MAPSORT of: KIBB7031.seq Check: 8142 from: 1 to: 1119
KIBB7031.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 137 1119
Size: 137 982
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 864 1119
Size: 561 303 255
Fragments arranged by size:
561 303 255
AflIII A'CryG_T
Cuts at: 0 365 1119
Size: 365 754
AleI CACnn'nnGTG
Cuts at: 0 727 1119
Size: 727 392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 436 1119
Size: 436 683
AvaI C'yCGr_G
Cuts at: 0 157 1119
Size: 157 962
BanI G'GyrC_C
Cuts at: 0 818 1119
Size: 818 301
BanII G_rGCy'C
Cuts at: 0 851 1119
Size: 851 268
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 261 372 519 590 870 1119
Size: 217 44 111 147 71 280 249
Fragments arranged by size:
280 249 217 147 111 71 44
BclI T'GATC_A
Cuts at: 0 182 1119
Size: 182 937
Bme1580I G_kGCm'C
Cuts at: 0 823 1119
Size: 823 296
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 688 1119
Size: 321 367 431
Fragments arranged by size:
431 367 321
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 1119
Size: 528 591
BsaI GGTCTCn'nnnn_
Cuts at: 0 1099 1119
Size: 1099 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 824 854 1119
Size: 824 30 265
Fragments arranged by size:
824 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 1119
Size: 415 704
BseYI C'CCAG_C
Cuts at: 0 1042 1119
Size: 1042 77
BsiHKAI G_wGCw'C
Cuts at: 0 425 851 1119
Size: 425 426 268
Fragments arranged by size:
426 425 268
Bsp1286I G_dGCh'C
Cuts at: 0 425 823 851 1119
Size: 425 398 28 268
Fragments arranged by size:
425 398 268 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 554 1119
Size: 554 565
BsrDI GCAATG_nn'
Cuts at: 0 177 188 914 1119
Size: 177 11 726 205
Fragments arranged by size:
726 205 177 11
BsrFI r'CCGG_y
Cuts at: 0 644 1108 1119
Size: 644 464 11
Fragments arranged by size:
644 464 11
BssSI C'ACGA_G
Cuts at: 0 603 1119
Size: 603 516
BstAPI GCAn_nnn'nTGC
Cuts at: 0 149 1119
Size: 149 970
BstEII G'GTnAC_C
Cuts at: 0 111 1119
Size: 111 1008
BstYI r'GATC_y
Cuts at: 0 119 1119
Size: 119 1000
BtgI C'CryG_G
Cuts at: 0 299 1119
Size: 299 820
BtsI GCAGTG_nn'
Cuts at: 0 251 857 902 1119
Size: 251 606 45 217
Fragments arranged by size:
606 251 217 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 891 926 1119
Size: 891 35 193
Fragments arranged by size:
891 193 35
DraIII CAC_nnn'GTG
Cuts at: 0 654 1119
Size: 654 465
EaeI y'GGCC_r
Cuts at: 0 755 899 1119
Size: 755 144 220
Fragments arranged by size:
755 220 144
EarI CTCTTCn'nnn_
Cuts at: 0 994 1038 1119
Size: 994 44 81
Fragments arranged by size:
994 81 44
EcoICRI GAG'CTC
Cuts at: 0 849 1119
Size: 849 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 864 1119
Size: 561 303 255
Fragments arranged by size:
561 303 255
EcoO109I rG'GnC_Cy
Cuts at: 0 492 1119
Size: 492 627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 789 1119
Size: 789 330
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1119
Size: 76 32 1011
Fragments arranged by size:
1011 76 32
HindIII A'AGCT_T
Cuts at: 0 1053 1119
Size: 1053 66
MscI TGG'CCA
Cuts at: 0 757 901 1119
Size: 757 144 218
Fragments arranged by size:
757 218 144
MslI CAynn'nnrTG
Cuts at: 0 418 655 727 862 1119
Size: 418 237 72 135 257
Fragments arranged by size:
418 257 237 135 72
MspA1I CmG'CkG
Cuts at: 0 410 926 1119
Size: 410 516 193
Fragments arranged by size:
516 410 193
NaeI GCC'GGC
Cuts at: 0 646 1119
Size: 646 473
NcoI C'CATG_G
Cuts at: 0 299 1119
Size: 299 820
NgoMIV G'CCGG_C
Cuts at: 0 644 1119
Size: 644 475
NspI r_CATG'y
Cuts at: 0 369 1116 1119
Size: 369 747 3
Fragments arranged by size:
747 369 3
PciI A'CATG_T
Cuts at: 0 365 1119
Size: 365 754
PfoI T'CCnGG_A
Cuts at: 0 530 1119
Size: 530 589
PpuMI rG'GwC_Cy
Cuts at: 0 492 1119
Size: 492 627
PstI C_TGCA'G
Cuts at: 0 743 1119
Size: 743 376
PvuII CAG'CTG
Cuts at: 0 926 1119
Size: 926 193
SacI G_AGCT'C
Cuts at: 0 851 1119
Size: 851 268
SfcI C'TryA_G
Cuts at: 0 138 739 1119
Size: 138 601 380
Fragments arranged by size:
601 380 138
SmlI C'TyrA_G
Cuts at: 0 507 1119
Size: 507 612
SphI G_CATG'C
Cuts at: 0 1116 1119
Size: 1116 3
StyI C'CwwG_G
Cuts at: 0 98 299 1119
Size: 98 201 820
Fragments arranged by size:
820 201 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 1119
Size: 824 32 263
Fragments arranged by size:
824 263 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 78 123 1119
Size: 78 45 996
Fragments arranged by size:
996 78 45
XmnI GAAnn'nnTTC
Cuts at: 0 772 1119
Size: 772 347
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AvaI BanI BanII
BbsI BclI Bme1580I BmrI BpuEI BsaI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI
BstEII BstYI BtgI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I HindIII MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PciI PfoI PpuMI
PstI PvuII SacI SfcI SmlI SphI StyI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpmI Bpu10I
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI ZraI