Restriction map for
pF1KB7034
(Linear) (Six-base) MAPSORT of: KIBB7034.seq Check: 262 from: 1 to: 1275
KIBB7034.orf
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AccI GT'mk_AC
Cuts at: 0 1145 1275
Size: 1145 130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1275
Size: 21 1254
AflIII A'CryG_T
Cuts at: 0 554 1275
Size: 554 721
AgeI A'CCGG_T
Cuts at: 0 596 1275
Size: 596 679
AleI CACnn'nnGTG
Cuts at: 0 331 1275
Size: 331 944
AlwNI CAG_nnn'CTG
Cuts at: 0 296 949 1042 1275
Size: 296 653 93 233
Fragments arranged by size:
653 296 233 93
ApaI G_GGCC'C
Cuts at: 0 78 1275
Size: 78 1197
AvaI C'yCGr_G
Cuts at: 0 71 739 926 1275
Size: 71 668 187 349
Fragments arranged by size:
668 349 187 71
BanI G'GyrC_C
Cuts at: 0 735 1275
Size: 735 540
BanII G_rGCy'C
Cuts at: 0 78 746 1275
Size: 78 668 529
Fragments arranged by size:
668 529 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 822 1275
Size: 822 453
BciVI GTATCCnnnnn_n'
Cuts at: 0 626 1218 1275
Size: 626 592 57
Fragments arranged by size:
626 592 57
BclI T'GATC_A
Cuts at: 0 848 1275
Size: 848 427
BglI GCCn_nnn'nGGC
Cuts at: 0 12 679 1275
Size: 12 667 596
Fragments arranged by size:
667 596 12
Bme1580I G_kGCm'C
Cuts at: 0 78 740 1275
Size: 78 662 535
Fragments arranged by size:
662 535 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 1275
Size: 700 575
BsaWI w'CCGG_w
Cuts at: 0 277 328 596 1275
Size: 277 51 268 679
Fragments arranged by size:
679 277 268 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 323 353 1275
Size: 323 30 922
Fragments arranged by size:
922 323 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 703 1051 1275
Size: 190 513 348 224
Fragments arranged by size:
513 348 224 190
BseYI C'CCAG_C
Cuts at: 0 1180 1275
Size: 1180 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1275
Size: 409 866
BsiHKAI G_wGCw'C
Cuts at: 0 464 773 1275
Size: 464 309 502
Fragments arranged by size:
502 464 309
BsmI GAATG_Cn'
Cuts at: 0 256 1275
Size: 256 1019
Bsp1286I G_dGCh'C
Cuts at: 0 78 464 740 746 773 1275
Size: 78 386 276 6 27 502
Fragments arranged by size:
502 386 276 78 27 6
BspEI T'CCGG_A
Cuts at: 0 277 1275
Size: 277 998
BspHI T'CATG_A
Cuts at: 0 575 762 1275
Size: 575 187 513
Fragments arranged by size:
575 513 187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1237 1275
Size: 1237 38
BsrFI r'CCGG_y
Cuts at: 0 405 596 1275
Size: 405 191 679
Fragments arranged by size:
679 405 191
BsrGI T'GTAC_A
Cuts at: 0 323 416 1275
Size: 323 93 859
Fragments arranged by size:
859 323 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 103 1088 1275
Size: 103 985 187
Fragments arranged by size:
985 187 103
BstYI r'GATC_y
Cuts at: 0 114 1275
Size: 114 1161
BtgI C'CryG_G
Cuts at: 0 7 434 857 1275
Size: 7 427 423 418
Fragments arranged by size:
427 423 418 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 675 1275
Size: 675 600
BtsI GCAGTG_nn'
Cuts at: 0 501 533 1203 1275
Size: 501 32 670 72
Fragments arranged by size:
670 501 72 32
EaeI y'GGCC_r
Cuts at: 0 26 365 431 671 686 1275
Size: 26 339 66 240 15 589
Fragments arranged by size:
589 339 240 66 26 15
EarI CTCTTCn'nnn_
Cuts at: 0 242 900 1275
Size: 242 658 375
Fragments arranged by size:
658 375 242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 1275
Size: 21 1254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 1216 1248 1275
Size: 106 32 1078 32 27
Fragments arranged by size:
1078 106 32 32 27
HincII GTy'rAC
Cuts at: 0 197 1271 1275
Size: 197 1074 4
Fragments arranged by size:
1074 197 4
HpaI GTT'AAC
Cuts at: 0 197 1271 1275
Size: 197 1074 4
Fragments arranged by size:
1074 197 4
MscI TGG'CCA
Cuts at: 0 367 433 673 688 1275
Size: 367 66 240 15 587
Fragments arranged by size:
587 367 240 66 15
MslI CAynn'nnrTG
Cuts at: 0 331 373 733 1275
Size: 331 42 360 542
Fragments arranged by size:
542 360 331 42
MspA1I CmG'CkG
Cuts at: 0 9 54 846 946 1275
Size: 9 45 792 100 329
Fragments arranged by size:
792 329 100 45 9
NaeI GCC'GGC
Cuts at: 0 407 1275
Size: 407 868
NgoMIV G'CCGG_C
Cuts at: 0 405 1275
Size: 405 870
NspI r_CATG'y
Cuts at: 0 423 558 1275
Size: 423 135 717
Fragments arranged by size:
717 423 135
PciI A'CATG_T
Cuts at: 0 554 1275
Size: 554 721
PflMI CCAn_nnn'nTGG
Cuts at: 0 296 1275
Size: 296 979
PsiI TTA'TAA
Cuts at: 0 1190 1275
Size: 1190 85
PspOMI G'GGCC_C
Cuts at: 0 74 1275
Size: 74 1201
PstI C_TGCA'G
Cuts at: 0 540 1275
Size: 540 735
PvuII CAG'CTG
Cuts at: 0 946 1275
Size: 946 329
SacII CC_GC'GG
Cuts at: 0 10 1275
Size: 10 1265
SapI GCTCTTCn'nnn_
Cuts at: 0 900 1275
Size: 900 375
SfcI C'TryA_G
Cuts at: 0 536 1146 1275
Size: 536 610 129
Fragments arranged by size:
610 536 129
SmaI CCC'GGG
Cuts at: 0 73 741 928 1275
Size: 73 668 187 347
Fragments arranged by size:
668 347 187 73
SmlI C'TyrA_G
Cuts at: 0 715 1275
Size: 715 560
SrfI GCCC'GGGC
Cuts at: 0 741 1275
Size: 741 534
TatI w'GTAC_w
Cuts at: 0 323 416 1275
Size: 323 93 859
Fragments arranged by size:
859 323 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 99 131 296 328 1275
Size: 99 32 165 32 947
Fragments arranged by size:
947 165 99 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 1275
Size: 681 594
XmaI C'CCGG_G
Cuts at: 0 71 739 926 1275
Size: 71 668 187 349
Fragments arranged by size:
668 349 187 71
XmnI GAAnn'nnTTC
Cuts at: 0 654 1275
Size: 654 621
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlwNI ApaI AvaI
BanI BanII BbsI BciVI BclI BglI Bme1580I BpuEI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrFI BsrGI BstAPI BstYI BtgI
BtgZI BtsI EaeI EarI Eco57MI Hin4I HincII HpaI
MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI
PsiI PspOMI PstI PvuII SacII SapI SfcI SmaI
SmlI SrfI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacI
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII Tth111I XbaI XhoI ZraI