Restriction map for
pF1KB7035
(Linear) (Six-base) MAPSORT of: KIBB7035.seq Check: 4037 from: 1 to: 1278
KIBB7035.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 632 1278
Size: 632 646
Acc65I G'GTAC_C
Cuts at: 0 231 1278
Size: 231 1047
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1278
Size: 920 358
AhdI GACnn_n'nnGTC
Cuts at: 0 105 217 1278
Size: 105 112 1061
Fragments arranged by size:
1061 112 105
AvaI C'yCGr_G
Cuts at: 0 1062 1278
Size: 1062 216
BamHI G'GATC_C
Cuts at: 0 73 1278
Size: 73 1205
BanI G'GyrC_C
Cuts at: 0 231 751 1278
Size: 231 520 527
Fragments arranged by size:
527 520 231
BanII G_rGCy'C
Cuts at: 0 709 1278
Size: 709 569
BbeI G_GCGC'C
Cuts at: 0 755 1278
Size: 755 523
BbsI GAAGACnn'nnnn_
Cuts at: 0 406 651 908 1278
Size: 406 245 257 370
Fragments arranged by size:
406 370 257 245
Bme1580I G_kGCm'C
Cuts at: 0 86 1063 1178 1278
Size: 86 977 115 100
Fragments arranged by size:
977 115 100 86
BmgBI CAC'GTC
Cuts at: 0 1082 1278
Size: 1082 196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 375 1278
Size: 375 903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1278
Size: 654 624
BsaI GGTCTCn'nnnn_
Cuts at: 0 204 1182 1278
Size: 204 978 96
Fragments arranged by size:
978 204 96
BsaHI Gr'CG_yC
Cuts at: 0 752 921 1278
Size: 752 169 357
Fragments arranged by size:
752 357 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 1255 1278
Size: 280 30 945 23
Fragments arranged by size:
945 280 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 69 615 808 1276 1278
Size: 69 546 193 468 2
Fragments arranged by size:
546 468 193 69 2
BseYI C'CCAG_C
Cuts at: 0 224 1278
Size: 224 1054
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 391 476 1278
Size: 181 210 85 802
Fragments arranged by size:
802 210 181 85
BsiHKAI G_wGCw'C
Cuts at: 0 649 709 1276 1278
Size: 649 60 567 2
Fragments arranged by size:
649 567 60 2
Bsp1286I G_dGCh'C
Cuts at: 0 86 649 709 1063 1178 1276 1278
Size: 86 563 60 354 115 98 2
Fragments arranged by size:
563 354 115 98 86 60 2
BsrDI GCAATG_nn'
Cuts at: 0 1016 1278
Size: 1016 262
BsrFI r'CCGG_y
Cuts at: 0 228 917 1278
Size: 228 689 361
Fragments arranged by size:
689 361 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 748 1278
Size: 748 530
BstYI r'GATC_y
Cuts at: 0 73 1278
Size: 73 1205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 898 1278
Size: 898 380
BtsI GCAGTG_nn'
Cuts at: 0 438 742 1278
Size: 438 304 536
Fragments arranged by size:
536 438 304
DraI TTT'AAA
Cuts at: 0 188 1278
Size: 188 1090
EaeI y'GGCC_r
Cuts at: 0 119 1278
Size: 119 1159
EarI CTCTTCn'nnn_
Cuts at: 0 202 475 529 563 637 785 1278
Size: 202 273 54 34 74 148 493
Fragments arranged by size:
493 273 202 148 74 54 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 508 833 1278
Size: 508 325 445
Fragments arranged by size:
508 445 325
EcoICRI GAG'CTC
Cuts at: 0 707 1278
Size: 707 571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 920 1278
Size: 654 266 358
Fragments arranged by size:
654 358 266
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 1278
Size: 130 1148
EcoO109I rG'GnC_Cy
Cuts at: 0 1088 1278
Size: 1088 190
HaeII r_GCGC'y
Cuts at: 0 247 755 1278
Size: 247 508 523
Fragments arranged by size:
523 508 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 203 235 860 892 1005 1037
Size: 91 32 80 32 625 32 113 32
Cuts at: 1037 1278
Size: 241
Fragments arranged by size:
625 241 113 91 80 32 32 32
32
HincII GTy'rAC
Cuts at: 0 954 1278
Size: 954 324
HindIII A'AGCT_T
Cuts at: 0 265 1278
Size: 265 1013
KasI G'GCGC_C
Cuts at: 0 751 1278
Size: 751 527
KpnI G_GTAC'C
Cuts at: 0 235 1278
Size: 235 1043
MslI CAynn'nnrTG
Cuts at: 0 1198 1255 1278
Size: 1198 57 23
Fragments arranged by size:
1198 57 23
MspA1I CmG'CkG
Cuts at: 0 684 1278
Size: 684 594
NaeI GCC'GGC
Cuts at: 0 919 1278
Size: 919 359
NarI GG'CG_CC
Cuts at: 0 752 1278
Size: 752 526
NgoMIV G'CCGG_C
Cuts at: 0 917 1278
Size: 917 361
NspI r_CATG'y
Cuts at: 0 195 658 1134 1278
Size: 195 463 476 144
Fragments arranged by size:
476 463 195 144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 599 631 1278
Size: 599 32 647
Fragments arranged by size:
647 599 32
PpuMI rG'GwC_Cy
Cuts at: 0 1088 1278
Size: 1088 190
PshAI GACnn'nnGTC
Cuts at: 0 508 1194 1278
Size: 508 686 84
Fragments arranged by size:
686 508 84
PstI C_TGCA'G
Cuts at: 0 887 1278
Size: 887 391
SacI G_AGCT'C
Cuts at: 0 709 1278
Size: 709 569
SapI GCTCTTCn'nnn_
Cuts at: 0 475 637 1278
Size: 475 162 641
Fragments arranged by size:
641 475 162
SfcI C'TryA_G
Cuts at: 0 883 1278
Size: 883 395
SfoI GGC'GCC
Cuts at: 0 753 1278
Size: 753 525
SgrAI Cr'CCGG_yG
Cuts at: 0 917 1278
Size: 917 361
SphI G_CATG'C
Cuts at: 0 1134 1278
Size: 1134 144
StyI C'CwwG_G
Cuts at: 0 236 422 485 1278
Size: 236 186 63 793
Fragments arranged by size:
793 236 186 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 1259 1278
Size: 238 1021 19
Fragments arranged by size:
1021 238 19
TatI w'GTAC_w
Cuts at: 0 1205 1278
Size: 1205 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 714 746 1085 1117 1255 1278
Size: 714 32 339 32 138 23
Fragments arranged by size:
714 339 138 32 32 23
XmnI GAAnn'nnTTC
Cuts at: 0 973 1278
Size: 973 305
Enzymes that do cut:
AccI Acc65I AcuI AhdI AvaI BamHI BanI BanII
BbeI BbsI Bme1580I BmgBI BplI BpmI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI
BstXI BstYI BtgZI BtsI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
KasI KpnI MslI MspA1I NaeI NarI NgoMIV NspI
PpiI PpuMI PshAI PstI SacI SapI SfcI SfoI
SgrAI SphI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HpaI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI