Restriction map for
pF1KB7043
(Linear) (Six-base) MAPSORT of: KIBB7043.seq Check: 3739 from: 1 to: 1410
KIBB7043.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1329 1410
Size: 1329 81
AhdI GACnn_n'nnGTC
Cuts at: 0 302 1410
Size: 302 1108
AlwNI CAG_nnn'CTG
Cuts at: 0 831 849 1410
Size: 831 18 561
Fragments arranged by size:
831 561 18
ApaI G_GGCC'C
Cuts at: 0 699 1265 1410
Size: 699 566 145
Fragments arranged by size:
699 566 145
AvaI C'yCGr_G
Cuts at: 0 139 289 337 487 1410
Size: 139 150 48 150 923
Fragments arranged by size:
923 150 150 139 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 370 403 1104 1137 1410
Size: 370 33 701 33 273
Fragments arranged by size:
701 370 273 33 33
BanI G'GyrC_C
Cuts at: 0 276 285 1029 1053 1410
Size: 276 9 744 24 357
Fragments arranged by size:
744 357 276 24 9
BanII G_rGCy'C
Cuts at: 0 140 699 941 1265 1410
Size: 140 559 242 324 145
Fragments arranged by size:
559 324 242 145 140
BbeI G_GCGC'C
Cuts at: 0 280 289 1410
Size: 280 9 1121
Fragments arranged by size:
1121 280 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 617 796 1410
Size: 416 201 179 614
Fragments arranged by size:
614 416 201 179
BbvCI CC'TCA_GC
Cuts at: 0 774 1410
Size: 774 636
Bme1580I G_kGCm'C
Cuts at: 0 650 699 1010 1058 1265 1410
Size: 650 49 311 48 207 145
Fragments arranged by size:
650 311 207 145 49 48
BmgBI CAC'GTC
Cuts at: 0 1170 1410
Size: 1170 240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 789 1410
Size: 789 621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1125 1192 1410
Size: 71 1054 67 218
Fragments arranged by size:
1054 218 71 67
Bpu10I CC'TnA_GC
Cuts at: 0 774 1410
Size: 774 636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 910 1126 1410
Size: 727 183 216 284
Fragments arranged by size:
727 284 216 183
BsaI GGTCTCn'nnnn_
Cuts at: 0 973 1410
Size: 973 437
BsaAI yAC'GTr
Cuts at: 0 471 1410
Size: 471 939
BsaHI Gr'CG_yC
Cuts at: 0 277 286 874 1410
Size: 277 9 588 536
Fragments arranged by size:
588 536 277 9
BsaWI w'CCGG_w
Cuts at: 0 678 1410
Size: 678 732
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 705 1410
Size: 697 8 705
Fragments arranged by size:
705 697 8
BseYI C'CCAG_C
Cuts at: 0 162 300 311 869 940 1410
Size: 162 138 11 558 71 470
Fragments arranged by size:
558 470 162 138 71 11
BsiEI CG_ry'CG
Cuts at: 0 691 733 1410
Size: 691 42 677
Fragments arranged by size:
691 677 42
BsiHKAI G_wGCw'C
Cuts at: 0 658 1157 1410
Size: 658 499 253
Fragments arranged by size:
658 499 253
BsiWI C'GTAC_G
Cuts at: 0 269 1410
Size: 269 1141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 1410
Size: 85 1325
Bsp1286I G_dGCh'C
Cuts at: 0 140 650 658 699 941 1010 1058 1157
Size: 140 510 8 41 242 69 48 99
Cuts at: 1157 1265 1410
Size: 108 145
Fragments arranged by size:
510 242 145 140 108 99 69 48
41 8
BspEI T'CCGG_A
Cuts at: 0 678 1410
Size: 678 732
BsrBI CCG'CTC
Cuts at: 0 352 521 687 1410
Size: 352 169 166 723
Fragments arranged by size:
723 352 169 166
BsrDI GCAATG_nn'
Cuts at: 0 1328 1410
Size: 1328 82
BsrFI r'CCGG_y
Cuts at: 0 732 1035 1410
Size: 732 303 375
Fragments arranged by size:
732 375 303
BsrGI T'GTAC_A
Cuts at: 0 1399 1410
Size: 1399 11
BssHII G'CGCG_C
Cuts at: 0 69 1410
Size: 69 1341
BssSI C'ACGA_G
Cuts at: 0 651 1410
Size: 651 759
BstAPI GCAn_nnn'nTGC
Cuts at: 0 852 1410
Size: 852 558
BstYI r'GATC_y
Cuts at: 0 956 1410
Size: 956 454
Bsu36I CC'TnA_GG
Cuts at: 0 80 1410
Size: 80 1330
BtgI C'CryG_G
Cuts at: 0 220 374 418 575 1410
Size: 220 154 44 157 835
Fragments arranged by size:
835 220 157 154 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 146 1410
Size: 146 1264
BtsI GCAGTG_nn'
Cuts at: 0 997 1189 1410
Size: 997 192 221
Fragments arranged by size:
997 221 192
EaeI y'GGCC_r
Cuts at: 0 496 688 730 1410
Size: 496 192 42 680
Fragments arranged by size:
680 496 192 42
EagI C'GGCC_G
Cuts at: 0 688 730 1410
Size: 688 42 680
Fragments arranged by size:
688 680 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 186 1050 1054 1410
Size: 186 864 4 356
Fragments arranged by size:
864 356 186 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1125 1192 1329 1410
Size: 71 1054 67 137 81
Fragments arranged by size:
1054 137 81 71 67
EcoO109I rG'GnC_Cy
Cuts at: 0 695 696 1261 1262 1410
Size: 695 1 565 1 148
Fragments arranged by size:
695 565 148 1 1
FspI TGC'GCA
Cuts at: 0 862 1410
Size: 862 548
HaeII r_GCGC'y
Cuts at: 0 184 280 289 487 559 583 742 1410
Size: 184 96 9 198 72 24 159 668
Fragments arranged by size:
668 198 184 159 96 72 24 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 288 320 645 677 700 732 1410
Size: 288 32 325 32 23 32 678
Fragments arranged by size:
678 325 288 32 32 32 23
KasI G'GCGC_C
Cuts at: 0 276 285 1410
Size: 276 9 1125
Fragments arranged by size:
1125 276 9
MslI CAynn'nnrTG
Cuts at: 0 49 1246 1410
Size: 49 1197 164
Fragments arranged by size:
1197 164 49
MspA1I CmG'CkG
Cuts at: 0 194 392 420 593 778 849 1410
Size: 194 198 28 173 185 71 561
Fragments arranged by size:
561 198 194 185 173 71 28
NaeI GCC'GGC
Cuts at: 0 1037 1410
Size: 1037 373
NarI GG'CG_CC
Cuts at: 0 277 286 1410
Size: 277 9 1124
Fragments arranged by size:
1124 277 9
NgoMIV G'CCGG_C
Cuts at: 0 1035 1410
Size: 1035 375
NspI r_CATG'y
Cuts at: 0 48 249 948 1410
Size: 48 201 699 462
Fragments arranged by size:
699 462 201 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 849 1103 1410
Size: 849 254 307
Fragments arranged by size:
849 307 254
PmlI CAC'GTG
Cuts at: 0 471 1410
Size: 471 939
PspOMI G'GGCC_C
Cuts at: 0 695 1261 1410
Size: 695 566 149
Fragments arranged by size:
695 566 149
PstI C_TGCA'G
Cuts at: 0 953 1019 1184 1410
Size: 953 66 165 226
Fragments arranged by size:
953 226 165 66
PvuII CAG'CTG
Cuts at: 0 849 1410
Size: 849 561
SacII CC_GC'GG
Cuts at: 0 421 1410
Size: 421 989
ScaI AGT'ACT
Cuts at: 0 811 1410
Size: 811 599
SfcI C'TryA_G
Cuts at: 0 240 438 949 1015 1180 1410
Size: 240 198 511 66 165 230
Fragments arranged by size:
511 240 230 198 165 66
SfoI GGC'GCC
Cuts at: 0 278 287 1410
Size: 278 9 1123
Fragments arranged by size:
1123 278 9
SgrAI Cr'CCGG_yG
Cuts at: 0 1035 1410
Size: 1035 375
SmaI CCC'GGG
Cuts at: 0 141 339 1410
Size: 141 198 1071
Fragments arranged by size:
1071 198 141
SmlI C'TyrA_G
Cuts at: 0 742 889 1141 1410
Size: 742 147 252 269
Fragments arranged by size:
742 269 252 147
SphI G_CATG'C
Cuts at: 0 249 948 1410
Size: 249 699 462
Fragments arranged by size:
699 462 249
StyI C'CwwG_G
Cuts at: 0 1124 1257 1410
Size: 1124 133 153
Fragments arranged by size:
1124 153 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 233 1410
Size: 233 1177
TatI w'GTAC_w
Cuts at: 0 809 1193 1399 1410
Size: 809 384 206 11
Fragments arranged by size:
809 384 206 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 506 1410
Size: 506 904
XmaI C'CCGG_G
Cuts at: 0 139 337 1410
Size: 139 198 1073
Fragments arranged by size:
1073 198 139
Enzymes that do cut:
AcuI AhdI AlwNI ApaI AvaI BaeI BanI BanII
BbeI BbsI BbvCI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EagI EciI Eco57MI EcoO109I FspI HaeII
Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI
PflMI PmlI PspOMI PstI PvuII SacII ScaI SfcI
SfoI SgrAI SmaI SmlI SphI StyI TaqII TatI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BsaBI BsaXI BsgI BsmI
BspHI BspMI BstBI BstEII BstXI BstZ17I ClaI CspCI
DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI SexAI SfiI SgfI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmnI ZraI