Restriction map for
pF1KB7055
(Linear) (Six-base) MAPSORT of: KIBB7055.seq Check: 1301 from: 1 to: 1746
KIBB7055.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AatII G_ACGT'C
Cuts at: 0 1251 1746
Size: 1251 495
AccI GT'mk_AC
Cuts at: 0 212 1746
Size: 212 1534
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 614 974 1154 1334 1746
Size: 512 102 360 180 180 412
Fragments arranged by size:
512 412 360 180 180 102
AleI CACnn'nnGTG
Cuts at: 0 299 1746
Size: 299 1447
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1703 1746
Size: 1703 43
AlwNI CAG_nnn'CTG
Cuts at: 0 535 1746
Size: 535 1211
ApaLI G'TGCA_C
Cuts at: 0 539 1746
Size: 539 1207
ApoI r'AATT_y
Cuts at: 0 633 1172 1746
Size: 633 539 574
Fragments arranged by size:
633 574 539
AvrII C'CTAG_G
Cuts at: 0 297 1746
Size: 297 1449
BamHI G'GATC_C
Cuts at: 0 565 1746
Size: 565 1181
BanII G_rGCy'C
Cuts at: 0 779 1746
Size: 779 967
BbsI GAAGACnn'nnnn_
Cuts at: 0 860 1730 1746
Size: 860 870 16
Fragments arranged by size:
870 860 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 936 1116 1296 1476 1746
Size: 936 180 180 180 270
Fragments arranged by size:
936 270 180 180 180
BclI T'GATC_A
Cuts at: 0 222 1698 1746
Size: 222 1476 48
Fragments arranged by size:
1476 222 48
Bme1580I G_kGCm'C
Cuts at: 0 543 1746
Size: 543 1203
BmgBI CAC'GTC
Cuts at: 0 675 1746
Size: 675 1071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1172 1746
Size: 413 759 574
Fragments arranged by size:
759 574 413
BsaI GGTCTCn'nnnn_
Cuts at: 0 335 1746
Size: 335 1411
BsaAI yAC'GTr
Cuts at: 0 874 1746
Size: 874 872
BsaHI Gr'CG_yC
Cuts at: 0 1248 1746
Size: 1248 498
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1746
Size: 704 1042
BsiHKAI G_wGCw'C
Cuts at: 0 543 779 1453 1746
Size: 543 236 674 293
Fragments arranged by size:
674 543 293 236
BsmI GAATG_Cn'
Cuts at: 0 267 1746
Size: 267 1479
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1255 1746
Size: 1255 491
Bsp1286I G_dGCh'C
Cuts at: 0 543 779 1453 1746
Size: 543 236 674 293
Fragments arranged by size:
674 543 293 236
BspHI T'CATG_A
Cuts at: 0 225 1617 1746
Size: 225 1392 129
Fragments arranged by size:
1392 225 129
BsrGI T'GTAC_A
Cuts at: 0 912 1272 1746
Size: 912 360 474
Fragments arranged by size:
912 474 360
BstYI r'GATC_y
Cuts at: 0 103 565 1746
Size: 103 462 1181
Fragments arranged by size:
1181 462 103
BtgI C'CryG_G
Cuts at: 0 897 1746
Size: 897 849
DraIII CAC_nnn'GTG
Cuts at: 0 562 1746
Size: 562 1184
EaeI y'GGCC_r
Cuts at: 0 837 1017 1197 1377 1557 1746
Size: 837 180 180 180 180 189
Fragments arranged by size:
837 189 180 180 180 180
EarI CTCTTCn'nnn_
Cuts at: 0 950 1130 1310 1746
Size: 950 180 180 436
Fragments arranged by size:
950 436 180 180
EcoICRI GAG'CTC
Cuts at: 0 777 1746
Size: 777 969
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 614 974 1154 1334 1746
Size: 512 102 360 180 180 412
Fragments arranged by size:
512 412 360 180 180 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 1746
Size: 271 1475
EcoRI G'AATT_C
Cuts at: 0 1172 1746
Size: 1172 574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 122 1746
Size: 122 1624
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 405 437 798 830 1518 1537 1550 1569
Size: 405 32 361 32 688 19 13 19
Cuts at: 1569 1746
Size: 177
Fragments arranged by size:
688 405 361 177 32 32 19 19
13
HincII GTy'rAC
Cuts at: 0 310 609 672 1746
Size: 310 299 63 1074
Fragments arranged by size:
1074 310 299 63
HindIII A'AGCT_T
Cuts at: 0 524 1746
Size: 524 1222
HpaI GTT'AAC
Cuts at: 0 609 1746
Size: 609 1137
MfeI C'AATT_G
Cuts at: 0 91 1746
Size: 91 1655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1746
Size: 762 984
MscI TGG'CCA
Cuts at: 0 1019 1199 1379 1559 1746
Size: 1019 180 180 180 187
Fragments arranged by size:
1019 187 180 180 180
MslI CAynn'nnrTG
Cuts at: 0 299 1746
Size: 299 1447
MspA1I CmG'CkG
Cuts at: 0 58 1746
Size: 58 1688
NcoI C'CATG_G
Cuts at: 0 897 1746
Size: 897 849
PflMI CCAn_nnn'nTGG
Cuts at: 0 666 1746
Size: 666 1080
PsiI TTA'TAA
Cuts at: 0 326 1746
Size: 326 1420
PstI C_TGCA'G
Cuts at: 0 1593 1746
Size: 1593 153
PvuII CAG'CTG
Cuts at: 0 58 1746
Size: 58 1688
SacI G_AGCT'C
Cuts at: 0 779 1746
Size: 779 967
SapI GCTCTTCn'nnn_
Cuts at: 0 1130 1746
Size: 1130 616
SexAI A'CCwGG_T
Cuts at: 0 983 1163 1343 1746
Size: 983 180 180 403
Fragments arranged by size:
983 403 180 180
SfcI C'TryA_G
Cuts at: 0 1589 1746
Size: 1589 157
SmlI C'TyrA_G
Cuts at: 0 428 1187 1746
Size: 428 759 559
Fragments arranged by size:
759 559 428
SpeI A'CTAG_T
Cuts at: 0 600 1746
Size: 600 1146
SspI AAT'ATT
Cuts at: 0 1665 1746
Size: 1665 81
StyI C'CwwG_G
Cuts at: 0 297 897 1746
Size: 297 600 849
Fragments arranged by size:
849 600 297
TatI w'GTAC_w
Cuts at: 0 912 1054 1234 1272 1414 1746
Size: 912 142 180 38 142 332
Fragments arranged by size:
912 332 180 142 142 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 118 142 406 1746
Size: 118 24 264 1340
Fragments arranged by size:
1340 264 118 24
ZraI GAC'GTC
Cuts at: 0 1249 1746
Size: 1249 497
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AlwNI ApaLI ApoI
AvrII BamHI BanII BbsI BciVI BclI Bme1580I BmgBI
BpuEI BsaI BsaAI BsaHI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrGI BstYI BtgI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI PflMI
PsiI PstI PvuII SacI SapI SexAI SfcI SmlI
SpeI SspI StyI TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvaI BaeI BanI
BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmrI
BmtI BplI BpmI Bpu10I BsaBI BsaWI BsaXI BseRI
BseYI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EciI
EcoO109I EcoRV FseI FspI FspAI HaeII KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI