Restriction map for
pF1KB7057
(Linear) (Six-base) MAPSORT of: KIBB7057.seq Check: 7456 from: 1 to: 1815
KIBB7057.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 1128 1352 1815
Size: 386 742 224 463
Fragments arranged by size:
742 463 386 224
AatII G_ACGT'C
Cuts at: 0 1466 1815
Size: 1466 349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 814 1815
Size: 624 190 1001
Fragments arranged by size:
1001 624 190
AflIII A'CryG_T
Cuts at: 0 707 851 1815
Size: 707 144 964
Fragments arranged by size:
964 707 144
AleI CACnn'nnGTG
Cuts at: 0 1087 1815
Size: 1087 728
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 838 870 1126 1158 1815
Size: 838 32 256 32 657
Fragments arranged by size:
838 657 256 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 351 515 1287 1368 1815
Size: 351 164 772 81 447
Fragments arranged by size:
772 447 351 164 81
ApaI G_GGCC'C
Cuts at: 0 64 115 1438 1815
Size: 64 51 1323 377
Fragments arranged by size:
1323 377 64 51
AvaI C'yCGr_G
Cuts at: 0 216 774 1227 1575 1759 1815
Size: 216 558 453 348 184 56
Fragments arranged by size:
558 453 348 216 184 56
BanI G'GyrC_C
Cuts at: 0 223 778 1506 1815
Size: 223 555 728 309
Fragments arranged by size:
728 555 309 223
BanII G_rGCy'C
Cuts at: 0 64 115 167 494 659 1235 1316 1438
Size: 64 51 52 327 165 576 81 122
Cuts at: 1438 1775 1815
Size: 337 40
Fragments arranged by size:
576 337 327 165 122 81 64 52
51 40
BbeI G_GCGC'C
Cuts at: 0 782 1815
Size: 782 1033
BbsI GAAGACnn'nnnn_
Cuts at: 0 165 1815
Size: 165 1650
BbvCI CC'TCA_GC
Cuts at: 0 457 1531 1789 1815
Size: 457 1074 258 26
Fragments arranged by size:
1074 457 258 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 741 775 1815
Size: 741 34 1040
Fragments arranged by size:
1040 741 34
BglI GCCn_nnn'nGGC
Cuts at: 0 329 700 1268 1815
Size: 329 371 568 547
Fragments arranged by size:
568 547 371 329
BlpI GC'TnA_GC
Cuts at: 0 166 989 1815
Size: 166 823 826
Fragments arranged by size:
826 823 166
Bme1580I G_kGCm'C
Cuts at: 0 64 115 124 1438 1676 1815
Size: 64 51 9 1314 238 139
Fragments arranged by size:
1314 238 139 64 51 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 1522 1658 1815
Size: 67 1455 136 157
Fragments arranged by size:
1455 157 136 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 168 276 358 406 502 715 865
Size: 94 74 108 82 48 96 213 150
Cuts at: 865 967 1033 1198 1330 1399 1501 1603 1618
Size: 102 66 165 132 69 102 102 15
Cuts at: 1618 1815
Size: 197
Fragments arranged by size:
213 197 165 150 132 108 102 102
102 96 94 82 74 69 66 48
15
Bpu10I CC'TnA_GC
Cuts at: 0 457 673 1531 1789 1815
Size: 457 216 858 258 26
Fragments arranged by size:
858 457 258 216 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1234 1615 1815
Size: 1039 195 381 200
Fragments arranged by size:
1039 381 200 195
BsaHI Gr'CG_yC
Cuts at: 0 779 1427 1463 1815
Size: 779 648 36 352
Fragments arranged by size:
779 648 352 36
BsaWI w'CCGG_w
Cuts at: 0 1121 1131 1815
Size: 1121 10 684
Fragments arranged by size:
1121 684 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 838 868 1126 1156 1309 1339 1576 1606
Size: 838 30 258 30 153 30 237 30
Cuts at: 1606 1815
Size: 209
Fragments arranged by size:
838 258 237 209 153 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 964 1291 1815
Size: 964 327 524
Fragments arranged by size:
964 524 327
BseYI C'CCAG_C
Cuts at: 0 114 327 354 867 960 1815
Size: 114 213 27 513 93 855
Fragments arranged by size:
855 513 213 114 93 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 736 922 1147 1156 1815
Size: 364 372 186 225 9 659
Fragments arranged by size:
659 372 364 225 186 9
BsiEI CG_ry'CG
Cuts at: 0 128 1815
Size: 128 1687
BsiHKAI G_wGCw'C
Cuts at: 0 167 554 659 1028 1316 1815
Size: 167 387 105 369 288 499
Fragments arranged by size:
499 387 369 288 167 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1470 1815
Size: 1470 345
Bsp1286I G_dGCh'C
Cuts at: 0 64 115 124 167 494 554 659 1028
Size: 64 51 9 43 327 60 105 369
Cuts at: 1028 1235 1316 1438 1676 1775 1815
Size: 207 81 122 238 99 40
Fragments arranged by size:
369 327 238 207 122 105 99 81
64 60 51 43 40 9
BspEI T'CCGG_A
Cuts at: 0 1121 1131 1815
Size: 1121 10 684
Fragments arranged by size:
1121 684 10
BspHI T'CATG_A
Cuts at: 0 1097 1815
Size: 1097 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 386 632 944 1128 1352 1394 1787
Size: 320 66 246 312 184 224 42 393
Cuts at: 1787 1815
Size: 28
Fragments arranged by size:
393 320 312 246 224 184 66 42
28
BsrBI CCG'CTC
Cuts at: 0 390 1815
Size: 390 1425
BsrDI GCAATG_nn'
Cuts at: 0 1486 1815
Size: 1486 329
BsrFI r'CCGG_y
Cuts at: 0 1219 1419 1815
Size: 1219 200 396
Fragments arranged by size:
1219 396 200
BssSI C'ACGA_G
Cuts at: 0 1264 1815
Size: 1264 551
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1170 1815
Size: 1170 645
BstEII G'GTnAC_C
Cuts at: 0 1603 1815
Size: 1603 212
Bsu36I CC'TnA_GG
Cuts at: 0 7 1537 1815
Size: 7 1530 278
Fragments arranged by size:
1530 278 7
BtgI C'CryG_G
Cuts at: 0 570 767 1815
Size: 570 197 1048
Fragments arranged by size:
1048 570 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 744 819 888 1815
Size: 744 75 69 927
Fragments arranged by size:
927 744 75 69
BtsI GCAGTG_nn'
Cuts at: 0 1549 1815
Size: 1549 266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 217 252 1815
Size: 217 35 1563
Fragments arranged by size:
1563 217 35
EaeI y'GGCC_r
Cuts at: 0 125 321 650 1815
Size: 125 196 329 1165
Fragments arranged by size:
1165 329 196 125
EagI C'GGCC_G
Cuts at: 0 125 1815
Size: 125 1690
EarI CTCTTCn'nnn_
Cuts at: 0 499 643 1249 1363 1815
Size: 499 144 606 114 452
Fragments arranged by size:
606 499 452 144 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1192 1225 1315 1438 1815
Size: 1192 33 90 123 377
Fragments arranged by size:
1192 377 123 90 33
EcoICRI GAG'CTC
Cuts at: 0 165 657 1314 1815
Size: 165 492 657 501
Fragments arranged by size:
657 501 492 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 168 276 358 406 502 624 715
Size: 94 74 108 82 48 96 122 91
Cuts at: 715 814 865 967 1033 1198 1330 1399 1501
Size: 99 51 102 66 165 132 69 102
Cuts at: 1501 1603 1618 1815
Size: 102 15 197
Fragments arranged by size:
197 165 132 122 108 102 102 102
99 96 94 91 82 74 69 66
51 48 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 844 1360 1792 1815
Size: 844 516 432 23
Fragments arranged by size:
844 516 432 23
EcoO109I rG'GnC_Cy
Cuts at: 0 61 111 501 519 933 1005 1149 1365
Size: 61 50 390 18 414 72 144 216
Cuts at: 1365 1435 1785 1815
Size: 70 350 30
Fragments arranged by size:
414 390 350 216 144 72 70 61
50 30 18
FspI TGC'GCA
Cuts at: 0 403 1815
Size: 403 1412
HaeII r_GCGC'y
Cuts at: 0 34 352 782 802 1070 1590 1639 1815
Size: 34 318 430 20 268 520 49 176
Fragments arranged by size:
520 430 318 268 176 49 34 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1232 1264 1309 1341 1815
Size: 1232 32 45 32 474
Fragments arranged by size:
1232 474 45 32 32
KasI G'GCGC_C
Cuts at: 0 778 1815
Size: 778 1037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 1815
Size: 1352 463
MslI CAynn'nnrTG
Cuts at: 0 1087 1815
Size: 1087 728
MspA1I CmG'CkG
Cuts at: 0 327 399 572 644 720 960 984 1187
Size: 327 72 173 72 76 240 24 203
Cuts at: 1187 1446 1815
Size: 259 369
Fragments arranged by size:
369 327 259 240 203 173 76 72
72 24
NaeI GCC'GGC
Cuts at: 0 1421 1815
Size: 1421 394
NarI GG'CG_CC
Cuts at: 0 779 1815
Size: 779 1036
NgoMIV G'CCGG_C
Cuts at: 0 1419 1815
Size: 1419 396
PasI CC'CwG_GG
Cuts at: 0 1431 1610 1815
Size: 1431 179 205
Fragments arranged by size:
1431 205 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 1368 1815
Size: 1368 447
PfoI T'CCnGG_A
Cuts at: 0 184 659 842 944 1049 1815
Size: 184 475 183 102 105 766
Fragments arranged by size:
766 475 184 183 105 102
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 1126 1158 1815
Size: 838 32 256 32 657
Fragments arranged by size:
838 657 256 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 519 933 1785 1815
Size: 519 414 852 30
Fragments arranged by size:
852 519 414 30
PspOMI G'GGCC_C
Cuts at: 0 60 111 1434 1815
Size: 60 51 1323 381
Fragments arranged by size:
1323 381 60 51
PstI C_TGCA'G
Cuts at: 0 142 181 317 629 959 1445 1658 1815
Size: 142 39 136 312 330 486 213 157
Fragments arranged by size:
486 330 312 213 157 142 136 39
PvuII CAG'CTG
Cuts at: 0 327 399 720 960 984 1187 1815
Size: 327 72 321 240 24 203 628
Fragments arranged by size:
628 327 321 240 203 72 24
SacI G_AGCT'C
Cuts at: 0 167 659 1316 1815
Size: 167 492 657 499
Fragments arranged by size:
657 499 492 167
SacII CC_GC'GG
Cuts at: 0 573 1815
Size: 573 1242
SapI GCTCTTCn'nnn_
Cuts at: 0 499 643 1815
Size: 499 144 1172
Fragments arranged by size:
1172 499 144
SbfI CC_TGCA'GG
Cuts at: 0 317 1815
Size: 317 1498
SfcI C'TryA_G
Cuts at: 0 138 177 313 625 955 1441 1654 1815
Size: 138 39 136 312 330 486 213 161
Fragments arranged by size:
486 330 312 213 161 138 136 39
SfoI GGC'GCC
Cuts at: 0 780 1815
Size: 780 1035
SmaI CCC'GGG
Cuts at: 0 218 776 1577 1815
Size: 218 558 801 238
Fragments arranged by size:
801 558 238 218
SmlI C'TyrA_G
Cuts at: 0 1018 1249 1630 1815
Size: 1018 231 381 185
Fragments arranged by size:
1018 381 231 185
StuI AGG'CCT
Cuts at: 0 20 1815
Size: 20 1795
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 241 1815
Size: 39 202 1574
Fragments arranged by size:
1574 202 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 1815
Size: 1576 32 207
Fragments arranged by size:
1576 207 32
Tth111I GACn'n_nGTC
Cuts at: 0 211 1815
Size: 211 1604
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1500 1815
Size: 1500 315
XmaI C'CCGG_G
Cuts at: 0 216 774 1575 1815
Size: 216 558 801 240
Fragments arranged by size:
801 558 240 216
ZraI GAC'GTC
Cuts at: 0 1464 1815
Size: 1464 351
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AloI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV
PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SapI SbfI SfcI SfoI SmaI SmlI
StuI TaqII TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaI
BsaAI BsaBI BsiWI BsmI BsrGI BssHII BstBI BstXI
BstYI BstZ17I ClaI DraI DraIII DrdI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI
SwaI TatI XbaI XhoI XmnI