Restriction map for
pF1KB7058
(Linear) (Six-base) MAPSORT of: KIBB7058.seq Check: 1257 from: 1 to: 1839
KIBB7058.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1387 1839
Size: 1387 452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 760 1650 1839
Size: 421 339 890 189
Fragments arranged by size:
890 421 339 189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1024 1269 1839
Size: 1024 245 570
Fragments arranged by size:
1024 570 245
ApaLI G'TGCA_C
Cuts at: 0 131 1839
Size: 131 1708
ApoI r'AATT_y
Cuts at: 0 180 1839
Size: 180 1659
AvaI C'yCGr_G
Cuts at: 0 9 82 206 273 327 374 642 1430
Size: 9 73 124 67 54 47 268 788
Cuts at: 1430 1839
Size: 409
Fragments arranged by size:
788 409 268 124 73 67 54 47
9
BamHI G'GATC_C
Cuts at: 0 667 1839
Size: 667 1172
BanII G_rGCy'C
Cuts at: 0 9 207 1170 1242 1839
Size: 9 198 963 72 597
Fragments arranged by size:
963 597 198 72 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 517 1715 1839
Size: 517 1198 124
Fragments arranged by size:
1198 517 124
BfrBI ATG'CAT
Cuts at: 0 1712 1839
Size: 1712 127
BglI GCCn_nnn'nGGC
Cuts at: 0 907 1552 1839
Size: 907 645 287
Fragments arranged by size:
907 645 287
BglII A'GATC_T
Cuts at: 0 440 1265 1839
Size: 440 825 574
Fragments arranged by size:
825 574 440
BlpI GC'TnA_GC
Cuts at: 0 1207 1839
Size: 1207 632
Bme1580I G_kGCm'C
Cuts at: 0 135 620 1013 1839
Size: 135 485 393 826
Fragments arranged by size:
826 485 393 135
BmrI ACTGGGnnnn_n'
Cuts at: 0 1630 1839
Size: 1630 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1011 1839
Size: 1011 828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 409 550 1006 1839
Size: 378 31 141 456 833
Fragments arranged by size:
833 456 378 141 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1839
Size: 43 1796
BsaBI GATnn'nnATC
Cuts at: 0 850 1839
Size: 850 989
BsaHI Gr'CG_yC
Cuts at: 0 164 310 1839
Size: 164 146 1529
Fragments arranged by size:
1529 164 146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 463 1839
Size: 235 228 1376
Fragments arranged by size:
1376 235 228
BsiHKAI G_wGCw'C
Cuts at: 0 135 213 375 1062 1170 1242 1839
Size: 135 78 162 687 108 72 597
Fragments arranged by size:
687 597 162 135 108 78 72
BsmI GAATG_Cn'
Cuts at: 0 1712 1714 1839
Size: 1712 2 125
Fragments arranged by size:
1712 125 2
BsmBI CGTCTCn'nnnn_
Cuts at: 0 185 1839
Size: 185 1654
Bsp1286I G_dGCh'C
Cuts at: 0 9 135 207 213 375 620 1013 1062
Size: 9 126 72 6 162 245 393 49
Cuts at: 1062 1170 1242 1839
Size: 108 72 597
Fragments arranged by size:
597 393 245 162 126 108 72 72
49 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 269 1387 1839
Size: 269 1118 452
Fragments arranged by size:
1118 452 269
BsrDI GCAATG_nn'
Cuts at: 0 1833 1839
Size: 1833 6
BsrFI r'CCGG_y
Cuts at: 0 264 725 820 1839
Size: 264 461 95 1019
Fragments arranged by size:
1019 461 264 95
BsrGI T'GTAC_A
Cuts at: 0 1474 1839
Size: 1474 365
BssHII G'CGCG_C
Cuts at: 0 14 1839
Size: 14 1825
BssSI C'ACGA_G
Cuts at: 0 961 1839
Size: 961 878
BstAPI GCAn_nnn'nTGC
Cuts at: 0 857 1839
Size: 857 982
BstEII G'GTnAC_C
Cuts at: 0 1731 1839
Size: 1731 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 609 694 1839
Size: 609 85 1145
Fragments arranged by size:
1145 609 85
BstYI r'GATC_y
Cuts at: 0 440 667 1265 1839
Size: 440 227 598 574
Fragments arranged by size:
598 574 440 227
BtgI C'CryG_G
Cuts at: 0 153 416 803 1763 1839
Size: 153 263 387 960 76
Fragments arranged by size:
960 387 263 153 76
DrdI GACnn_nn'nnGTC
Cuts at: 0 768 1839
Size: 768 1071
EaeI y'GGCC_r
Cuts at: 0 544 599 899 1233 1839
Size: 544 55 300 334 606
Fragments arranged by size:
606 544 334 300 55
EarI CTCTTCn'nnn_
Cuts at: 0 78 436 449 1839
Size: 78 358 13 1390
Fragments arranged by size:
1390 358 78 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 319 1189 1839
Size: 319 870 650
Fragments arranged by size:
870 650 319
EcoICRI GAG'CTC
Cuts at: 0 1168 1240 1839
Size: 1168 72 599
Fragments arranged by size:
1168 599 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 409 421 550 760 1006 1650 1839
Size: 378 31 12 129 210 246 644 189
Fragments arranged by size:
644 378 246 210 189 129 31 12
EcoO109I rG'GnC_Cy
Cuts at: 0 247 258 331 463 700 1839
Size: 247 11 73 132 237 1139
Fragments arranged by size:
1139 247 237 132 73 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 765 1839
Size: 765 1074
HaeII r_GCGC'y
Cuts at: 0 153 476 655 1839
Size: 153 323 179 1184
Fragments arranged by size:
1184 323 179 153
MfeI C'AATT_G
Cuts at: 0 632 1839
Size: 632 1207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 911 1806 1839
Size: 361 550 895 33
Fragments arranged by size:
895 550 361 33
MscI TGG'CCA
Cuts at: 0 546 901 1839
Size: 546 355 938
Fragments arranged by size:
938 546 355
MspA1I CmG'CkG
Cuts at: 0 155 759 948 1206 1839
Size: 155 604 189 258 633
Fragments arranged by size:
633 604 258 189 155
NaeI GCC'GGC
Cuts at: 0 266 1839
Size: 266 1573
NgoMIV G'CCGG_C
Cuts at: 0 264 1839
Size: 264 1575
NsiI A_TGCA'T
Cuts at: 0 1714 1839
Size: 1714 125
NspI r_CATG'y
Cuts at: 0 33 876 1839
Size: 33 843 963
Fragments arranged by size:
963 843 33
PasI CC'CwG_GG
Cuts at: 0 215 1839
Size: 215 1624
PflMI CCAn_nnn'nTGG
Cuts at: 0 977 1839
Size: 977 862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1760 1792 1839
Size: 1760 32 47
Fragments arranged by size:
1760 47 32
PpuMI rG'GwC_Cy
Cuts at: 0 247 258 331 463 700 1839
Size: 247 11 73 132 237 1139
Fragments arranged by size:
1139 247 237 132 73 11
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 736 768 1839
Size: 736 32 1071
Fragments arranged by size:
1071 736 32
PstI C_TGCA'G
Cuts at: 0 294 1839
Size: 294 1545
PvuII CAG'CTG
Cuts at: 0 1206 1839
Size: 1206 633
SacI G_AGCT'C
Cuts at: 0 1170 1242 1839
Size: 1170 72 597
Fragments arranged by size:
1170 597 72
SacII CC_GC'GG
Cuts at: 0 156 1839
Size: 156 1683
SanDI GG'GwC_CC
Cuts at: 0 247 463 700 1839
Size: 247 216 237 1139
Fragments arranged by size:
1139 247 237 216
SapI GCTCTTCn'nnn_
Cuts at: 0 436 1839
Size: 436 1403
SfcI C'TryA_G
Cuts at: 0 137 290 1556 1839
Size: 137 153 1266 283
Fragments arranged by size:
1266 283 153 137
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 907 1839
Size: 907 932
SmaI CCC'GGG
Cuts at: 0 11 275 329 644 1839
Size: 11 264 54 315 1195
Fragments arranged by size:
1195 315 264 54 11
SmlI C'TyrA_G
Cuts at: 0 58 1839
Size: 58 1781
StyI C'CwwG_G
Cuts at: 0 547 671 1839
Size: 547 124 1168
Fragments arranged by size:
1168 547 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 228 664 1149 1839
Size: 228 436 485 690
Fragments arranged by size:
690 485 436 228
TatI w'GTAC_w
Cuts at: 0 1103 1474 1757 1839
Size: 1103 371 283 82
Fragments arranged by size:
1103 371 283 82
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 353 772 894 1839
Size: 353 419 122 945
Fragments arranged by size:
945 419 353 122
XmaI C'CCGG_G
Cuts at: 0 9 273 327 642 1839
Size: 9 264 54 315 1197
Fragments arranged by size:
1197 315 264 54 9
XmnI GAAnn'nnTTC
Cuts at: 0 748 1193 1712 1839
Size: 748 445 519 127
Fragments arranged by size:
748 519 445 127
Enzymes that do cut:
AarI AcuI AlfI ApaLI ApoI AvaI BamHI BanII
BbsI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaBI BsaHI BseRI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstEII BstXI BstYI BtgI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII MfeI MmeI MscI
MspA1I NaeI NgoMIV NsiI NspI PasI PflMI PpiI
PpuMI PsrI PstI PvuII SacI SacII SanDI SapI
SfcI SfiI SmaI SmlI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BclI
BmgBI BmtI Bpu10I BsaI BsaAI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BspEI BspHI BsrBI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EagI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MluI MslI NarI NcoI
NdeI NheI NotI NruI PacI PciI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PvuI RsrII SalI
SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI ZraI