Restriction map for
pF1KB7062
(Linear) (Six-base) MAPSORT of: KIBB7062.seq Check: 4527 from: 1 to: 1992
KIBB7062.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 18 1992
Size: 18 1974
AccI GT'mk_AC
Cuts at: 0 1161 1382 1766 1992
Size: 1161 221 384 226
Fragments arranged by size:
1161 384 226 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 613 991 1988 1992
Size: 257 356 378 997 4
Fragments arranged by size:
997 378 356 257 4
AflII C'TTAA_G
Cuts at: 0 768 1053 1992
Size: 768 285 939
Fragments arranged by size:
939 768 285
AflIII A'CryG_T
Cuts at: 0 239 329 641 1064 1992
Size: 239 90 312 423 928
Fragments arranged by size:
928 423 312 239 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1256 1992
Size: 1256 736
AlwNI CAG_nnn'CTG
Cuts at: 0 1117 1966 1992
Size: 1117 849 26
Fragments arranged by size:
1117 849 26
ApaI G_GGCC'C
Cuts at: 0 17 1992
Size: 17 1975
ApoI r'AATT_y
Cuts at: 0 650 1414 1481 1972 1992
Size: 650 764 67 491 20
Fragments arranged by size:
764 650 491 67 20
AseI AT'TA_AT
Cuts at: 0 460 1123 1586 1992
Size: 460 663 463 406
Fragments arranged by size:
663 463 460 406
BanI G'GyrC_C
Cuts at: 0 81 319 1992
Size: 81 238 1673
Fragments arranged by size:
1673 238 81
BanII G_rGCy'C
Cuts at: 0 17 1992
Size: 17 1975
BbsI GAAGACnn'nnnn_
Cuts at: 0 1555 1992
Size: 1555 437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 662 1992
Size: 628 34 1330
Fragments arranged by size:
1330 628 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 412 1992
Size: 412 1580
BfrBI ATG'CAT
Cuts at: 0 1510 1992
Size: 1510 482
BglII A'GATC_T
Cuts at: 0 161 1992
Size: 161 1831
BlpI GC'TnA_GC
Cuts at: 0 249 1992
Size: 249 1743
Bme1580I G_kGCm'C
Cuts at: 0 17 86 1992
Size: 17 69 1906
Fragments arranged by size:
1906 69 17
BmgBI CAC'GTC
Cuts at: 0 332 1992
Size: 332 1660
BmrI ACTGGGnnnn_n'
Cuts at: 0 883 1022 1992
Size: 883 139 970
Fragments arranged by size:
970 883 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 418 1823 1992
Size: 142 276 1405 169
Fragments arranged by size:
1405 276 169 142
Bpu10I CC'TnA_GC
Cuts at: 0 743 1992
Size: 743 1249
BsaAI yAC'GTr
Cuts at: 0 242 1992
Size: 242 1750
BsaHI Gr'CG_yC
Cuts at: 0 104 963 1992
Size: 104 859 1029
Fragments arranged by size:
1029 859 104
BsiEI CG_ry'CG
Cuts at: 0 8 1992
Size: 8 1984
BsiHKAI G_wGCw'C
Cuts at: 0 158 1992
Size: 158 1834
Bsp1286I G_dGCh'C
Cuts at: 0 17 86 158 1992
Size: 17 69 72 1834
Fragments arranged by size:
1834 72 69 17
BspHI T'CATG_A
Cuts at: 0 1331 1653 1992
Size: 1331 322 339
Fragments arranged by size:
1331 339 322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 39 1992
Size: 18 21 1953
Fragments arranged by size:
1953 21 18
BsrFI r'CCGG_y
Cuts at: 0 140 1992
Size: 140 1852
BsrGI T'GTAC_A
Cuts at: 0 761 1727 1992
Size: 761 966 265
Fragments arranged by size:
966 761 265
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 1992
Size: 30 1962
BstYI r'GATC_y
Cuts at: 0 161 471 1992
Size: 161 310 1521
Fragments arranged by size:
1521 310 161
BstZ17I GTA'TAC
Cuts at: 0 1767 1992
Size: 1767 225
BtgI C'CryG_G
Cuts at: 0 8 1101 1992
Size: 8 1093 891
Fragments arranged by size:
1093 891 8
BtsI GCAGTG_nn'
Cuts at: 0 590 1992
Size: 590 1402
DrdI GACnn_nn'nnGTC
Cuts at: 0 1378 1992
Size: 1378 614
EaeI y'GGCC_r
Cuts at: 0 5 1992
Size: 5 1987
EagI C'GGCC_G
Cuts at: 0 5 1992
Size: 5 1987
EarI CTCTTCn'nnn_
Cuts at: 0 682 1010 1031 1992
Size: 682 328 21 961
Fragments arranged by size:
961 682 328 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 257 418 613 991 1823 1988 1992
Size: 142 115 161 195 378 832 165 4
Fragments arranged by size:
832 378 195 165 161 142 115 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 362 488 1992
Size: 362 126 1504
Fragments arranged by size:
1504 362 126
EcoO109I rG'GnC_Cy
Cuts at: 0 13 14 367 822 1992
Size: 13 1 353 455 1170
Fragments arranged by size:
1170 455 353 13 1
EcoRV GAT'ATC
Cuts at: 0 1782 1992
Size: 1782 210
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1060 1992
Size: 1060 932
HaeII r_GCGC'y
Cuts at: 0 64 76 1992
Size: 64 12 1916
Fragments arranged by size:
1916 64 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 59 1992
Size: 27 32 1933
Fragments arranged by size:
1933 32 27
HindIII A'AGCT_T
Cuts at: 0 1366 1992
Size: 1366 626
MslI CAynn'nnrTG
Cuts at: 0 1238 1459 1992
Size: 1238 221 533
Fragments arranged by size:
1238 533 221
MspA1I CmG'CkG
Cuts at: 0 1966 1992
Size: 1966 26
NcoI C'CATG_G
Cuts at: 0 1101 1992
Size: 1101 891
NsiI A_TGCA'T
Cuts at: 0 1512 1992
Size: 1512 480
NspI r_CATG'y
Cuts at: 0 645 1068 1510 1992
Size: 645 423 442 482
Fragments arranged by size:
645 482 442 423
PciI A'CATG_T
Cuts at: 0 641 1064 1992
Size: 641 423 928
Fragments arranged by size:
928 641 423
PflMI CCAn_nnn'nTGG
Cuts at: 0 912 1992
Size: 912 1080
PpuMI rG'GwC_Cy
Cuts at: 0 367 822 1992
Size: 367 455 1170
Fragments arranged by size:
1170 455 367
PshAI GACnn'nnGTC
Cuts at: 0 962 1992
Size: 962 1030
PsiI TTA'TAA
Cuts at: 0 841 1992
Size: 841 1151
PspOMI G'GGCC_C
Cuts at: 0 13 1992
Size: 13 1979
PstI C_TGCA'G
Cuts at: 0 1086 1992
Size: 1086 906
PvuII CAG'CTG
Cuts at: 0 1966 1992
Size: 1966 26
SapI GCTCTTCn'nnn_
Cuts at: 0 1010 1992
Size: 1010 982
SfcI C'TryA_G
Cuts at: 0 397 749 1082 1992
Size: 397 352 333 910
Fragments arranged by size:
910 397 352 333
SmlI C'TyrA_G
Cuts at: 0 768 1053 1992
Size: 768 285 939
Fragments arranged by size:
939 768 285
SspI AAT'ATT
Cuts at: 0 926 1992
Size: 926 1066
StyI C'CwwG_G
Cuts at: 0 1101 1992
Size: 1101 891
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 839 1842 1992
Size: 839 1003 150
Fragments arranged by size:
1003 839 150
TatI w'GTAC_w
Cuts at: 0 761 870 1727 1900 1992
Size: 761 109 857 173 92
Fragments arranged by size:
857 761 173 109 92
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlfI AlwNI ApaI
ApoI AseI BanI BanII BbsI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI
BsaHI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI BsrGI
BstAPI BstYI BstZ17I BtgI BtsI DrdI EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HindIII MslI MspA1I NcoI NsiI NspI PciI PflMI
PpuMI PshAI PsiI PspOMI PstI PvuII SapI SfcI
SmlI SspI StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AloI
ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI
BbvCI BclI BglI BmtI BplI BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI
Bsu36I BtgZI ClaI CspCI DraI DraIII EciI EcoICRI
EcoRI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI