Restriction map for
pF1KB7064
(Linear) (Six-base) MAPSORT of: KIBB7064.seq Check: 2220 from: 1 to: 2022
KIBB7064.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 269 335 527 740 1036 1419 2022
Size: 257 12 66 192 213 296 383 603
Fragments arranged by size:
603 383 296 257 213 192 66 12
AhdI GACnn_n'nnGTC
Cuts at: 0 771 2022
Size: 771 1251
AleI CACnn'nnGTG
Cuts at: 0 658 2022
Size: 658 1364
AlwNI CAG_nnn'CTG
Cuts at: 0 776 1442 1844 1993 2022
Size: 776 666 402 149 29
Fragments arranged by size:
776 666 402 149 29
ApaI G_GGCC'C
Cuts at: 0 1431 1741 2022
Size: 1431 310 281
Fragments arranged by size:
1431 310 281
AvaI C'yCGr_G
Cuts at: 0 1757 1832 2022
Size: 1757 75 190
Fragments arranged by size:
1757 190 75
AvrII C'CTAG_G
Cuts at: 0 1573 2022
Size: 1573 449
BamHI G'GATC_C
Cuts at: 0 640 2022
Size: 640 1382
BanI G'GyrC_C
Cuts at: 0 908 2022
Size: 908 1114
BanII G_rGCy'C
Cuts at: 0 131 1431 1511 1569 1741 1813 2022
Size: 131 1300 80 58 172 72 209
Fragments arranged by size:
1300 209 172 131 80 72 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 257 739 1143 2022
Size: 257 482 404 879
Fragments arranged by size:
879 482 404 257
BbvCI CC'TCA_GC
Cuts at: 0 85 870 1118 1487 2022
Size: 85 785 248 369 535
Fragments arranged by size:
785 535 369 248 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1738 1755 1772 1789 2022
Size: 1738 17 17 17 233
Fragments arranged by size:
1738 233 17 17 17
BglI GCCn_nnn'nGGC
Cuts at: 0 110 2022
Size: 110 1912
BlpI GC'TnA_GC
Cuts at: 0 1505 1807 2022
Size: 1505 302 215
Fragments arranged by size:
1505 302 215
Bme1580I G_kGCm'C
Cuts at: 0 1431 1741 1851 2022
Size: 1431 310 110 171
Fragments arranged by size:
1431 310 171 110
BmrI ACTGGGnnnn_n'
Cuts at: 0 64 295 845 911 1503 1921 2022
Size: 64 231 550 66 592 418 101
Fragments arranged by size:
592 550 418 231 101 66 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 2022
Size: 1143 879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 199 287 620 1562 2022
Size: 117 82 88 333 942 460
Fragments arranged by size:
942 460 333 117 88 82
Bpu10I CC'TnA_GC
Cuts at: 0 85 870 1118 1487 2022
Size: 85 785 248 369 535
Fragments arranged by size:
785 535 369 248 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1588 1965 2022
Size: 880 708 377 57
Fragments arranged by size:
880 708 377 57
BsaHI Gr'CG_yC
Cuts at: 0 231 799 2022
Size: 231 568 1223
Fragments arranged by size:
1223 568 231
BsaWI w'CCGG_w
Cuts at: 0 1632 2022
Size: 1632 390
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 188 256 493 526 586 589 820
Size: 73 115 68 237 33 60 3 231
Cuts at: 820 943 1207 1210 1336 1339 1559 1816 1899
Size: 123 264 3 126 3 220 257 83
Cuts at: 1899 2022
Size: 123
Fragments arranged by size:
264 257 237 231 220 126 123 123
115 83 73 68 60 33 3 3
3
BseYI C'CCAG_C
Cuts at: 0 430 649 876 890 1442 2022
Size: 430 219 227 14 552 580
Fragments arranged by size:
580 552 430 227 219 14
BsiHKAI G_wGCw'C
Cuts at: 0 420 2022
Size: 420 1602
Bsp1286I G_dGCh'C
Cuts at: 0 131 420 1431 1511 1569 1741 1813 1851
Size: 131 289 1011 80 58 172 72 38
Cuts at: 1851 2022
Size: 171
Fragments arranged by size:
1011 289 172 171 131 80 72 58
38
BspEI T'CCGG_A
Cuts at: 0 1632 2022
Size: 1632 390
BspHI T'CATG_A
Cuts at: 0 1626 2022
Size: 1626 396
BsrBI CCG'CTC
Cuts at: 0 1973 2022
Size: 1973 49
BsrFI r'CCGG_y
Cuts at: 0 1716 2022
Size: 1716 306
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1993 2022
Size: 1993 29
BstYI r'GATC_y
Cuts at: 0 640 2022
Size: 640 1382
Bsu36I CC'TnA_GG
Cuts at: 0 106 1855 2022
Size: 106 1749 167
Fragments arranged by size:
1749 167 106
BtgI C'CryG_G
Cuts at: 0 34 566 692 2022
Size: 34 532 126 1330
Fragments arranged by size:
1330 532 126 34
BtsI GCAGTG_nn'
Cuts at: 0 448 2022
Size: 448 1574
DraIII CAC_nnn'GTG
Cuts at: 0 1006 2022
Size: 1006 1016
DrdI GACnn_nn'nnGTC
Cuts at: 0 741 2022
Size: 741 1281
EaeI y'GGCC_r
Cuts at: 0 408 1033 1303 1526 1958 2022
Size: 408 625 270 223 432 64
Fragments arranged by size:
625 432 408 270 223 64
EarI CTCTTCn'nnn_
Cuts at: 0 233 488 503 674 920 1049 1100 1134
Size: 233 255 15 171 246 129 51 34
Cuts at: 1134 1469 2022
Size: 335 553
Fragments arranged by size:
553 335 255 246 233 171 129 51
34 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 199 257 269 287 335 527 620
Size: 117 82 58 12 18 48 192 93
Cuts at: 620 740 1036 1419 1562 2022
Size: 120 296 383 143 460
Fragments arranged by size:
460 383 296 192 143 120 117 93
82 58 48 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1142 2022
Size: 1142 880
EcoO109I rG'GnC_Cy
Cuts at: 0 111 118 262 773 887 1427 1737 1738
Size: 111 7 144 511 114 540 310 1
Cuts at: 1738 2022
Size: 284
Fragments arranged by size:
540 511 310 284 144 114 111 7
1
EcoRV GAT'ATC
Cuts at: 0 1450 2022
Size: 1450 572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1686 2022
Size: 1686 336
FseI GG_CCGG'CC
Cuts at: 0 1720 2022
Size: 1720 302
HaeII r_GCGC'y
Cuts at: 0 1611 2022
Size: 1611 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 1072 1078 1104 1110 1207 1239
Size: 757 32 283 6 26 6 97 32
Cuts at: 1239 1514 1546 2022
Size: 275 32 476
Fragments arranged by size:
757 476 283 275 97 32 32 32
26 6 6
HincII GTy'rAC
Cuts at: 0 522 2022
Size: 522 1500
HpaI GTT'AAC
Cuts at: 0 522 2022
Size: 522 1500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1611 2022
Size: 788 823 411
Fragments arranged by size:
823 788 411
MscI TGG'CCA
Cuts at: 0 410 1035 1528 1960 2022
Size: 410 625 493 432 62
Fragments arranged by size:
625 493 432 410 62
MslI CAynn'nnrTG
Cuts at: 0 658 2022
Size: 658 1364
MspA1I CmG'CkG
Cuts at: 0 958 1990 2022
Size: 958 1032 32
Fragments arranged by size:
1032 958 32
NaeI GCC'GGC
Cuts at: 0 1718 2022
Size: 1718 304
NgoMIV G'CCGG_C
Cuts at: 0 1716 2022
Size: 1716 306
NspI r_CATG'y
Cuts at: 0 351 2022
Size: 351 1671
PasI CC'CwG_GG
Cuts at: 0 472 670 856 1562 1742 2022
Size: 472 198 186 706 180 280
Fragments arranged by size:
706 472 280 198 186 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 656 776 1698 1844 1956 2022
Size: 656 120 922 146 112 66
Fragments arranged by size:
922 656 146 120 112 66
PfoI T'CCnGG_A
Cuts at: 0 636 2022
Size: 636 1386
PpuMI rG'GwC_Cy
Cuts at: 0 118 773 2022
Size: 118 655 1249
Fragments arranged by size:
1249 655 118
PspOMI G'GGCC_C
Cuts at: 0 1427 1737 2022
Size: 1427 310 285
Fragments arranged by size:
1427 310 285
PstI C_TGCA'G
Cuts at: 0 1989 2022
Size: 1989 33
PvuII CAG'CTG
Cuts at: 0 958 2022
Size: 958 1064
SfcI C'TryA_G
Cuts at: 0 1142 1985 2022
Size: 1142 843 37
Fragments arranged by size:
1142 843 37
SmaI CCC'GGG
Cuts at: 0 1834 2022
Size: 1834 188
SmlI C'TyrA_G
Cuts at: 0 895 1603 1944 2022
Size: 895 708 341 78
Fragments arranged by size:
895 708 341 78
SrfI GCCC'GGGC
Cuts at: 0 1834 2022
Size: 1834 188
StuI AGG'CCT
Cuts at: 0 1021 1291 2022
Size: 1021 270 731
Fragments arranged by size:
1021 731 270
StyI C'CwwG_G
Cuts at: 0 220 1379 1573 2022
Size: 220 1159 194 449
Fragments arranged by size:
1159 449 220 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 648 1014 1284 1804 1906 2022
Size: 648 366 270 520 102 116
Fragments arranged by size:
648 520 366 270 116 102
XbaI T'CTAG_A
Cuts at: 0 123 2022
Size: 123 1899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1559 2022
Size: 1559 463
XmaI C'CCGG_G
Cuts at: 0 1832 2022
Size: 1832 190
Enzymes that do cut:
AcuI AhdI AleI AlwNI ApaI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BseRI
BseYI BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrFI BstAPI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRV FalI FseI HaeII Hin4I
HincII HpaI MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SfcI SmaI SmlI SrfI StuI StyI TaqII XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BbeI BciVI BclI BfrBI BglII BmgBI BmtI
BsaI BsaAI BsaBI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EciI
EcoICRI EcoRI FspI FspAI HindIII KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI TatI TstI Tth111I XhoI
XmnI ZraI