(Linear) (Six-base) MAPSORT of: KIBB7085.seq  Check: 8316  from: 1  to: 387



KIBB7085.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AccI GT'mk_AC

Cuts at:      0     239     387 
   Size:        239     148
 
AflIII A'CryG_T

Cuts at:      0      77     387 
   Size:         77     310
 
AleI CACnn'nnGTG

Cuts at:      0      22     387 
   Size:         22     365
 
AvaI C'yCGr_G

Cuts at:      0     290     387 
   Size:        290      97
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     235     269     387 
   Size:        235      34     118

  Fragments arranged by size: 

                 235     118      34
 
BlpI GC'TnA_GC

Cuts at:      0     187     387 
   Size:        187     200
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     160     387 
   Size:        160     227
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     232     319     387 
   Size:        145      87      87      68

  Fragments arranged by size: 

                 145      87      87      68
 
BsaHI Gr'CG_yC

Cuts at:      0      28     387 
   Size:         28     359
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     184     214     387 
   Size:        184      30     173

  Fragments arranged by size: 

                 184     173      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     218     387 
   Size:        218     169
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     364     387 
   Size:        364      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     387 
   Size:        218     169
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      86     387 
   Size:         86     301
 
BsrBI CCG'CTC

Cuts at:      0     200     387 
   Size:        200     187
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     387 
   Size:        210     177
 
EaeI y'GGCC_r

Cuts at:      0     338     387 
   Size:        338      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     232     319     387 
   Size:        145      87      87      68

  Fragments arranged by size: 

                 145      87      87      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0      98     129     294     387 
   Size:         98      31     165      93

  Fragments arranged by size: 

                 165      98      93      31
 
MscI TGG'CCA

Cuts at:      0     340     387 
   Size:        340      47
 
MslI CAynn'nnrTG

Cuts at:      0      22     387 
   Size:         22     365
 
MspA1I CmG'CkG

Cuts at:      0      22     110     387 
   Size:         22      88     277

  Fragments arranged by size: 

                 277      88      22
 
NspI r_CATG'y

Cuts at:      0      81     387 
   Size:         81     306
 
PasI CC'CwG_GG

Cuts at:      0     174     387 
   Size:        174     213
 
PciI A'CATG_T

Cuts at:      0      77     387 
   Size:         77     310
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      47     387 
   Size:         47     340
 
PfoI T'CCnGG_A

Cuts at:      0     296     387 
   Size:        296      91
 
PpuMI rG'GwC_Cy

Cuts at:      0      98     294     387 
   Size:         98     196      93

  Fragments arranged by size: 

                 196      98      93
 
SfcI C'TryA_G

Cuts at:      0     306     387 
   Size:        306      81
 
SmaI CCC'GGG

Cuts at:      0     292     387 
   Size:        292      95
 
StuI AGG'CCT

Cuts at:      0     277     387 
   Size:        277     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     348     380     387 
   Size:        348      32       7

  Fragments arranged by size: 

                 348      32       7
 
XmaI C'CCGG_G

Cuts at:      0     290     387 
   Size:        290      97
 

 Enzymes that do cut:

     AccI   AflIII     AleI     AvaI     BcgI     BlpI     BmrI     BpmI
    BsaHI    BsaXI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BstXI
     EaeI  Eco57MI EcoO109I     MscI     MslI   MspA1I     NspI     PasI
     PciI    PflMI     PfoI    PpuMI     SfcI     SmaI     StuI     TstI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI