(Linear) (Six-base) MAPSORT of: KIBB7087.seq Check: 2758 from: 1 to: 402
KIBB7087.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
Acc65I G'GTAC_C
Cuts at: 0 142 402
Size: 142 260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 402
Size: 26 376
AleI CACnn'nnGTG
Cuts at: 0 217 402
Size: 217 185
BanI G'GyrC_C
Cuts at: 0 142 402
Size: 142 260
BanII G_rGCy'C
Cuts at: 0 14 36 73 152 402
Size: 14 22 37 79 250
Fragments arranged by size:
250 79 37 22 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 183 402
Size: 183 219
BclI T'GATC_A
Cuts at: 0 89 402
Size: 89 313
BglI GCCn_nnn'nGGC
Cuts at: 0 40 402
Size: 40 362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 402
Size: 288 114
BsiHKAI G_wGCw'C
Cuts at: 0 152 402
Size: 152 250
BsmBI CGTCTCn'nnnn_
Cuts at: 0 345 402
Size: 345 57
Bsp1286I G_dGCh'C
Cuts at: 0 14 36 73 152 402
Size: 14 22 37 79 250
Fragments arranged by size:
250 79 37 22 14
BsrFI r'CCGG_y
Cuts at: 0 199 402
Size: 199 203
BssSI C'ACGA_G
Cuts at: 0 373 402
Size: 373 29
BstEII G'GTnAC_C
Cuts at: 0 210 402
Size: 210 192
BstYI r'GATC_y
Cuts at: 0 23 402
Size: 23 379
BtgI C'CryG_G
Cuts at: 0 215 240 402
Size: 215 25 162
Fragments arranged by size:
215 162 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 180 402
Size: 180 222
DrdI GACnn_nn'nnGTC
Cuts at: 0 359 402
Size: 359 43
EarI CTCTTCn'nnn_
Cuts at: 0 2 61 402
Size: 2 59 341
Fragments arranged by size:
341 59 2
EcoICRI GAG'CTC
Cuts at: 0 150 402
Size: 150 252
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 288 402
Size: 26 262 114
Fragments arranged by size:
262 114 26
HindIII A'AGCT_T
Cuts at: 0 390 402
Size: 390 12
KpnI G_GTAC'C
Cuts at: 0 146 402
Size: 146 256
MslI CAynn'nnrTG
Cuts at: 0 217 402
Size: 217 185
MspA1I CmG'CkG
Cuts at: 0 164 217 402
Size: 164 53 185
Fragments arranged by size:
185 164 53
NaeI GCC'GGC
Cuts at: 0 201 402
Size: 201 201
NcoI C'CATG_G
Cuts at: 0 240 402
Size: 240 162
NgoMIV G'CCGG_C
Cuts at: 0 199 402
Size: 199 203
SacI G_AGCT'C
Cuts at: 0 152 402
Size: 152 250
SacII CC_GC'GG
Cuts at: 0 218 402
Size: 218 184
SapI GCTCTTCn'nnn_
Cuts at: 0 2 61 402
Size: 2 59 341
Fragments arranged by size:
341 59 2
SfcI C'TryA_G
Cuts at: 0 134 402
Size: 134 268
StyI C'CwwG_G
Cuts at: 0 240 402
Size: 240 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 264 402
Size: 102 162 138
Fragments arranged by size:
162 138 102
Enzymes that do cut:
Acc65I AcuI AleI BanI BanII BbsI BclI BglI
BpmI BsiHKAI BsmBI Bsp1286I BsrFI BssSI BstEII BstYI
BtgI BtgZI DrdI EarI EcoICRI Eco57MI HindIII KpnI
MslI MspA1I NaeI NcoI NgoMIV SacI SacII SapI
SfcI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HpaI
KasI MfeI MluI MmeI MscI NarI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI