Restriction map for pF1KB7087
 (Linear) (Six-base) MAPSORT of: KIBB7087.seq  Check: 2758  from: 1  to: 402



KIBB7087.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

Acc65I G'GTAC_C

Cuts at:      0     142     402 
   Size:        142     260
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     402 
   Size:         26     376
 
AleI CACnn'nnGTG

Cuts at:      0     217     402 
   Size:        217     185
 
BanI G'GyrC_C

Cuts at:      0     142     402 
   Size:        142     260
 
BanII G_rGCy'C

Cuts at:      0      14      36      73     152     402 
   Size:         14      22      37      79     250

  Fragments arranged by size: 

                 250      79      37      22      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     183     402 
   Size:        183     219
 
BclI T'GATC_A

Cuts at:      0      89     402 
   Size:         89     313
 
BglI GCCn_nnn'nGGC

Cuts at:      0      40     402 
   Size:         40     362
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     402 
   Size:        288     114
 
BsiHKAI G_wGCw'C

Cuts at:      0     152     402 
   Size:        152     250
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     345     402 
   Size:        345      57
 
Bsp1286I G_dGCh'C

Cuts at:      0      14      36      73     152     402 
   Size:         14      22      37      79     250

  Fragments arranged by size: 

                 250      79      37      22      14
 
BsrFI r'CCGG_y

Cuts at:      0     199     402 
   Size:        199     203
 
BssSI C'ACGA_G

Cuts at:      0     373     402 
   Size:        373      29
 
BstEII G'GTnAC_C

Cuts at:      0     210     402 
   Size:        210     192
 
BstYI r'GATC_y

Cuts at:      0      23     402 
   Size:         23     379
 
BtgI C'CryG_G

Cuts at:      0     215     240     402 
   Size:        215      25     162

  Fragments arranged by size: 

                 215     162      25
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     180     402 
   Size:        180     222
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     359     402 
   Size:        359      43
 
EarI CTCTTCn'nnn_

Cuts at:      0       2      61     402 
   Size:          2      59     341

  Fragments arranged by size: 

                 341      59       2
 
EcoICRI GAG'CTC

Cuts at:      0     150     402 
   Size:        150     252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     288     402 
   Size:         26     262     114

  Fragments arranged by size: 

                 262     114      26
 
HindIII A'AGCT_T

Cuts at:      0     390     402 
   Size:        390      12
 
KpnI G_GTAC'C

Cuts at:      0     146     402 
   Size:        146     256
 
MslI CAynn'nnrTG

Cuts at:      0     217     402 
   Size:        217     185
 
MspA1I CmG'CkG

Cuts at:      0     164     217     402 
   Size:        164      53     185

  Fragments arranged by size: 

                 185     164      53
 
NaeI GCC'GGC

Cuts at:      0     201     402 
   Size:        201     201
 
NcoI C'CATG_G

Cuts at:      0     240     402 
   Size:        240     162
 
NgoMIV G'CCGG_C

Cuts at:      0     199     402 
   Size:        199     203
 
SacI G_AGCT'C

Cuts at:      0     152     402 
   Size:        152     250
 
SacII CC_GC'GG

Cuts at:      0     218     402 
   Size:        218     184
 
SapI GCTCTTCn'nnn_

Cuts at:      0       2      61     402 
   Size:          2      59     341

  Fragments arranged by size: 

                 341      59       2
 
SfcI C'TryA_G

Cuts at:      0     134     402 
   Size:        134     268
 
StyI C'CwwG_G

Cuts at:      0     240     402 
   Size:        240     162
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     102     264     402 
   Size:        102     162     138

  Fragments arranged by size: 

                 162     138     102
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     BanI    BanII     BbsI     BclI     BglI
     BpmI  BsiHKAI    BsmBI Bsp1286I    BsrFI    BssSI   BstEII    BstYI
     BtgI    BtgZI     DrdI     EarI  EcoICRI  Eco57MI  HindIII     KpnI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     SacI    SacII     SapI
     SfcI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     MfeI     MluI     MmeI     MscI     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com