(Linear) (Six-base) MAPSORT of: KIBB7087.seq Check: 2758 from: 1 to: 402 KIBB7087.orf With 182 enzymes: SgfI * March 3, 2008 10:58 .. Acc65I G'GTAC_C Cuts at: 0 142 402 Size: 142 260 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 402 Size: 26 376 AleI CACnn'nnGTG Cuts at: 0 217 402 Size: 217 185 BanI G'GyrC_C Cuts at: 0 142 402 Size: 142 260 BanII G_rGCy'C Cuts at: 0 14 36 73 152 402 Size: 14 22 37 79 250 Fragments arranged by size: 250 79 37 22 14 BbsI GAAGACnn'nnnn_ Cuts at: 0 183 402 Size: 183 219 BclI T'GATC_A Cuts at: 0 89 402 Size: 89 313 BglI GCCn_nnn'nGGC Cuts at: 0 40 402 Size: 40 362 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 288 402 Size: 288 114 BsiHKAI G_wGCw'C Cuts at: 0 152 402 Size: 152 250 BsmBI CGTCTCn'nnnn_ Cuts at: 0 345 402 Size: 345 57 Bsp1286I G_dGCh'C Cuts at: 0 14 36 73 152 402 Size: 14 22 37 79 250 Fragments arranged by size: 250 79 37 22 14 BsrFI r'CCGG_y Cuts at: 0 199 402 Size: 199 203 BssSI C'ACGA_G Cuts at: 0 373 402 Size: 373 29 BstEII G'GTnAC_C Cuts at: 0 210 402 Size: 210 192 BstYI r'GATC_y Cuts at: 0 23 402 Size: 23 379 BtgI C'CryG_G Cuts at: 0 215 240 402 Size: 215 25 162 Fragments arranged by size: 215 162 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 180 402 Size: 180 222 DrdI GACnn_nn'nnGTC Cuts at: 0 359 402 Size: 359 43 EarI CTCTTCn'nnn_ Cuts at: 0 2 61 402 Size: 2 59 341 Fragments arranged by size: 341 59 2 EcoICRI GAG'CTC Cuts at: 0 150 402 Size: 150 252 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 288 402 Size: 26 262 114 Fragments arranged by size: 262 114 26 HindIII A'AGCT_T Cuts at: 0 390 402 Size: 390 12 KpnI G_GTAC'C Cuts at: 0 146 402 Size: 146 256 MslI CAynn'nnrTG Cuts at: 0 217 402 Size: 217 185 MspA1I CmG'CkG Cuts at: 0 164 217 402 Size: 164 53 185 Fragments arranged by size: 185 164 53 NaeI GCC'GGC Cuts at: 0 201 402 Size: 201 201 NcoI C'CATG_G Cuts at: 0 240 402 Size: 240 162 NgoMIV G'CCGG_C Cuts at: 0 199 402 Size: 199 203 SacI G_AGCT'C Cuts at: 0 152 402 Size: 152 250 SacII CC_GC'GG Cuts at: 0 218 402 Size: 218 184 SapI GCTCTTCn'nnn_ Cuts at: 0 2 61 402 Size: 2 59 341 Fragments arranged by size: 341 59 2 SfcI C'TryA_G Cuts at: 0 134 402 Size: 134 268 StyI C'CwwG_G Cuts at: 0 240 402 Size: 240 162 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 102 264 402 Size: 102 162 138 Fragments arranged by size: 162 138 102 Enzymes that do cut: Acc65I AcuI AleI BanI BanII BbsI BclI BglI BpmI BsiHKAI BsmBI Bsp1286I BsrFI BssSI BstEII BstYI BtgI BtgZI DrdI EarI EcoICRI Eco57MI HindIII KpnI MslI MspA1I NaeI NcoI NgoMIV SacI SacII SapI SfcI StyI XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EaeI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HpaI KasI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI