Restriction map for
pF1KB7090
(Linear) (Six-base) MAPSORT of: KIBB7090.seq Check: 9794 from: 1 to: 510
KIBB7090.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 279 510
Size: 279 231
ApaLI G'TGCA_C
Cuts at: 0 400 510
Size: 400 110
ApoI r'AATT_y
Cuts at: 0 504 510
Size: 504 6
BanI G'GyrC_C
Cuts at: 0 37 291 327 510
Size: 37 254 36 183
Fragments arranged by size:
254 183 37 36
BanII G_rGCy'C
Cuts at: 0 379 510
Size: 379 131
BbeI G_GCGC'C
Cuts at: 0 331 510
Size: 331 179
Bme1580I G_kGCm'C
Cuts at: 0 296 404 510
Size: 296 108 106
Fragments arranged by size:
296 108 106
BmgBI CAC'GTC
Cuts at: 0 399 510
Size: 399 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 465 510
Size: 405 60 45
Fragments arranged by size:
405 60 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 82 460 510
Size: 33 49 378 50
Fragments arranged by size:
378 50 49 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 510
Size: 439 71
BsaBI GATnn'nnATC
Cuts at: 0 154 510
Size: 154 356
BsaHI Gr'CG_yC
Cuts at: 0 328 457 510
Size: 328 129 53
Fragments arranged by size:
328 129 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 510
Size: 181 30 299
Fragments arranged by size:
299 181 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 241 378 433 448 457 510
Size: 241 137 55 15 9 53
Fragments arranged by size:
241 137 55 53 15 9
BseYI C'CCAG_C
Cuts at: 0 162 510
Size: 162 348
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 327 510
Size: 307 20 183
Fragments arranged by size:
307 183 20
BsiEI CG_ry'CG
Cuts at: 0 77 510
Size: 77 433
BsiHKAI G_wGCw'C
Cuts at: 0 379 404 510
Size: 379 25 106
Fragments arranged by size:
379 106 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 448 510
Size: 448 62
Bsp1286I G_dGCh'C
Cuts at: 0 296 379 404 510
Size: 296 83 25 106
Fragments arranged by size:
296 106 83 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 279 510
Size: 279 231
BsrBI CCG'CTC
Cuts at: 0 231 510
Size: 231 279
BsrDI GCAATG_nn'
Cuts at: 0 99 510
Size: 99 411
BsrFI r'CCGG_y
Cuts at: 0 76 510
Size: 76 434
BsrGI T'GTAC_A
Cuts at: 0 215 510
Size: 215 295
Bsu36I CC'TnA_GG
Cuts at: 0 277 510
Size: 277 233
BtgI C'CryG_G
Cuts at: 0 263 356 510
Size: 263 93 154
Fragments arranged by size:
263 154 93
EaeI y'GGCC_r
Cuts at: 0 74 510
Size: 74 436
EagI C'GGCC_G
Cuts at: 0 74 510
Size: 74 436
EcoICRI GAG'CTC
Cuts at: 0 377 510
Size: 377 133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 82 460 510
Size: 33 49 378 50
Fragments arranged by size:
378 50 49 33
EcoO109I rG'GnC_Cy
Cuts at: 0 482 510
Size: 482 28
EcoRI G'AATT_C
Cuts at: 0 504 510
Size: 504 6
HaeII r_GCGC'y
Cuts at: 0 331 510
Size: 331 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 510
Size: 181 32 297
Fragments arranged by size:
297 181 32
KasI G'GCGC_C
Cuts at: 0 327 510
Size: 327 183
MspA1I CmG'CkG
Cuts at: 0 89 258 510
Size: 89 169 252
Fragments arranged by size:
252 169 89
NarI GG'CG_CC
Cuts at: 0 328 510
Size: 328 182
NcoI C'CATG_G
Cuts at: 0 356 510
Size: 356 154
NspI r_CATG'y
Cuts at: 0 312 510
Size: 312 198
PpuMI rG'GwC_Cy
Cuts at: 0 482 510
Size: 482 28
PstI C_TGCA'G
Cuts at: 0 317 510
Size: 317 193
SacI G_AGCT'C
Cuts at: 0 379 510
Size: 379 131
ScaI AGT'ACT
Cuts at: 0 106 510
Size: 106 404
SfcI C'TryA_G
Cuts at: 0 313 510
Size: 313 197
SfoI GGC'GCC
Cuts at: 0 329 510
Size: 329 181
StyI C'CwwG_G
Cuts at: 0 356 510
Size: 356 154
TatI w'GTAC_w
Cuts at: 0 104 215 510
Size: 104 111 295
Fragments arranged by size:
295 111 104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 510
Size: 57 453
Enzymes that do cut:
AarI ApaLI ApoI BanI BanII BbeI Bme1580I BmgBI
BplI BpmI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI Bsu36I BtgI EaeI EagI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I KasI MspA1I NarI NcoI
NspI PpuMI PstI SacI ScaI SfcI SfoI StyI
TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmrI
BmtI Bpu10I BpuEI BsaAI BsaWI BsiWI BsmI BspEI
BspHI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoNI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
XmnI ZraI