Restriction map for
pF1KB7096
(Linear) (Six-base) MAPSORT of: KIBB7096.seq Check: 9498 from: 1 to: 579
KIBB7096.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AccI GT'mk_AC
Cuts at: 0 200 579
Size: 200 379
AvaI C'yCGr_G
Cuts at: 0 477 579
Size: 477 102
BanI G'GyrC_C
Cuts at: 0 54 303 425 467 579
Size: 54 249 122 42 112
Fragments arranged by size:
249 122 112 54 42
BanII G_rGCy'C
Cuts at: 0 65 579
Size: 65 514
BbeI G_GCGC'C
Cuts at: 0 58 471 579
Size: 58 413 108
Fragments arranged by size:
413 108 58
BciVI GTATCCnnnnn_n'
Cuts at: 0 131 579
Size: 131 448
BlpI GC'TnA_GC
Cuts at: 0 569 579
Size: 569 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 392 579
Size: 392 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 337 387 442 517 579
Size: 245 92 50 55 75 62
Fragments arranged by size:
245 92 75 62 55 50
BsaHI Gr'CG_yC
Cuts at: 0 55 468 579
Size: 55 413 111
Fragments arranged by size:
413 111 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 335 365 579
Size: 335 30 214
Fragments arranged by size:
335 214 30
BseYI C'CCAG_C
Cuts at: 0 65 579
Size: 65 514
BsiHKAI G_wGCw'C
Cuts at: 0 238 579
Size: 238 341
Bsp1286I G_dGCh'C
Cuts at: 0 65 238 579
Size: 65 173 341
Fragments arranged by size:
341 173 65
BstYI r'GATC_y
Cuts at: 0 557 579
Size: 557 22
BtgI C'CryG_G
Cuts at: 0 103 463 579
Size: 103 360 116
Fragments arranged by size:
360 116 103
DrdI GACnn_nn'nnGTC
Cuts at: 0 409 579
Size: 409 170
EaeI y'GGCC_r
Cuts at: 0 277 579
Size: 277 302
EarI CTCTTCn'nnn_
Cuts at: 0 116 308 579
Size: 116 192 271
Fragments arranged by size:
271 192 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 337 387 442 517 579
Size: 245 92 50 55 75 62
Fragments arranged by size:
245 92 75 62 55 50
HaeII r_GCGC'y
Cuts at: 0 58 471 579
Size: 58 413 108
Fragments arranged by size:
413 108 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 579
Size: 335 32 212
Fragments arranged by size:
335 212 32
KasI G'GCGC_C
Cuts at: 0 54 467 579
Size: 54 413 112
Fragments arranged by size:
413 112 54
MspA1I CmG'CkG
Cuts at: 0 152 422 579
Size: 152 270 157
Fragments arranged by size:
270 157 152
NarI GG'CG_CC
Cuts at: 0 55 468 579
Size: 55 413 111
Fragments arranged by size:
413 111 55
PasI CC'CwG_GG
Cuts at: 0 386 579
Size: 386 193
PfoI T'CCnGG_A
Cuts at: 0 222 579
Size: 222 357
PvuII CAG'CTG
Cuts at: 0 152 579
Size: 152 427
ScaI AGT'ACT
Cuts at: 0 128 139 579
Size: 128 11 440
Fragments arranged by size:
440 128 11
SfoI GGC'GCC
Cuts at: 0 56 469 579
Size: 56 413 110
Fragments arranged by size:
413 110 56
SmaI CCC'GGG
Cuts at: 0 479 579
Size: 479 100
StyI C'CwwG_G
Cuts at: 0 239 579
Size: 239 340
TatI w'GTAC_w
Cuts at: 0 126 137 250 534 579
Size: 126 11 113 284 45
Fragments arranged by size:
284 126 113 45 11
XmaI C'CCGG_G
Cuts at: 0 477 579
Size: 477 102
Enzymes that do cut:
AccI AvaI BanI BanII BbeI BciVI BlpI BplI
BpmI BsaHI BsaXI BseYI BsiHKAI Bsp1286I BstYI BtgI
DrdI EaeI EarI Eco57MI HaeII Hin4I KasI MspA1I
NarI PasI PfoI PvuII ScaI SfoI SmaI StyI
TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BclI BfrBI BglI BglII Bme1580I BmgBI
BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI ZraI