Restriction map for
pF1KB7098
(Linear) (Six-base) MAPSORT of: KIBB7098.seq Check: 7347 from: 1 to: 594
KIBB7098.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 169 594
Size: 169 425
AatII G_ACGT'C
Cuts at: 0 551 594
Size: 551 43
AccI GT'mk_AC
Cuts at: 0 505 594
Size: 505 89
AflIII A'CryG_T
Cuts at: 0 392 594
Size: 392 202
AleI CACnn'nnGTG
Cuts at: 0 322 594
Size: 322 272
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 350 594
Size: 350 244
ApaLI G'TGCA_C
Cuts at: 0 325 594
Size: 325 269
ApoI r'AATT_y
Cuts at: 0 382 594
Size: 382 212
BanI G'GyrC_C
Cuts at: 0 134 594
Size: 134 460
BanII G_rGCy'C
Cuts at: 0 7 594
Size: 7 587
BlpI GC'TnA_GC
Cuts at: 0 351 594
Size: 351 243
Bme1580I G_kGCm'C
Cuts at: 0 329 594
Size: 329 265
BmrI ACTGGGnnnn_n'
Cuts at: 0 426 594
Size: 426 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 424 483 594
Size: 127 297 59 111
Fragments arranged by size:
297 127 111 59
BsaHI Gr'CG_yC
Cuts at: 0 548 594
Size: 548 46
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 84 130 594
Size: 84 46 464
Fragments arranged by size:
464 84 46
BsiHKAI G_wGCw'C
Cuts at: 0 7 329 594
Size: 7 322 265
Fragments arranged by size:
322 265 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 329 594
Size: 7 322 265
Fragments arranged by size:
322 265 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 169 594
Size: 169 425
BsrBI CCG'CTC
Cuts at: 0 350 594
Size: 350 244
BsrDI GCAATG_nn'
Cuts at: 0 77 594
Size: 77 517
BssSI C'ACGA_G
Cuts at: 0 149 594
Size: 149 445
BstAPI GCAn_nnn'nTGC
Cuts at: 0 161 594
Size: 161 433
BstXI CCAn_nnnn'nTGG
Cuts at: 0 151 496 594
Size: 151 345 98
Fragments arranged by size:
345 151 98
BstYI r'GATC_y
Cuts at: 0 185 287 594
Size: 185 102 307
Fragments arranged by size:
307 185 102
BtgI C'CryG_G
Cuts at: 0 275 594
Size: 275 319
BtsI GCAGTG_nn'
Cuts at: 0 123 594
Size: 123 471
DraIII CAC_nnn'GTG
Cuts at: 0 297 362 393 594
Size: 297 65 31 201
Fragments arranged by size:
297 201 65 31
EcoICRI GAG'CTC
Cuts at: 0 5 594
Size: 5 589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 424 483 594
Size: 127 297 59 111
Fragments arranged by size:
297 127 111 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 142 403 594
Size: 142 261 191
Fragments arranged by size:
261 191 142
HaeII r_GCGC'y
Cuts at: 0 489 594
Size: 489 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 425 457 547 579 594
Size: 425 32 90 32 15
Fragments arranged by size:
425 90 32 32 15
HincII GTy'rAC
Cuts at: 0 506 594
Size: 506 88
MslI CAynn'nnrTG
Cuts at: 0 322 594
Size: 322 272
NspI r_CATG'y
Cuts at: 0 243 250 594
Size: 243 7 344
Fragments arranged by size:
344 243 7
PstI C_TGCA'G
Cuts at: 0 339 594
Size: 339 255
SacI G_AGCT'C
Cuts at: 0 7 594
Size: 7 587
SalI G'TCGA_C
Cuts at: 0 504 594
Size: 504 90
SfcI C'TryA_G
Cuts at: 0 335 594
Size: 335 259
SphI G_CATG'C
Cuts at: 0 250 594
Size: 250 344
StyI C'CwwG_G
Cuts at: 0 28 320 594
Size: 28 292 274
Fragments arranged by size:
292 274 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 408 594
Size: 408 186
TatI w'GTAC_w
Cuts at: 0 263 594
Size: 263 331
ZraI GAC'GTC
Cuts at: 0 549 594
Size: 549 45
Enzymes that do cut:
AarI AatII AccI AflIII AleI AlfI ApaLI ApoI
BanI BanII BlpI Bme1580I BmrI BpmI BsaHI BseRI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BssSI BstAPI BstXI
BstYI BtgI BtsI DraIII EcoICRI Eco57MI EcoNI HaeII
Hin4I HincII MslI NspI PstI SacI SalI SfcI
SphI StyI TaqII TatI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AgeI AhdI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI