Restriction map for
pF1KB7101
(Linear) (Six-base) MAPSORT of: KIBB7101.seq Check: 5581 from: 1 to: 639
KIBB7101.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 551 639
Size: 235 316 88
Fragments arranged by size:
316 235 88
AlwNI CAG_nnn'CTG
Cuts at: 0 612 639
Size: 612 27
ApaI G_GGCC'C
Cuts at: 0 444 639
Size: 444 195
AvaI C'yCGr_G
Cuts at: 0 517 639
Size: 517 122
AvrII C'CTAG_G
Cuts at: 0 501 639
Size: 501 138
BamHI G'GATC_C
Cuts at: 0 3 419 639
Size: 3 416 220
Fragments arranged by size:
416 220 3
BanI G'GyrC_C
Cuts at: 0 505 639
Size: 505 134
BanII G_rGCy'C
Cuts at: 0 142 444 639
Size: 142 302 195
Fragments arranged by size:
302 195 142
BbsI GAAGACnn'nnnn_
Cuts at: 0 217 639
Size: 217 422
BglI GCCn_nnn'nGGC
Cuts at: 0 485 639
Size: 485 154
Bme1580I G_kGCm'C
Cuts at: 0 444 639
Size: 444 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 526 639
Size: 262 264 113
Fragments arranged by size:
264 262 113
Bpu10I CC'TnA_GC
Cuts at: 0 136 639
Size: 136 503
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 639
Size: 601 38
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 220 297 300 444 574 639
Size: 220 77 3 144 130 65
Fragments arranged by size:
220 144 130 77 65 3
BseYI C'CCAG_C
Cuts at: 0 154 639
Size: 154 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 639
Size: 493 146
BsmI GAATG_Cn'
Cuts at: 0 265 639
Size: 265 374
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 639
Size: 457 182
Bsp1286I G_dGCh'C
Cuts at: 0 142 444 639
Size: 142 302 195
Fragments arranged by size:
302 195 142
BsrBI CCG'CTC
Cuts at: 0 225 639
Size: 225 414
BstYI r'GATC_y
Cuts at: 0 3 419 639
Size: 3 416 220
Fragments arranged by size:
416 220 3
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 14 639
Size: 14 625
BtsI GCAGTG_nn'
Cuts at: 0 628 639
Size: 628 11
ClaI AT'CG_AT
Cuts at: 0 366 639
Size: 366 273
EarI CTCTTCn'nnn_
Cuts at: 0 215 639
Size: 215 424
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 178 639
Size: 178 461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 262 526 551 639
Size: 235 27 264 25 88
Fragments arranged by size:
264 235 88 27 25
EcoO109I rG'GnC_Cy
Cuts at: 0 440 441 477 639
Size: 440 1 36 162
Fragments arranged by size:
440 162 36 1
HaeII r_GCGC'y
Cuts at: 0 88 570 639
Size: 88 482 69
Fragments arranged by size:
482 88 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 129 161 639
Size: 129 32 478
Fragments arranged by size:
478 129 32
MspA1I CmG'CkG
Cuts at: 0 58 195 639
Size: 58 137 444
Fragments arranged by size:
444 137 58
NspI r_CATG'y
Cuts at: 0 280 639
Size: 280 359
PasI CC'CwG_GG
Cuts at: 0 424 639
Size: 424 215
PshAI GACnn'nnGTC
Cuts at: 0 356 639
Size: 356 283
PspOMI G'GGCC_C
Cuts at: 0 440 639
Size: 440 199
PstI C_TGCA'G
Cuts at: 0 63 639
Size: 63 576
PvuII CAG'CTG
Cuts at: 0 58 639
Size: 58 581
SapI GCTCTTCn'nnn_
Cuts at: 0 215 639
Size: 215 424
SfcI C'TryA_G
Cuts at: 0 59 639
Size: 59 580
SmaI CCC'GGG
Cuts at: 0 519 639
Size: 519 120
SmlI C'TyrA_G
Cuts at: 0 616 639
Size: 616 23
SrfI GCCC'GGGC
Cuts at: 0 519 639
Size: 519 120
StyI C'CwwG_G
Cuts at: 0 501 639
Size: 501 138
XmaI C'CCGG_G
Cuts at: 0 517 639
Size: 517 122
XmnI GAAnn'nnTTC
Cuts at: 0 265 367 639
Size: 265 102 272
Fragments arranged by size:
272 265 102
Enzymes that do cut:
AcuI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbsI BglI Bme1580I BpmI Bpu10I BpuEI BseRI BseYI
BsgI BsmI BsmBI Bsp1286I BsrBI BstYI BtgZI BtsI
ClaI EarI EciI Eco57MI EcoO109I HaeII Hin4I MspA1I
NspI PasI PshAI PspOMI PstI PvuII SapI SfcI
SmaI SmlI SrfI StyI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI CspCI
DraI DraIII DrdI EaeI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI ZraI