Restriction map for
pF1KB7105
(Linear) (Six-base) MAPSORT of: KIBB7105.seq Check: 9531 from: 1 to: 672
KIBB7105.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 381 672
Size: 381 291
AfeI AGC'GCT
Cuts at: 0 271 672
Size: 271 401
AleI CACnn'nnGTG
Cuts at: 0 148 214 337 672
Size: 148 66 123 335
Fragments arranged by size:
335 148 123 66
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 152 184 672
Size: 152 32 488
Fragments arranged by size:
488 152 32
ApaI G_GGCC'C
Cuts at: 0 44 89 321 415 461 672
Size: 44 45 232 94 46 211
Fragments arranged by size:
232 211 94 46 45 44
ApaLI G'TGCA_C
Cuts at: 0 203 672
Size: 203 469
ApoI r'AATT_y
Cuts at: 0 390 672
Size: 390 282
AvaI C'yCGr_G
Cuts at: 0 263 356 514 672
Size: 263 93 158 158
Fragments arranged by size:
263 158 158 93
BamHI G'GATC_C
Cuts at: 0 585 672
Size: 585 87
BanI G'GyrC_C
Cuts at: 0 221 256 672
Size: 221 35 416
Fragments arranged by size:
416 221 35
BanII G_rGCy'C
Cuts at: 0 44 89 251 321 415 461 553 583
Size: 44 45 162 70 94 46 92 30
Cuts at: 583 672
Size: 89
Fragments arranged by size:
162 94 92 89 70 46 45 44
30
BbeI G_GCGC'C
Cuts at: 0 225 672
Size: 225 447
BbvCI CC'TCA_GC
Cuts at: 0 381 672
Size: 381 291
BglI GCCn_nnn'nGGC
Cuts at: 0 27 84 121 672
Size: 27 57 37 551
Fragments arranged by size:
551 57 37 27
BglII A'GATC_T
Cuts at: 0 626 672
Size: 626 46
Bme1580I G_kGCm'C
Cuts at: 0 44 89 207 259 321 346 415 461
Size: 44 45 118 52 62 25 69 46
Cuts at: 461 672
Size: 211
Fragments arranged by size:
211 118 69 62 52 46 45 44
25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 645 672
Size: 39 606 27
Fragments arranged by size:
606 39 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 586 672
Size: 448 138 86
Fragments arranged by size:
448 138 86
Bpu10I CC'TnA_GC
Cuts at: 0 323 381 672
Size: 323 58 291
Fragments arranged by size:
323 291 58
BsaHI Gr'CG_yC
Cuts at: 0 222 672
Size: 222 450
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 245 672
Size: 245 427
BseYI C'CCAG_C
Cuts at: 0 193 400 435 461 503 672
Size: 193 207 35 26 42 169
Fragments arranged by size:
207 193 169 42 35 26
BsiHKAI G_wGCw'C
Cuts at: 0 180 207 448 672
Size: 180 27 241 224
Fragments arranged by size:
241 224 180 27
Bsp1286I G_dGCh'C
Cuts at: 0 44 89 180 207 251 259 321 346
Size: 44 45 91 27 44 8 62 25
Cuts at: 346 415 448 461 553 583 672
Size: 69 33 13 92 30 89
Fragments arranged by size:
92 91 89 69 62 45 44 44
33 30 27 25 13 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 381 672
Size: 381 291
BstEII G'GTnAC_C
Cuts at: 0 612 672
Size: 612 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 339 408 495 672
Size: 105 234 69 87 177
Fragments arranged by size:
234 177 105 87 69
BstYI r'GATC_y
Cuts at: 0 585 626 672
Size: 585 41 46
Fragments arranged by size:
585 46 41
BtgI C'CryG_G
Cuts at: 0 64 251 607 672
Size: 64 187 356 65
Fragments arranged by size:
356 187 65 64
BtsI GCAGTG_nn'
Cuts at: 0 73 672
Size: 73 599
EaeI y'GGCC_r
Cuts at: 0 113 571 672
Size: 113 458 101
Fragments arranged by size:
458 113 101
EarI CTCTTCn'nnn_
Cuts at: 0 136 672
Size: 136 536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 160 172 672
Size: 160 12 500
Fragments arranged by size:
500 160 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 586 672
Size: 448 138 86
Fragments arranged by size:
448 138 86
EcoO109I rG'GnC_Cy
Cuts at: 0 40 41 86 318 412 432 457 458
Size: 40 1 45 232 94 20 25 1
Cuts at: 458 479 597 672
Size: 21 118 75
Fragments arranged by size:
232 118 94 75 45 40 25 21
20 1 1
HaeII r_GCGC'y
Cuts at: 0 225 273 672
Size: 225 48 399
Fragments arranged by size:
399 225 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 570 602 672
Size: 570 32 70
Fragments arranged by size:
570 70 32
HincII GTy'rAC
Cuts at: 0 8 672
Size: 8 664
KasI G'GCGC_C
Cuts at: 0 221 672
Size: 221 451
MscI TGG'CCA
Cuts at: 0 115 672
Size: 115 557
MslI CAynn'nnrTG
Cuts at: 0 148 214 337 672
Size: 148 66 123 335
Fragments arranged by size:
335 148 123 66
MspA1I CmG'CkG
Cuts at: 0 148 570 672
Size: 148 422 102
Fragments arranged by size:
422 148 102
NarI GG'CG_CC
Cuts at: 0 222 672
Size: 222 450
NcoI C'CATG_G
Cuts at: 0 64 251 672
Size: 64 187 421
Fragments arranged by size:
421 187 64
PasI CC'CwG_GG
Cuts at: 0 467 672
Size: 467 205
PpuMI rG'GwC_Cy
Cuts at: 0 432 672
Size: 432 240
PspOMI G'GGCC_C
Cuts at: 0 40 85 317 411 457 672
Size: 40 45 232 94 46 215
Fragments arranged by size:
232 215 94 46 45 40
SapI GCTCTTCn'nnn_
Cuts at: 0 136 672
Size: 136 536
SfoI GGC'GCC
Cuts at: 0 223 672
Size: 223 449
StuI AGG'CCT
Cuts at: 0 494 672
Size: 494 178
StyI C'CwwG_G
Cuts at: 0 64 251 617 672
Size: 64 187 366 55
Fragments arranged by size:
366 187 64 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 349 672
Size: 222 127 323
Fragments arranged by size:
323 222 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 577 609 672
Size: 577 32 63
Fragments arranged by size:
577 63 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 672
Size: 150 522
Enzymes that do cut:
AarI AfeI AleI AloI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbvCI BglI BglII Bme1580I
BplI BpmI Bpu10I BsaHI BseRI BseYI BsiHKAI Bsp1286I
BspMI BstEII BstXI BstYI BtgI BtsI EaeI EarI
EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MscI
MslI MspA1I NarI NcoI PasI PpuMI PspOMI SapI
SfoI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI AscI AseI AsiSI AvrII BaeI
BbsI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI
BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI