Restriction map for
pF1KB7106
(Linear) (Six-base) MAPSORT of: KIBB7106.seq Check: 4948 from: 1 to: 678
KIBB7106.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AfeI AGC'GCT
Cuts at: 0 263 678
Size: 263 415
AflIII A'CryG_T
Cuts at: 0 185 678
Size: 185 493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 678
Size: 181 497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 678
Size: 31 32 615
Fragments arranged by size:
615 32 31
AlwNI CAG_nnn'CTG
Cuts at: 0 495 595 678
Size: 495 100 83
Fragments arranged by size:
495 100 83
ApaI G_GGCC'C
Cuts at: 0 234 678
Size: 234 444
ApoI r'AATT_y
Cuts at: 0 340 599 678
Size: 340 259 79
Fragments arranged by size:
340 259 79
BanII G_rGCy'C
Cuts at: 0 234 678
Size: 234 444
BbsI GAAGACnn'nnnn_
Cuts at: 0 450 561 568 678
Size: 450 111 7 110
Fragments arranged by size:
450 111 110 7
BglII A'GATC_T
Cuts at: 0 65 678
Size: 65 613
Bme1580I G_kGCm'C
Cuts at: 0 13 234 678
Size: 13 221 444
Fragments arranged by size:
444 221 13
BsaAI yAC'GTr
Cuts at: 0 282 678
Size: 282 396
BsaWI w'CCGG_w
Cuts at: 0 290 678
Size: 290 388
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 466 496 678
Size: 31 30 405 30 182
Fragments arranged by size:
405 182 31 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 678
Size: 370 308
Bsp1286I G_dGCh'C
Cuts at: 0 13 234 678
Size: 13 221 444
Fragments arranged by size:
444 221 13
BspHI T'CATG_A
Cuts at: 0 581 678
Size: 581 97
BstBI TT'CG_AA
Cuts at: 0 437 678
Size: 437 241
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 531 678
Size: 282 249 147
Fragments arranged by size:
282 249 147
BstYI r'GATC_y
Cuts at: 0 65 678
Size: 65 613
BtgI C'CryG_G
Cuts at: 0 554 678
Size: 554 124
BtsI GCAGTG_nn'
Cuts at: 0 596 678
Size: 596 82
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 39 74 678
Size: 39 35 604
Fragments arranged by size:
604 39 35
EaeI y'GGCC_r
Cuts at: 0 272 678
Size: 272 406
EarI CTCTTCn'nnn_
Cuts at: 0 88 301 678
Size: 88 213 377
Fragments arranged by size:
377 213 88
EcoO109I rG'GnC_Cy
Cuts at: 0 231 678
Size: 231 447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 585 678
Size: 585 93
HaeII r_GCGC'y
Cuts at: 0 265 678
Size: 265 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 678
Size: 466 32 180
Fragments arranged by size:
466 180 32
MscI TGG'CCA
Cuts at: 0 274 678
Size: 274 404
MslI CAynn'nnrTG
Cuts at: 0 280 678
Size: 280 398
MspA1I CmG'CkG
Cuts at: 0 240 492 678
Size: 240 252 186
Fragments arranged by size:
252 240 186
PmlI CAC'GTG
Cuts at: 0 282 678
Size: 282 396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 678
Size: 31 32 615
Fragments arranged by size:
615 32 31
PspOMI G'GGCC_C
Cuts at: 0 230 678
Size: 230 448
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 175 207 678
Size: 175 32 471
Fragments arranged by size:
471 175 32
PstI C_TGCA'G
Cuts at: 0 20 170 239 678
Size: 20 150 69 439
Fragments arranged by size:
439 150 69 20
PvuII CAG'CTG
Cuts at: 0 240 678
Size: 240 438
SfcI C'TryA_G
Cuts at: 0 16 166 235 678
Size: 16 150 69 443
Fragments arranged by size:
443 150 69 16
Tth111I GACn'n_nGTC
Cuts at: 0 151 678
Size: 151 527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 678
Size: 492 186
Enzymes that do cut:
AfeI AflIII AlfI AloI AlwNI ApaI ApoI BanII
BbsI BglII Bme1580I BsaAI BsaWI BsaXI BseRI Bsp1286I
BspHI BstBI BstXI BstYI BtgI BtsI CspCI EaeI
EarI EcoO109I FalI HaeII Hin4I MscI MslI MspA1I
PmlI PpiI PspOMI PsrI PstI PvuII SfcI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpuMI
PshAI PsiI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI XbaI XhoI
XmaI XmnI ZraI