(Linear) (Six-base) MAPSORT of: KIBB7107.seq  Check: 6350  from: 1  to: 687



KIBB7107.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0     193     421     649     687 
   Size:        193     228     228      38

  Fragments arranged by size: 

                 228     228     193      38
 
BanII G_rGCy'C

Cuts at:      0      56     284     512     687 
   Size:         56     228     228     175

  Fragments arranged by size: 

                 228     228     175      56
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     250     478     687 
   Size:        250     228     209

  Fragments arranged by size: 

                 250     228     209
 
BglII A'GATC_T

Cuts at:      0       5     233     461     687 
   Size:          5     228     228     226

  Fragments arranged by size: 

                 228     228     226       5
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      50     278     506     687 
   Size:         50     228     228     181

  Fragments arranged by size: 

                 228     228     181      50
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     687 
   Size:        520     167
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     687 
   Size:         64     623
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     412     640     687 
   Size:        184     228     228      47

  Fragments arranged by size: 

                 228     228     184      47
 
BsmI GAATG_Cn'

Cuts at:      0     104     687 
   Size:        104     583
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     284     512     687 
   Size:         56     228     228     175

  Fragments arranged by size: 

                 228     228     175      56
 
BsrFI r'CCGG_y

Cuts at:      0      25     253     481     687 
   Size:         25     228     228     206

  Fragments arranged by size: 

                 228     228     206      25
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     270     498     687 
   Size:        270     228     189

  Fragments arranged by size: 

                 270     228     189
 
BstYI r'GATC_y

Cuts at:      0       5      86     233     314     461     542     687 
   Size:          5      81     147      81     147      81     145

  Fragments arranged by size: 

                 147     147     145      81      81      81       5
 
Bsu36I CC'TnA_GG

Cuts at:      0     673     687 
   Size:        673      14
 
EaeI y'GGCC_r

Cuts at:      0     156     384     612     687 
   Size:        156     228     228      75

  Fragments arranged by size: 

                 228     228     156      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     687 
   Size:        520     167
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     480     512     687 
   Size:        480      32     175

  Fragments arranged by size: 

                 480     175      32
 
HincII GTy'rAC

Cuts at:      0     194     422     650     687 
   Size:        194     228     228      37

  Fragments arranged by size: 

                 228     228     194      37
 
MspA1I CmG'CkG

Cuts at:      0     147     375     603     687 
   Size:        147     228     228      84

  Fragments arranged by size: 

                 228     228     147      84
 
PfoI T'CCnGG_A

Cuts at:      0      89     317     687 
   Size:         89     228     370

  Fragments arranged by size: 

                 370     228      89
 
PvuII CAG'CTG

Cuts at:      0     147     375     603     687 
   Size:        147     228     228      84

  Fragments arranged by size: 

                 228     228     147      84
 
SalI G'TCGA_C

Cuts at:      0     192     420     648     687 
   Size:        192     228     228      39

  Fragments arranged by size: 

                 228     228     192      39
 
SexAI A'CCwGG_T

Cuts at:      0     203     431     659     687 
   Size:        203     228     228      28

  Fragments arranged by size: 

                 228     228     203      28
 
SmlI C'TyrA_G

Cuts at:      0      43     687 
   Size:         43     644
 
XmnI GAAnn'nnTTC

Cuts at:      0     104     687 
   Size:        104     583
 

 Enzymes that do cut:

     AccI    BanII     BbsI    BglII     BmrI     BpmI    BpuEI     BsgI
     BsmI Bsp1286I    BsrFI    BstXI    BstYI   Bsu36I     EaeI  Eco57MI
    Hin4I   HincII   MspA1I     PfoI    PvuII     SalI    SexAI     SmlI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI