Restriction map for
pF1KB7110
(Linear) (Six-base) MAPSORT of: KIBB7110.seq Check: 2165 from: 1 to: 780
KIBB7110.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 780
Size: 429 351
AlwNI CAG_nnn'CTG
Cuts at: 0 273 780
Size: 273 507
AseI AT'TA_AT
Cuts at: 0 766 780
Size: 766 14
BanI G'GyrC_C
Cuts at: 0 88 387 630 780
Size: 88 299 243 150
Fragments arranged by size:
299 243 150 88
BanII G_rGCy'C
Cuts at: 0 308 780
Size: 308 472
BbeI G_GCGC'C
Cuts at: 0 92 780
Size: 92 688
BbsI GAAGACnn'nnnn_
Cuts at: 0 420 657 780
Size: 420 237 123
Fragments arranged by size:
420 237 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 341 780
Size: 307 34 439
Fragments arranged by size:
439 307 34
BglII A'GATC_T
Cuts at: 0 395 780
Size: 395 385
Bme1580I G_kGCm'C
Cuts at: 0 635 780
Size: 635 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 780
Size: 570 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 598 780
Size: 451 147 182
Fragments arranged by size:
451 182 147
BsaHI Gr'CG_yC
Cuts at: 0 89 780
Size: 89 691
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 780
Size: 391 30 359
Fragments arranged by size:
391 359 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 399 504 604 607 780
Size: 399 105 100 3 173
Fragments arranged by size:
399 173 105 100 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 379 780
Size: 286 93 401
Fragments arranged by size:
401 286 93
BsiHKAI G_wGCw'C
Cuts at: 0 308 780
Size: 308 472
BsmI GAATG_Cn'
Cuts at: 0 330 780
Size: 330 450
Bsp1286I G_dGCh'C
Cuts at: 0 308 635 780
Size: 308 327 145
Fragments arranged by size:
327 308 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 215 780
Size: 215 565
BstAPI GCAn_nnn'nTGC
Cuts at: 0 455 780
Size: 455 325
BstYI r'GATC_y
Cuts at: 0 395 780
Size: 395 385
Bsu36I CC'TnA_GG
Cuts at: 0 212 780
Size: 212 568
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 713 780
Size: 713 67
ClaI AT'CG_AT
Cuts at: 0 713 780
Size: 713 67
EaeI y'GGCC_r
Cuts at: 0 566 780
Size: 566 214
EarI CTCTTCn'nnn_
Cuts at: 0 417 466 493 668 780
Size: 417 49 27 175 112
Fragments arranged by size:
417 175 112 49 27
EcoICRI GAG'CTC
Cuts at: 0 306 780
Size: 306 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 780
Size: 429 351
EcoNI CCTnn'n_nnAGG
Cuts at: 0 211 277 586 780
Size: 211 66 309 194
Fragments arranged by size:
309 211 194 66
EcoO109I rG'GnC_Cy
Cuts at: 0 282 537 579 780
Size: 282 255 42 201
Fragments arranged by size:
282 255 201 42
HaeII r_GCGC'y
Cuts at: 0 92 780
Size: 92 688
KasI G'GCGC_C
Cuts at: 0 88 780
Size: 88 692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 780
Size: 29 751
MscI TGG'CCA
Cuts at: 0 568 780
Size: 568 212
MslI CAynn'nnrTG
Cuts at: 0 728 780
Size: 728 52
MspA1I CmG'CkG
Cuts at: 0 101 780
Size: 101 679
NarI GG'CG_CC
Cuts at: 0 89 780
Size: 89 691
NspI r_CATG'y
Cuts at: 0 733 780
Size: 733 47
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 780
Size: 228 552
PfoI T'CCnGG_A
Cuts at: 0 221 780
Size: 221 559
PpuMI rG'GwC_Cy
Cuts at: 0 537 780
Size: 537 243
PstI C_TGCA'G
Cuts at: 0 290 780
Size: 290 490
SacI G_AGCT'C
Cuts at: 0 308 780
Size: 308 472
SapI GCTCTTCn'nnn_
Cuts at: 0 493 780
Size: 493 287
SbfI CC_TGCA'GG
Cuts at: 0 290 780
Size: 290 490
SexAI A'CCwGG_T
Cuts at: 0 260 780
Size: 260 520
SfcI C'TryA_G
Cuts at: 0 286 399 780
Size: 286 113 381
Fragments arranged by size:
381 286 113
SfoI GGC'GCC
Cuts at: 0 90 780
Size: 90 690
SmlI C'TyrA_G
Cuts at: 0 466 613 780
Size: 466 147 167
Fragments arranged by size:
466 167 147
StyI C'CwwG_G
Cuts at: 0 311 474 780
Size: 311 163 306
Fragments arranged by size:
311 306 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 552 615 740 780
Size: 552 63 125 40
Fragments arranged by size:
552 125 63 40
TatI w'GTAC_w
Cuts at: 0 596 780
Size: 596 184
Enzymes that do cut:
AcuI AlwNI AseI BanI BanII BbeI BbsI BcgI
BglII Bme1580I BmrI BpuEI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspMI BstAPI BstYI Bsu36I BtgZI
ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
KasI MmeI MscI MslI MspA1I NarI NspI PflMI
PfoI PpuMI PstI SacI SapI SbfI SexAI SfcI
SfoI SmlI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgI BtsI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI