Restriction map for
pF1KB7111
(Linear) (Six-base) MAPSORT of: KIBB7111.seq Check: 4978 from: 1 to: 783
KIBB7111.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 494 783
Size: 494 289
Acc65I G'GTAC_C
Cuts at: 0 479 783
Size: 479 304
AfeI AGC'GCT
Cuts at: 0 263 783
Size: 263 520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 645 783
Size: 181 464 138
Fragments arranged by size:
464 181 138
ApaI G_GGCC'C
Cuts at: 0 234 783
Size: 234 549
BanI G'GyrC_C
Cuts at: 0 479 783
Size: 479 304
BanII G_rGCy'C
Cuts at: 0 234 783
Size: 234 549
BbsI GAAGACnn'nnnn_
Cuts at: 0 568 783
Size: 568 215
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 392 783
Size: 358 34 391
Fragments arranged by size:
391 358 34
BfrBI ATG'CAT
Cuts at: 0 279 783
Size: 279 504
Bme1580I G_kGCm'C
Cuts at: 0 234 783
Size: 234 549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 783
Size: 220 563
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 783
Size: 31 30 722
Fragments arranged by size:
722 31 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 338 783
Size: 338 445
BseYI C'CCAG_C
Cuts at: 0 258 783
Size: 258 525
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 783
Size: 1 782
BsiHKAI G_wGCw'C
Cuts at: 0 675 783
Size: 675 108
BsmBI CGTCTCn'nnnn_
Cuts at: 0 255 783
Size: 255 528
Bsp1286I G_dGCh'C
Cuts at: 0 234 675 783
Size: 234 441 108
Fragments arranged by size:
441 234 108
BspHI T'CATG_A
Cuts at: 0 98 783
Size: 98 685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 644 783
Size: 494 150 139
Fragments arranged by size:
494 150 139
BssSI C'ACGA_G
Cuts at: 0 106 783
Size: 106 677
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 783
Size: 282 501
BtsI GCAGTG_nn'
Cuts at: 0 163 783
Size: 163 620
EaeI y'GGCC_r
Cuts at: 0 272 783
Size: 272 511
EarI CTCTTCn'nnn_
Cuts at: 0 325 783
Size: 325 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 783
Size: 220 563
EcoNI CCTnn'n_nnAGG
Cuts at: 0 291 783
Size: 291 492
EcoO109I rG'GnC_Cy
Cuts at: 0 231 783
Size: 231 552
HaeII r_GCGC'y
Cuts at: 0 265 783
Size: 265 518
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 86 118 783
Size: 86 32 665
Fragments arranged by size:
665 86 32
HincII GTy'rAC
Cuts at: 0 39 534 783
Size: 39 495 249
Fragments arranged by size:
495 249 39
KpnI G_GTAC'C
Cuts at: 0 483 783
Size: 483 300
MscI TGG'CCA
Cuts at: 0 274 783
Size: 274 509
MslI CAynn'nnrTG
Cuts at: 0 280 580 783
Size: 280 300 203
Fragments arranged by size:
300 280 203
MspA1I CmG'CkG
Cuts at: 0 240 783
Size: 240 543
NsiI A_TGCA'T
Cuts at: 0 281 783
Size: 281 502
NspI r_CATG'y
Cuts at: 0 283 609 783
Size: 283 326 174
Fragments arranged by size:
326 283 174
PasI CC'CwG_GG
Cuts at: 0 491 783
Size: 491 292
PspOMI G'GGCC_C
Cuts at: 0 230 783
Size: 230 553
PvuII CAG'CTG
Cuts at: 0 240 783
Size: 240 543
SfcI C'TryA_G
Cuts at: 0 291 783
Size: 291 492
SphI G_CATG'C
Cuts at: 0 609 783
Size: 609 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 483 783
Size: 483 300
Tth111I GACn'n_nGTC
Cuts at: 0 151 783
Size: 151 632
XmnI GAAnn'nnTTC
Cuts at: 0 340 783
Size: 340 443
Enzymes that do cut:
AarI Acc65I AfeI AlfI ApaI BanI BanII BbsI
BcgI BfrBI Bme1580I BpmI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BssSI BstXI BtsI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KpnI MscI MslI MspA1I NsiI NspI PasI PspOMI
PvuII SfcI SphI TaqII Tth111I XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI StuI StyI SwaI TatI
TstI XbaI XcmI XhoI XmaI ZraI