Restriction map for
pF1KB7113
(Linear) (Six-base) MAPSORT of: KIBB7113.seq Check: 8432 from: 1 to: 810
KIBB7113.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 810
Size: 721 89
AccI GT'mk_AC
Cuts at: 0 104 810
Size: 104 706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 810
Size: 770 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 810
Size: 31 32 747
Fragments arranged by size:
747 32 31
ApaI G_GGCC'C
Cuts at: 0 234 810
Size: 234 576
AvaI C'yCGr_G
Cuts at: 0 668 810
Size: 668 142
BamHI G'GATC_C
Cuts at: 0 753 810
Size: 753 57
BanI G'GyrC_C
Cuts at: 0 311 577 810
Size: 311 266 233
Fragments arranged by size:
311 266 233
BanII G_rGCy'C
Cuts at: 0 234 617 810
Size: 234 383 193
Fragments arranged by size:
383 234 193
BbeI G_GCGC'C
Cuts at: 0 581 810
Size: 581 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 16 81 810
Size: 16 65 729
Fragments arranged by size:
729 65 16
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 659 693 810
Size: 659 34 117
Fragments arranged by size:
659 117 34
BglI GCCn_nnn'nGGC
Cuts at: 0 499 810
Size: 499 311
Bme1580I G_kGCm'C
Cuts at: 0 234 810
Size: 234 576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 810
Size: 1 809
BsaAI yAC'GTr
Cuts at: 0 108 282 810
Size: 108 174 528
Fragments arranged by size:
528 174 108
BsaHI Gr'CG_yC
Cuts at: 0 578 810
Size: 578 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 810
Size: 31 30 749
Fragments arranged by size:
749 31 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 810
Size: 310 500
BseYI C'CCAG_C
Cuts at: 0 742 810
Size: 742 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 262 430 505 637 810
Size: 162 100 168 75 132 173
Fragments arranged by size:
173 168 162 132 100 75
BsiHKAI G_wGCw'C
Cuts at: 0 617 810
Size: 617 193
Bsp1286I G_dGCh'C
Cuts at: 0 234 617 810
Size: 234 383 193
Fragments arranged by size:
383 234 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 721 810
Size: 449 272 89
Fragments arranged by size:
449 272 89
BsrBI CCG'CTC
Cuts at: 0 32 300 810
Size: 32 268 510
Fragments arranged by size:
510 268 32
BsrDI GCAATG_nn'
Cuts at: 0 530 810
Size: 530 280
BsrFI r'CCGG_y
Cuts at: 0 308 810
Size: 308 502
BstXI CCAn_nnnn'nTGG
Cuts at: 0 519 810
Size: 519 291
BstYI r'GATC_y
Cuts at: 0 753 810
Size: 753 57
BstZ17I GTA'TAC
Cuts at: 0 105 810
Size: 105 705
BtgI C'CryG_G
Cuts at: 0 77 810
Size: 77 733
BtsI GCAGTG_nn'
Cuts at: 0 493 810
Size: 493 317
EarI CTCTTCn'nnn_
Cuts at: 0 436 810
Size: 436 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 573 684 810
Size: 211 362 111 126
Fragments arranged by size:
362 211 126 111
EcoICRI GAG'CTC
Cuts at: 0 615 810
Size: 615 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 770 810
Size: 1 769 40
Fragments arranged by size:
769 40 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 655 810
Size: 655 155
EcoO109I rG'GnC_Cy
Cuts at: 0 21 231 639 810
Size: 21 210 408 171
Fragments arranged by size:
408 210 171 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 573 810
Size: 573 237
HaeII r_GCGC'y
Cuts at: 0 309 581 810
Size: 309 272 229
Fragments arranged by size:
309 272 229
KasI G'GCGC_C
Cuts at: 0 577 810
Size: 577 233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 810
Size: 239 571
MspA1I CmG'CkG
Cuts at: 0 719 810
Size: 719 91
NaeI GCC'GGC
Cuts at: 0 310 810
Size: 310 500
NarI GG'CG_CC
Cuts at: 0 578 810
Size: 578 232
NgoMIV G'CCGG_C
Cuts at: 0 308 810
Size: 308 502
NspI r_CATG'y
Cuts at: 0 257 810
Size: 257 553
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 810
Size: 732 78
PmlI CAC'GTG
Cuts at: 0 282 810
Size: 282 528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 810
Size: 31 32 747
Fragments arranged by size:
747 32 31
PpuMI rG'GwC_Cy
Cuts at: 0 639 810
Size: 639 171
PspOMI G'GGCC_C
Cuts at: 0 230 810
Size: 230 580
PspXI vC'TCGA_Gb
Cuts at: 0 668 810
Size: 668 142
PstI C_TGCA'G
Cuts at: 0 120 239 782 810
Size: 120 119 543 28
Fragments arranged by size:
543 120 119 28
SacI G_AGCT'C
Cuts at: 0 617 810
Size: 617 193
ScaI AGT'ACT
Cuts at: 0 46 810
Size: 46 764
SfcI C'TryA_G
Cuts at: 0 116 235 778 810
Size: 116 119 543 32
Fragments arranged by size:
543 119 116 32
SfoI GGC'GCC
Cuts at: 0 579 810
Size: 579 231
SmlI C'TyrA_G
Cuts at: 0 668 810
Size: 668 142
StuI AGG'CCT
Cuts at: 0 377 650 810
Size: 377 273 160
Fragments arranged by size:
377 273 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 807 810
Size: 807 3
TatI w'GTAC_w
Cuts at: 0 44 810
Size: 44 766
XhoI C'TCGA_G
Cuts at: 0 668 810
Size: 668 142
XmnI GAAnn'nnTTC
Cuts at: 0 556 810
Size: 556 254
Enzymes that do cut:
AarI AccI AcuI AloI ApaI AvaI BamHI BanI
BanII BbeI BbsI BcgI BglI Bme1580I BpmI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstXI BstYI BstZ17I BtgI BtsI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
KasI MmeI MspA1I NaeI NarI NgoMIV NspI PflMI
PmlI PpiI PpuMI PspOMI PspXI PstI SacI ScaI
SfcI SfoI SmlI StuI TaqII TatI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PshAI
PsiI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TstI Tth111I
XbaI XcmI XmaI ZraI