Restriction map for
pF1KB7114
(Linear) (Six-base) MAPSORT of: KIBB7114.seq Check: 5425 from: 1 to: 819
KIBB7114.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
Acc65I G'GTAC_C
Cuts at: 0 697 819
Size: 697 122
ApaI G_GGCC'C
Cuts at: 0 234 819
Size: 234 585
ApaLI G'TGCA_C
Cuts at: 0 675 819
Size: 675 144
AvaI C'yCGr_G
Cuts at: 0 791 819
Size: 791 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 27 819
Size: 27 792
BanI G'GyrC_C
Cuts at: 0 311 341 697 819
Size: 311 30 356 122
Fragments arranged by size:
356 311 122 30
BanII G_rGCy'C
Cuts at: 0 234 380 614 757 819
Size: 234 146 234 143 62
Fragments arranged by size:
234 234 146 143 62
BbeI G_GCGC'C
Cuts at: 0 345 819
Size: 345 474
BbsI GAAGACnn'nnnn_
Cuts at: 0 75 819
Size: 75 744
BglI GCCn_nnn'nGGC
Cuts at: 0 173 819
Size: 173 646
Bme1580I G_kGCm'C
Cuts at: 0 234 679 819
Size: 234 445 140
Fragments arranged by size:
445 234 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 819
Size: 27 792
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 819
Size: 650 169
BsaI GGTCTCn'nnnn_
Cuts at: 0 788 819
Size: 788 31
BsaAI yAC'GTr
Cuts at: 0 282 819
Size: 282 537
BsaHI Gr'CG_yC
Cuts at: 0 342 819
Size: 342 477
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 819
Size: 31 30 758
Fragments arranged by size:
758 31 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 761 819
Size: 761 58
BsiEI CG_ry'CG
Cuts at: 0 119 819
Size: 119 700
BsiHKAI G_wGCw'C
Cuts at: 0 614 679 819
Size: 614 65 140
Fragments arranged by size:
614 140 65
Bsp1286I G_dGCh'C
Cuts at: 0 234 380 614 679 757 819
Size: 234 146 234 65 78 62
Fragments arranged by size:
234 234 146 78 65 62
BssSI C'ACGA_G
Cuts at: 0 268 604 640 819
Size: 268 336 36 179
Fragments arranged by size:
336 268 179 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 635 819
Size: 635 184
BstEII G'GTnAC_C
Cuts at: 0 736 819
Size: 736 83
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 819
Size: 516 303
BstYI r'GATC_y
Cuts at: 0 498 819
Size: 498 321
BtgI C'CryG_G
Cuts at: 0 324 693 819
Size: 324 369 126
Fragments arranged by size:
369 324 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 819
Size: 411 408
BtsI GCAGTG_nn'
Cuts at: 0 262 819
Size: 262 557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 39 74 819
Size: 39 35 745
Fragments arranged by size:
745 39 35
EaeI y'GGCC_r
Cuts at: 0 116 572 819
Size: 116 456 247
Fragments arranged by size:
456 247 116
EagI C'GGCC_G
Cuts at: 0 116 819
Size: 116 703
EarI CTCTTCn'nnn_
Cuts at: 0 301 568 711 819
Size: 301 267 143 108
Fragments arranged by size:
301 267 143 108
EcoICRI GAG'CTC
Cuts at: 0 612 819
Size: 612 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 819
Size: 27 792
EcoO109I rG'GnC_Cy
Cuts at: 0 231 819
Size: 231 588
HaeII r_GCGC'y
Cuts at: 0 345 819
Size: 345 474
HincII GTy'rAC
Cuts at: 0 39 819
Size: 39 780
KasI G'GCGC_C
Cuts at: 0 341 819
Size: 341 478
KpnI G_GTAC'C
Cuts at: 0 701 819
Size: 701 118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 819
Size: 206 613
MscI TGG'CCA
Cuts at: 0 574 819
Size: 574 245
MslI CAynn'nnrTG
Cuts at: 0 280 819
Size: 280 539
MspA1I CmG'CkG
Cuts at: 0 580 819
Size: 580 239
NarI GG'CG_CC
Cuts at: 0 342 819
Size: 342 477
NcoI C'CATG_G
Cuts at: 0 324 819
Size: 324 495
NdeI CA'TA_TG
Cuts at: 0 390 819
Size: 390 429
NspI r_CATG'y
Cuts at: 0 257 819
Size: 257 562
PmlI CAC'GTG
Cuts at: 0 282 819
Size: 282 537
PshAI GACnn'nnGTC
Cuts at: 0 391 819
Size: 391 428
PspOMI G'GGCC_C
Cuts at: 0 230 819
Size: 230 589
PstI C_TGCA'G
Cuts at: 0 239 400 819
Size: 239 161 419
Fragments arranged by size:
419 239 161
PvuII CAG'CTG
Cuts at: 0 580 819
Size: 580 239
SacI G_AGCT'C
Cuts at: 0 614 819
Size: 614 205
ScaI AGT'ACT
Cuts at: 0 46 819
Size: 46 773
SexAI A'CCwGG_T
Cuts at: 0 107 620 819
Size: 107 513 199
Fragments arranged by size:
513 199 107
SfcI C'TryA_G
Cuts at: 0 235 396 819
Size: 235 161 423
Fragments arranged by size:
423 235 161
SfoI GGC'GCC
Cuts at: 0 343 819
Size: 343 476
SmlI C'TyrA_G
Cuts at: 0 665 819
Size: 665 154
StyI C'CwwG_G
Cuts at: 0 324 819
Size: 324 495
TatI w'GTAC_w
Cuts at: 0 44 819
Size: 44 775
XmnI GAAnn'nnTTC
Cuts at: 0 553 819
Size: 553 266
Enzymes that do cut:
Acc65I ApaI ApaLI AvaI BaeI BanI BanII BbeI
BbsI BglI Bme1580I BpmI BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BsiEI BsiHKAI Bsp1286I BssSI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EagI
EarI EcoICRI Eco57MI EcoO109I HaeII HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NdeI NspI
PmlI PshAI PspOMI PstI PvuII SacI ScaI SexAI
SfcI SfoI SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI
AseI AsiSI AvrII BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaBI BsaWI BseYI BsgI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstBI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI MfeI MluI NaeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI