Restriction map for
pF1KB7119
(Linear) (Six-base) MAPSORT of: KIBB7119.seq Check: 6084 from: 1 to: 861
KIBB7119.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 774 861
Size: 774 87
AccI GT'mk_AC
Cuts at: 0 758 861
Size: 758 103
AflIII A'CryG_T
Cuts at: 0 536 560 861
Size: 536 24 301
Fragments arranged by size:
536 301 24
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 861
Size: 85 32 744
Fragments arranged by size:
744 85 32
AlwNI CAG_nnn'CTG
Cuts at: 0 171 861
Size: 171 690
AvaI C'yCGr_G
Cuts at: 0 284 516 861
Size: 284 232 345
Fragments arranged by size:
345 284 232
BanI G'GyrC_C
Cuts at: 0 230 861
Size: 230 631
BanII G_rGCy'C
Cuts at: 0 680 740 861
Size: 680 60 121
Fragments arranged by size:
680 121 60
BbeI G_GCGC'C
Cuts at: 0 234 861
Size: 234 627
BbvCI CC'TCA_GC
Cuts at: 0 235 341 861
Size: 235 106 520
Fragments arranged by size:
520 235 106
BglI GCCn_nnn'nGGC
Cuts at: 0 280 861
Size: 280 581
BlpI GC'TnA_GC
Cuts at: 0 328 861
Size: 328 533
BmgBI CAC'GTC
Cuts at: 0 183 221 561 588 861
Size: 183 38 340 27 273
Fragments arranged by size:
340 273 183 38 27
BmtI G_CTAG'C
Cuts at: 0 16 861
Size: 16 845
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 861
Size: 96 765
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 514 670 861
Size: 142 372 156 191
Fragments arranged by size:
372 191 156 142
Bpu10I CC'TnA_GC
Cuts at: 0 235 341 861
Size: 235 106 520
Fragments arranged by size:
520 235 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 861
Size: 841 20
BsaBI GATnn'nnATC
Cuts at: 0 478 861
Size: 478 383
BsaHI Gr'CG_yC
Cuts at: 0 231 861
Size: 231 630
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 244 274 861
Size: 115 30 99 30 587
Fragments arranged by size:
587 115 99 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 823 861
Size: 823 38
BseYI C'CCAG_C
Cuts at: 0 192 347 861
Size: 192 155 514
Fragments arranged by size:
514 192 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 861
Size: 820 41
BsiEI CG_ry'CG
Cuts at: 0 758 861
Size: 758 103
BsiHKAI G_wGCw'C
Cuts at: 0 680 740 861
Size: 680 60 121
Fragments arranged by size:
680 121 60
BsmBI CGTCTCn'nnnn_
Cuts at: 0 100 861
Size: 100 761
Bsp1286I G_dGCh'C
Cuts at: 0 680 740 861
Size: 680 60 121
Fragments arranged by size:
680 121 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 774 861
Size: 774 87
BsrFI r'CCGG_y
Cuts at: 0 8 766 861
Size: 8 758 95
Fragments arranged by size:
758 95 8
BssHII G'CGCG_C
Cuts at: 0 312 373 420 596 861
Size: 312 61 47 176 265
Fragments arranged by size:
312 265 176 61 47
BssSI C'ACGA_G
Cuts at: 0 88 673 861
Size: 88 585 188
Fragments arranged by size:
585 188 88
BstYI r'GATC_y
Cuts at: 0 473 861
Size: 473 388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 249 861
Size: 249 612
BtsI GCAGTG_nn'
Cuts at: 0 300 374 861
Size: 300 74 487
Fragments arranged by size:
487 300 74
EaeI y'GGCC_r
Cuts at: 0 176 581 768 861
Size: 176 405 187 93
Fragments arranged by size:
405 187 176 93
EarI CTCTTCn'nnn_
Cuts at: 0 685 861
Size: 685 176
EcoICRI GAG'CTC
Cuts at: 0 678 738 861
Size: 678 60 123
Fragments arranged by size:
678 123 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 514 670 861
Size: 142 372 156 191
Fragments arranged by size:
372 191 156 142
HaeII r_GCGC'y
Cuts at: 0 234 489 861
Size: 234 255 372
Fragments arranged by size:
372 255 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 861
Size: 244 32 585
Fragments arranged by size:
585 244 32
HincII GTy'rAC
Cuts at: 0 759 861
Size: 759 102
KasI G'GCGC_C
Cuts at: 0 230 861
Size: 230 631
MslI CAynn'nnrTG
Cuts at: 0 223 861
Size: 223 638
MspA1I CmG'CkG
Cuts at: 0 168 492 861
Size: 168 324 369
Fragments arranged by size:
369 324 168
NarI GG'CG_CC
Cuts at: 0 231 861
Size: 231 630
NheI G'CTAG_C
Cuts at: 0 12 861
Size: 12 849
NspI r_CATG'y
Cuts at: 0 540 861
Size: 540 321
PciI A'CATG_T
Cuts at: 0 536 861
Size: 536 325
PflMI CCAn_nnn'nTGG
Cuts at: 0 120 171 861
Size: 120 51 690
Fragments arranged by size:
690 120 51
PfoI T'CCnGG_A
Cuts at: 0 119 143 861
Size: 119 24 718
Fragments arranged by size:
718 119 24
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 861
Size: 523 32 306
Fragments arranged by size:
523 306 32
PvuII CAG'CTG
Cuts at: 0 168 492 861
Size: 168 324 369
Fragments arranged by size:
369 324 168
SacI G_AGCT'C
Cuts at: 0 680 740 861
Size: 680 60 121
Fragments arranged by size:
680 121 60
SalI G'TCGA_C
Cuts at: 0 757 861
Size: 757 104
SapI GCTCTTCn'nnn_
Cuts at: 0 685 861
Size: 685 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 280 861
Size: 280 581
SfoI GGC'GCC
Cuts at: 0 232 861
Size: 232 629
SmaI CCC'GGG
Cuts at: 0 286 861
Size: 286 575
SmlI C'TyrA_G
Cuts at: 0 856 861
Size: 856 5
StyI C'CwwG_G
Cuts at: 0 269 776 861
Size: 269 507 85
Fragments arranged by size:
507 269 85
TatI w'GTAC_w
Cuts at: 0 392 861
Size: 392 469
XmaI C'CCGG_G
Cuts at: 0 284 861
Size: 284 577
Enzymes that do cut:
AarI AccI AflIII AloI AlwNI AvaI BanI BanII
BbeI BbvCI BglI BlpI BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI
BstYI BtgZI BtsI EaeI EarI EcoICRI Eco57MI HaeII
Hin4I HincII KasI MslI MspA1I NarI NheI NspI
PciI PflMI PfoI PpiI PvuII SacI SalI SapI
SfiI SfoI SmaI SmlI StyI TatI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BglII Bme1580I BmrI BsaI BsaAI BsaWI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MmeI MscI NaeI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmnI ZraI