(Linear) (Six-base) MAPSORT of: KIBB7120.seq  Check: 3325  from: 1  to: 882



KIBB7120.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AccI GT'mk_AC

Cuts at:      0     258     274     882 
   Size:        258      16     608

  Fragments arranged by size: 

                 608     258      16
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     554     882 
   Size:        362     192     328

  Fragments arranged by size: 

                 362     328     192
 
AflII C'TTAA_G

Cuts at:      0     262     882 
   Size:        262     620
 
ApoI r'AATT_y

Cuts at:      0     208     296     799     882 
   Size:        208      88     503      83

  Fragments arranged by size: 

                 503     208      88      83
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     386     882 
   Size:        386     496
 
BsrDI GCAATG_nn'

Cuts at:      0     422     882 
   Size:        422     460
 
BstYI r'GATC_y

Cuts at:      0     690     882 
   Size:        690     192
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     412     882 
   Size:        412     470
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     554     882 
   Size:        362     192     328

  Fragments arranged by size: 

                 362     328     192
 
EcoRV GAT'ATC

Cuts at:      0     707     882 
   Size:        707     175
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      28     640     851     882 
   Size:         28     612     211      31

  Fragments arranged by size: 

                 612     211      31      28
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     722     754     882 
   Size:        722      32     128

  Fragments arranged by size: 

                 722     128      32
 
HincII GTy'rAC

Cuts at:      0     783     882 
   Size:        783      99
 
HindIII A'AGCT_T

Cuts at:      0     588     882 
   Size:        588     294
 
HpaI GTT'AAC

Cuts at:      0     783     882 
   Size:        783      99
 
MslI CAynn'nnrTG

Cuts at:      0     152     882 
   Size:        152     730
 
NspI r_CATG'y

Cuts at:      0     412     882 
   Size:        412     470
 
ScaI AGT'ACT

Cuts at:      0     845     882 
   Size:        845      37
 
SfcI C'TryA_G

Cuts at:      0      74     103     134     169     870     882 
   Size:         74      29      31      35     701      12

  Fragments arranged by size: 

                 701      74      35      31      29      12
 
SmlI C'TyrA_G

Cuts at:      0     262     882 
   Size:        262     620
 
SphI G_CATG'C

Cuts at:      0     412     882 
   Size:        412     470
 
SspI AAT'ATT

Cuts at:      0     747     882 
   Size:        747     135
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      23     882 
   Size:         23     859
 
TatI w'GTAC_w

Cuts at:      0     766     843     882 
   Size:        766      77      39

  Fragments arranged by size: 

                 766      77      39
 
XbaI T'CTAG_A

Cuts at:      0      52     882 
   Size:         52     830
 

 Enzymes that do cut:

     AccI     AcuI    AflII     ApoI    BciVI    BsrDI    BstYI     EciI
  Eco57MI    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI     MslI
     NspI     ScaI     SfcI     SmlI     SphI     SspI    TaqII     TatI
     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI