Restriction map for
pF1KB7138
(Linear) (Six-base) MAPSORT of: KIBB7138.seq Check: 1726 from: 1 to: 993
KIBB7138.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 520 708 993
Size: 520 188 285
Fragments arranged by size:
520 285 188
AatII G_ACGT'C
Cuts at: 0 658 993
Size: 658 335
AccI GT'mk_AC
Cuts at: 0 957 993
Size: 957 36
AflIII A'CryG_T
Cuts at: 0 401 835 993
Size: 401 434 158
Fragments arranged by size:
434 401 158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 430 993
Size: 430 563
AvaI C'yCGr_G
Cuts at: 0 884 993
Size: 884 109
BanII G_rGCy'C
Cuts at: 0 297 993
Size: 297 696
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 993
Size: 72 921
BbvCI CC'TCA_GC
Cuts at: 0 829 993
Size: 829 164
BclI T'GATC_A
Cuts at: 0 2 993
Size: 2 991
BglI GCCn_nnn'nGGC
Cuts at: 0 418 993
Size: 418 575
Bpu10I CC'TnA_GC
Cuts at: 0 829 993
Size: 829 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 993
Size: 502 491
BsaAI yAC'GTr
Cuts at: 0 404 836 993
Size: 404 432 157
Fragments arranged by size:
432 404 157
BsaBI GATnn'nnATC
Cuts at: 0 700 993
Size: 700 293
BsaHI Gr'CG_yC
Cuts at: 0 655 993
Size: 655 338
BseYI C'CCAG_C
Cuts at: 0 768 993
Size: 768 225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 993
Size: 736 257
BsiHKAI G_wGCw'C
Cuts at: 0 297 725 993
Size: 297 428 268
Fragments arranged by size:
428 297 268
Bsp1286I G_dGCh'C
Cuts at: 0 297 725 993
Size: 297 428 268
Fragments arranged by size:
428 297 268
BspHI T'CATG_A
Cuts at: 0 605 821 993
Size: 605 216 172
Fragments arranged by size:
605 216 172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 520 708 993
Size: 520 188 285
Fragments arranged by size:
520 285 188
BsrDI GCAATG_nn'
Cuts at: 0 317 993
Size: 317 676
BsrGI T'GTAC_A
Cuts at: 0 369 993
Size: 369 624
BstBI TT'CG_AA
Cuts at: 0 231 921 993
Size: 231 690 72
Fragments arranged by size:
690 231 72
BstEII G'GTnAC_C
Cuts at: 0 168 993
Size: 168 825
BstXI CCAn_nnnn'nTGG
Cuts at: 0 289 993
Size: 289 704
BtgI C'CryG_G
Cuts at: 0 164 993
Size: 164 829
BtsI GCAGTG_nn'
Cuts at: 0 122 993
Size: 122 871
EaeI y'GGCC_r
Cuts at: 0 259 362 993
Size: 259 103 631
Fragments arranged by size:
631 259 103
EarI CTCTTCn'nnn_
Cuts at: 0 151 993
Size: 151 842
EcoICRI GAG'CTC
Cuts at: 0 295 993
Size: 295 698
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 338 993
Size: 338 655
FspI TGC'GCA
Cuts at: 0 931 993
Size: 931 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 447 479 481 513 993
Size: 447 32 2 32 480
Fragments arranged by size:
480 447 32 32 2
HincII GTy'rAC
Cuts at: 0 117 993
Size: 117 876
HpaI GTT'AAC
Cuts at: 0 117 993
Size: 117 876
MscI TGG'CCA
Cuts at: 0 261 364 993
Size: 261 103 629
Fragments arranged by size:
629 261 103
MslI CAynn'nnrTG
Cuts at: 0 53 178 993
Size: 53 125 815
Fragments arranged by size:
815 125 53
NspI r_CATG'y
Cuts at: 0 930 993
Size: 930 63
PasI CC'CwG_GG
Cuts at: 0 797 993
Size: 797 196
PmlI CAC'GTG
Cuts at: 0 404 836 993
Size: 404 432 157
Fragments arranged by size:
432 404 157
PsiI TTA'TAA
Cuts at: 0 214 993
Size: 214 779
PspXI vC'TCGA_Gb
Cuts at: 0 884 993
Size: 884 109
SacI G_AGCT'C
Cuts at: 0 297 993
Size: 297 696
ScaI AGT'ACT
Cuts at: 0 274 993
Size: 274 719
SfcI C'TryA_G
Cuts at: 0 84 993
Size: 84 909
SmlI C'TyrA_G
Cuts at: 0 517 884 993
Size: 517 367 109
Fragments arranged by size:
517 367 109
SphI G_CATG'C
Cuts at: 0 930 993
Size: 930 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 602 993
Size: 602 391
TatI w'GTAC_w
Cuts at: 0 272 369 993
Size: 272 97 624
Fragments arranged by size:
624 272 97
XhoI C'TCGA_G
Cuts at: 0 884 993
Size: 884 109
ZraI GAC'GTC
Cuts at: 0 656 993
Size: 656 337
Enzymes that do cut:
AarI AatII AccI AflIII AlfI AvaI BanII BbsI
BbvCI BclI BglI Bpu10I BpuEI BsaAI BsaBI BsaHI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI
BstBI BstEII BstXI BtgI BtsI EaeI EarI EcoICRI
FalI FspI Hin4I HincII HpaI MscI MslI NspI
PasI PmlI PsiI PspXI SacI ScaI SfcI SmlI
SphI TaqII TatI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BsaI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstAPI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspAI
HaeII HindIII KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XcmI XmaI XmnI