(Linear) (Six-base) MAPSORT of: KIBB7140.seq  Check: 1793  from: 1  to: 999



KIBB7140.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     804     999 
   Size:        804     195
 
AleI CACnn'nnGTG

Cuts at:      0      10     193     999 
   Size:         10     183     806

  Fragments arranged by size: 

                 806     183      10
 
ApoI r'AATT_y

Cuts at:      0     904     999 
   Size:        904      95
 
BamHI G'GATC_C

Cuts at:      0     873     999 
   Size:        873     126
 
BanI G'GyrC_C

Cuts at:      0      41     255     999 
   Size:         41     214     744

  Fragments arranged by size: 

                 744     214      41
 
BanII G_rGCy'C

Cuts at:      0     344     999 
   Size:        344     655
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     717     999 
   Size:        717     282
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     482     516     999 
   Size:        482      34     483

  Fragments arranged by size: 

                 483     482      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     540     999 
   Size:        540     459
 
BglII A'GATC_T

Cuts at:      0      31     999 
   Size:         31     968
 
Bme1580I G_kGCm'C

Cuts at:      0      46     999 
   Size:         46     953
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     498     999 
   Size:        349     149     501

  Fragments arranged by size: 

                 501     349     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     304     429     999 
   Size:        304     125     570

  Fragments arranged by size: 

                 570     304     125
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     150     999 
   Size:        150     849
 
BseYI C'CCAG_C

Cuts at:      0      78     154     999 
   Size:         78      76     845

  Fragments arranged by size: 

                 845      78      76
 
Bsp1286I G_dGCh'C

Cuts at:      0      46     344     999 
   Size:         46     298     655

  Fragments arranged by size: 

                 655     298      46
 
BsrBI CCG'CTC

Cuts at:      0      39     999 
   Size:         39     960
 
BsrFI r'CCGG_y

Cuts at:      0     106     999 
   Size:        106     893
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     321     999 
   Size:        321     678
 
BstYI r'GATC_y

Cuts at:      0      31     873     999 
   Size:         31     842     126

  Fragments arranged by size: 

                 842     126      31
 
Bsu36I CC'TnA_GG

Cuts at:      0     993     999 
   Size:        993       6
 
EaeI y'GGCC_r

Cuts at:      0     197     999 
   Size:        197     802
 
EcoO109I rG'GnC_Cy

Cuts at:      0     532     999 
   Size:        532     467
 
MslI CAynn'nnrTG

Cuts at:      0      10      13     193     319     868     999 
   Size:         10       3     180     126     549     131

  Fragments arranged by size: 

                 549     180     131     126      10       3
 
MspA1I CmG'CkG

Cuts at:      0      69     999 
   Size:         69     930
 
NdeI CA'TA_TG

Cuts at:      0     571     999 
   Size:        571     428
 
PvuII CAG'CTG

Cuts at:      0      69     999 
   Size:         69     930
 
SfcI C'TryA_G

Cuts at:      0     801     999 
   Size:        801     198
 
SmlI C'TyrA_G

Cuts at:      0     364     477     999 
   Size:        364     113     522

  Fragments arranged by size: 

                 522     364     113
 
SspI AAT'ATT

Cuts at:      0     390     999 
   Size:        390     609
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     126     999 
   Size:        126     873
 
TatI w'GTAC_w

Cuts at:      0      63     999 
   Size:         63     936
 

 Enzymes that do cut:

     AccI     AleI     ApoI    BamHI     BanI    BanII     BbsI     BcgI
     BglI    BglII Bme1580I    BpuEI     BsaI    BseRI    BseYI Bsp1286I
    BsrBI    BsrFI    BstXI    BstYI   Bsu36I     EaeI EcoO109I     MslI
   MspA1I     NdeI    PvuII     SfcI     SmlI     SspI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI