(Linear) (Six-base) MAPSORT of: KIBB7144.seq  Check: 8076  from: 1  to: 1017



KIBB7144.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     399     911    1017 
   Size:        355      44     512     106

  Fragments arranged by size: 

                 512     355     106      44
 
AflIII A'CryG_T

Cuts at:      0     347    1017 
   Size:        347     670
 
AleI CACnn'nnGTG

Cuts at:      0      85    1017 
   Size:         85     932
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     587     903    1017 
   Size:        587     316     114

  Fragments arranged by size: 

                 587     316     114
 
AlwNI CAG_nnn'CTG

Cuts at:      0     428     604    1017 
   Size:        428     176     413

  Fragments arranged by size: 

                 428     413     176
 
ApoI r'AATT_y

Cuts at:      0      91    1017 
   Size:         91     926
 
BamHI G'GATC_C

Cuts at:      0     900    1017 
   Size:        900     117
 
BanII G_rGCy'C

Cuts at:      0     312    1017 
   Size:        312     705
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     136     376     744    1017 
   Size:        136     240     368     273

  Fragments arranged by size: 

                 368     273     240     136
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     550    1017 
   Size:        550     467
 
BclI T'GATC_A

Cuts at:      0     218     632    1017 
   Size:        218     414     385

  Fragments arranged by size: 

                 414     385     218
 
BlpI GC'TnA_GC

Cuts at:      0     605    1017 
   Size:        605     412
 
BmgBI CAC'GTC

Cuts at:      0     374    1017 
   Size:        374     643
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     622    1017 
   Size:        622     395
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     693    1017 
   Size:        391     302     324

  Fragments arranged by size: 

                 391     324     302
 
BsaBI GATnn'nnATC

Cuts at:      0     637    1017 
   Size:        637     380
 
BsaWI w'CCGG_w

Cuts at:      0     266    1017 
   Size:        266     751
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     394    1017 
   Size:        394     623
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179    1017 
   Size:        179     838
 
BsmI GAATG_Cn'

Cuts at:      0     576    1017 
   Size:        576     441
 
Bsp1286I G_dGCh'C

Cuts at:      0     312    1017 
   Size:        312     705
 
BspEI T'CCGG_A

Cuts at:      0     266    1017 
   Size:        266     751
 
BspHI T'CATG_A

Cuts at:      0     215     983    1017 
   Size:        215     768      34

  Fragments arranged by size: 

                 768     215      34
 
BsrBI CCG'CTC

Cuts at:      0     974    1017 
   Size:        974      43
 
BstYI r'GATC_y

Cuts at:      0     900    1017 
   Size:        900     117
 
BtgI C'CryG_G

Cuts at:      0     800     954    1017 
   Size:        800     154      63

  Fragments arranged by size: 

                 800     154      63
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     624    1017 
   Size:        624     393
 
BtsI GCAGTG_nn'

Cuts at:      0      56     721    1017 
   Size:         56     665     296

  Fragments arranged by size: 

                 665     296      56
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     847     882     978    1013    1017 
   Size:        847      35      96      35       4

  Fragments arranged by size: 

                 847      96      35      35       4
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     399     911    1017 
   Size:        355      44     512     106

  Fragments arranged by size: 

                 512     355     106      44
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     558    1017 
   Size:        558     459
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     608     640     931     963    1017 
   Size:        608      32     291      32      54

  Fragments arranged by size: 

                 608     291      54      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      85     214    1017 
   Size:         85     129     803

  Fragments arranged by size: 

                 803     129      85
 
NcoI C'CATG_G

Cuts at:      0     800     954    1017 
   Size:        800     154      63

  Fragments arranged by size: 

                 800     154      63
 
NspI r_CATG'y

Cuts at:      0     351    1017 
   Size:        351     666
 
PciI A'CATG_T

Cuts at:      0     347    1017 
   Size:        347     670
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     428    1017 
   Size:        428     589
 
PstI C_TGCA'G

Cuts at:      0     750    1017 
   Size:        750     267
 
SfcI C'TryA_G

Cuts at:      0     746    1017 
   Size:        746     271
 
SmlI C'TyrA_G

Cuts at:      0     406     672    1017 
   Size:        406     266     345

  Fragments arranged by size: 

                 406     345     266
 
StyI C'CwwG_G

Cuts at:      0     696     800     954    1017 
   Size:        696     104     154      63

  Fragments arranged by size: 

                 696     154     104      63
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     839     871    1017 
   Size:        839      32     146

  Fragments arranged by size: 

                 839     146      32
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI    BamHI    BanII
     BbsI    BciVI     BclI     BlpI    BmgBI     BmrI    BpuEI    BsaBI
    BsaWI    BseRI     BsgI     BsmI Bsp1286I    BspEI    BspHI    BsrBI
    BstYI     BtgI    BtgZI     BtsI    CspCI  Eco57MI     FalI    Hin4I
     MslI     NcoI     NspI     PciI    PflMI     PstI     SfcI     SmlI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BfrBI     BglI
    BglII Bme1580I     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI