(Linear) (Six-base) MAPSORT of: KIBB7145.seq  Check: 2542  from: 1  to: 1032



KIBB7145.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     356     466    1032 
   Size:        356     110     566

  Fragments arranged by size: 

                 566     356     110
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     924     971    1032 
   Size:        198     726      47      61

  Fragments arranged by size: 

                 726     198      61      47
 
AflIII A'CryG_T

Cuts at:      0     296    1032 
   Size:        296     736
 
AleI CACnn'nnGTG

Cuts at:      0     415    1032 
   Size:        415     617
 
ApaLI G'TGCA_C

Cuts at:      0     443    1032 
   Size:        443     589
 
ApoI r'AATT_y

Cuts at:      0     510     897     969    1032 
   Size:        510     387      72      63

  Fragments arranged by size: 

                 510     387      72      63
 
BclI T'GATC_A

Cuts at:      0     704    1032 
   Size:        704     328
 
Bme1580I G_kGCm'C

Cuts at:      0     447    1032 
   Size:        447     585
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     528    1032 
   Size:        528     504
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     983    1032 
   Size:        983      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     447    1032 
   Size:        447     585
 
BsmI GAATG_Cn'

Cuts at:      0     308    1032 
   Size:        308     724
 
Bsp1286I G_dGCh'C

Cuts at:      0     447    1032 
   Size:        447     585
 
BsrDI GCAATG_nn'

Cuts at:      0     185    1032 
   Size:        185     847
 
BsrGI T'GTAC_A

Cuts at:      0     696    1032 
   Size:        696     336
 
BtsI GCAGTG_nn'

Cuts at:      0      21    1032 
   Size:         21    1011
 
ClaI AT'CG_AT

Cuts at:      0     339    1032 
   Size:        339     693
 
DraI TTT'AAA

Cuts at:      0     596     683    1032 
   Size:        596      87     349

  Fragments arranged by size: 

                 596     349      87
 
EaeI y'GGCC_r

Cuts at:      0     244    1032 
   Size:        244     788
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     924     971    1032 
   Size:        198     726      47      61

  Fragments arranged by size: 

                 726     198      61      47
 
EcoRI G'AATT_C

Cuts at:      0     897    1032 
   Size:        897     135
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1006    1032 
   Size:       1006      26
 
HincII GTy'rAC

Cuts at:      0      12     425    1032 
   Size:         12     413     607

  Fragments arranged by size: 

                 607     413      12
 
HpaI GTT'AAC

Cuts at:      0      12     425    1032 
   Size:         12     413     607

  Fragments arranged by size: 

                 607     413      12
 
MfeI C'AATT_G

Cuts at:      0     102     350    1032 
   Size:        102     248     682

  Fragments arranged by size: 

                 682     248     102
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     869    1032 
   Size:        869     163
 
MscI TGG'CCA

Cuts at:      0     246    1032 
   Size:        246     786
 
MslI CAynn'nnrTG

Cuts at:      0     415    1032 
   Size:        415     617
 
NspI r_CATG'y

Cuts at:      0      69     300    1032 
   Size:         69     231     732

  Fragments arranged by size: 

                 732     231      69
 
PciI A'CATG_T

Cuts at:      0     296    1032 
   Size:        296     736
 
SfcI C'TryA_G

Cuts at:      0     863     988    1032 
   Size:        863     125      44

  Fragments arranged by size: 

                 863     125      44
 
SmlI C'TyrA_G

Cuts at:      0     543    1032 
   Size:        543     489
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      50    1032 
   Size:         50     982
 
TatI w'GTAC_w

Cuts at:      0      47     696    1032 
   Size:         47     649     336

  Fragments arranged by size: 

                 649     336      47
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     524    1032 
   Size:        524     508
 
XmnI GAAnn'nnTTC

Cuts at:      0     512    1032 
   Size:        512     520
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    ApaLI     ApoI     BclI Bme1580I
    BpuEI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrGI     BtsI
     ClaI     DraI     EaeI  Eco57MI    EcoRI    Hin4I   HincII     HpaI
     MfeI     MmeI     MscI     MslI     NspI     PciI     SfcI     SmlI
    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     KasI
     KpnI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI