Restriction map for
pF1KB7151
(Linear) (Six-base) MAPSORT of: KIBB7151.seq Check: 3542 from: 1 to: 1050
KIBB7151.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 54 1050
Size: 54 996
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1050
Size: 612 438
AlwNI CAG_nnn'CTG
Cuts at: 0 502 1050
Size: 502 548
ApaI G_GGCC'C
Cuts at: 0 446 969 1050
Size: 446 523 81
Fragments arranged by size:
523 446 81
ApoI r'AATT_y
Cuts at: 0 9 295 1050
Size: 9 286 755
Fragments arranged by size:
755 286 9
AvaI C'yCGr_G
Cuts at: 0 115 1050
Size: 115 935
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 482 515 1050
Size: 482 33 535
Fragments arranged by size:
535 482 33
BanII G_rGCy'C
Cuts at: 0 446 969 1050
Size: 446 523 81
Fragments arranged by size:
523 446 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 330 897 1050
Size: 330 567 153
Fragments arranged by size:
567 330 153
BclI T'GATC_A
Cuts at: 0 434 1050
Size: 434 616
Bme1580I G_kGCm'C
Cuts at: 0 446 864 969 1050
Size: 446 418 105 81
Fragments arranged by size:
446 418 105 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 979 1050
Size: 56 923 71
Fragments arranged by size:
923 71 56
BsaWI w'CCGG_w
Cuts at: 0 158 611 1050
Size: 158 453 439
Fragments arranged by size:
453 439 158
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 454 1050
Size: 454 596
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 1050
Size: 510 540
Bsp1286I G_dGCh'C
Cuts at: 0 446 864 969 1050
Size: 446 418 105 81
Fragments arranged by size:
446 418 105 81
BspEI T'CCGG_A
Cuts at: 0 158 1050
Size: 158 892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 1050
Size: 54 996
BsrDI GCAATG_nn'
Cuts at: 0 353 469 1050
Size: 353 116 581
Fragments arranged by size:
581 353 116
BsrGI T'GTAC_A
Cuts at: 0 824 1050
Size: 824 226
BstYI r'GATC_y
Cuts at: 0 680 1050
Size: 680 370
BtgI C'CryG_G
Cuts at: 0 203 257 1050
Size: 203 54 793
Fragments arranged by size:
793 203 54
BtsI GCAGTG_nn'
Cuts at: 0 851 1050
Size: 851 199
EaeI y'GGCC_r
Cuts at: 0 200 422 578 1050
Size: 200 222 156 472
Fragments arranged by size:
472 222 200 156
EarI CTCTTCn'nnn_
Cuts at: 0 73 1050
Size: 73 977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 612 979 1050
Size: 56 556 367 71
Fragments arranged by size:
556 367 71 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 973 1050
Size: 973 77
EcoO109I rG'GnC_Cy
Cuts at: 0 120 443 723 965 966 1050
Size: 120 323 280 242 1 84
Fragments arranged by size:
323 280 242 120 84 1
EcoRV GAT'ATC
Cuts at: 0 78 1050
Size: 78 972
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 711 1050
Size: 711 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 593 625 1050
Size: 593 32 425
Fragments arranged by size:
593 425 32
HincII GTy'rAC
Cuts at: 0 593 1050
Size: 593 457
MscI TGG'CCA
Cuts at: 0 424 1050
Size: 424 626
MslI CAynn'nnrTG
Cuts at: 0 487 1050
Size: 487 563
MspA1I CmG'CkG
Cuts at: 0 368 881 1050
Size: 368 513 169
Fragments arranged by size:
513 368 169
NcoI C'CATG_G
Cuts at: 0 257 1050
Size: 257 793
NspI r_CATG'y
Cuts at: 0 825 1050
Size: 825 225
PasI CC'CwG_GG
Cuts at: 0 446 1050
Size: 446 604
PfoI T'CCnGG_A
Cuts at: 0 71 923 1050
Size: 71 852 127
Fragments arranged by size:
852 127 71
PpuMI rG'GwC_Cy
Cuts at: 0 120 723 1050
Size: 120 603 327
Fragments arranged by size:
603 327 120
PspOMI G'GGCC_C
Cuts at: 0 442 965 1050
Size: 442 523 85
Fragments arranged by size:
523 442 85
PstI C_TGCA'G
Cuts at: 0 1029 1050
Size: 1029 21
PvuII CAG'CTG
Cuts at: 0 881 1050
Size: 881 169
SexAI A'CCwGG_T
Cuts at: 0 501 1050
Size: 501 549
SfcI C'TryA_G
Cuts at: 0 494 1025 1050
Size: 494 531 25
Fragments arranged by size:
531 494 25
StyI C'CwwG_G
Cuts at: 0 257 1050
Size: 257 793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 438 651 988 1050
Size: 135 303 213 337 62
Fragments arranged by size:
337 303 213 135 62
TatI w'GTAC_w
Cuts at: 0 824 1050
Size: 824 226
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BaeI BanII
BbsI BclI Bme1580I BpmI BsaWI BseRI BsgI Bsp1286I
BspEI BspMI BsrDI BsrGI BstYI BtgI BtsI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII
MscI MslI MspA1I NcoI NspI PasI PfoI PpuMI
PspOMI PstI PvuII SexAI SfcI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI