(Linear) (Six-base) MAPSORT of: KIBB7156.seq Check: 8870 from: 1 to: 1077 KIBB7156.orf With 182 enzymes: SgfI * March 3, 2008 10:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 451 1077 Size: 451 626 AccI GT'mk_AC Cuts at: 0 257 1077 Size: 257 820 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 196 296 1077 Size: 41 155 100 781 Fragments arranged by size: 781 155 100 41 AflIII A'CryG_T Cuts at: 0 358 1077 Size: 358 719 ApoI r'AATT_y Cuts at: 0 204 561 680 922 1077 Size: 204 357 119 242 155 Fragments arranged by size: 357 242 204 155 119 BbsI GAAGACnn'nnnn_ Cuts at: 0 184 1077 Size: 184 893 BclI T'GATC_A Cuts at: 0 626 1077 Size: 626 451 BmrI ACTGGGnnnn_n' Cuts at: 0 613 1077 Size: 613 464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 925 1077 Size: 925 152 BsaAI yAC'GTr Cuts at: 0 359 814 1077 Size: 359 455 263 Fragments arranged by size: 455 359 263 BseYI C'CCAG_C Cuts at: 0 582 1077 Size: 582 495 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1040 1077 Size: 1040 37 BsiHKAI G_wGCw'C Cuts at: 0 1006 1077 Size: 1006 71 Bsp1286I G_dGCh'C Cuts at: 0 1006 1077 Size: 1006 71 BspHI T'CATG_A Cuts at: 0 86 1077 Size: 86 991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 451 1077 Size: 451 626 ClaI AT'CG_AT Cuts at: 0 375 1077 Size: 375 702 DraI TTT'AAA Cuts at: 0 927 1077 Size: 927 150 EaeI y'GGCC_r Cuts at: 0 185 473 845 1077 Size: 185 288 372 232 Fragments arranged by size: 372 288 232 185 EarI CTCTTCn'nnn_ Cuts at: 0 18 1077 Size: 18 1059 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 41 196 296 925 1077 Size: 41 155 100 629 152 Fragments arranged by size: 629 155 152 100 41 EcoNI CCTnn'n_nnAGG Cuts at: 0 667 1077 Size: 667 410 EcoO109I rG'GnC_Cy Cuts at: 0 660 1077 Size: 660 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 923 955 1077 Size: 923 32 122 Fragments arranged by size: 923 122 32 MfeI C'AATT_G Cuts at: 0 731 882 1077 Size: 731 151 195 Fragments arranged by size: 731 195 151 MscI TGG'CCA Cuts at: 0 187 475 1077 Size: 187 288 602 Fragments arranged by size: 602 288 187 MslI CAynn'nnrTG Cuts at: 0 115 265 500 847 1077 Size: 115 150 235 347 230 Fragments arranged by size: 347 235 230 150 115 MspA1I CmG'CkG Cuts at: 0 278 1077 Size: 278 799 PfoI T'CCnGG_A Cuts at: 0 754 1077 Size: 754 323 PmlI CAC'GTG Cuts at: 0 359 1077 Size: 359 718 SfcI C'TryA_G Cuts at: 0 819 1077 Size: 819 258 SspI AAT'ATT Cuts at: 0 128 528 773 1077 Size: 128 400 245 304 Fragments arranged by size: 400 304 245 128 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 410 1077 Size: 410 667 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 600 1077 Size: 600 477 Enzymes that do cut: AarI AccI AcuI AflIII ApoI BbsI BclI BmrI BpmI BsaAI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI ClaI DraI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MfeI MscI MslI MspA1I PfoI PmlI SfcI SspI TaqII XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI