(Linear) (Six-base) MAPSORT of: KIBB7158.seq  Check: 121  from: 1  to: 1089



KIBB7158.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     415    1089 
   Size:        415     674
 
AatII G_ACGT'C

Cuts at:      0     551    1089 
   Size:        551     538
 
AccI GT'mk_AC

Cuts at:      0      91    1089 
   Size:         91     998
 
Acc65I G'GTAC_C

Cuts at:      0     425    1089 
   Size:        425     664
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736     784    1089 
   Size:        736      48     305

  Fragments arranged by size: 

                 736     305      48
 
AflIII A'CryG_T

Cuts at:      0     380    1089 
   Size:        380     709
 
AgeI A'CCGG_T

Cuts at:      0     968    1089 
   Size:        968     121
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     149    1089 
   Size:        149     940
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1041    1073    1089 
   Size:       1041      32      16

  Fragments arranged by size: 

                1041      32      16
 
AlwNI CAG_nnn'CTG

Cuts at:      0     778    1089 
   Size:        778     311
 
AseI AT'TA_AT

Cuts at:      0     860    1089 
   Size:        860     229
 
BanI G'GyrC_C

Cuts at:      0     425    1089 
   Size:        425     664
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      43     724    1089 
   Size:         43     681     365

  Fragments arranged by size: 

                 681     365      43
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1069    1089 
   Size:       1069      20
 
BsaHI Gr'CG_yC

Cuts at:      0     548    1089 
   Size:        548     541
 
BsaWI w'CCGG_w

Cuts at:      0     968    1089 
   Size:        968     121
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     968     998    1089 
   Size:        968      30      91

  Fragments arranged by size: 

                 968      91      30
 
BseYI C'CCAG_C

Cuts at:      0     625    1089 
   Size:        625     464
 
BsmI GAATG_Cn'

Cuts at:      0     582    1089 
   Size:        582     507
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     539    1089 
   Size:        539     550
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     415    1089 
   Size:        415     674
 
BsrDI GCAATG_nn'

Cuts at:      0     523    1089 
   Size:        523     566
 
BsrFI r'CCGG_y

Cuts at:      0     968    1089 
   Size:        968     121
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     915    1013    1089 
   Size:        915      98      76

  Fragments arranged by size: 

                 915      98      76
 
BtsI GCAGTG_nn'

Cuts at:      0     996    1089 
   Size:        996      93
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1083    1089 
   Size:       1083       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     293    1089 
   Size:        293     796
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     736     784    1089 
   Size:        736      48     305

  Fragments arranged by size: 

                 736     305      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     220    1089 
   Size:        220     869
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     164     196    1024    1056    1089 
   Size:        164      32     828      32      33

  Fragments arranged by size: 

                 828     164      33      32      32
 
KpnI G_GTAC'C

Cuts at:      0     429    1089 
   Size:        429     660
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     942     999    1089 
   Size:        942      57      90

  Fragments arranged by size: 

                 942      90      57
 
MslI CAynn'nnrTG

Cuts at:      0     131     409    1089 
   Size:        131     278     680

  Fragments arranged by size: 

                 680     278     131
 
MspA1I CmG'CkG

Cuts at:      0      47     625     775     932    1089 
   Size:         47     578     150     157     157

  Fragments arranged by size: 

                 578     157     157     150      47
 
NspI r_CATG'y

Cuts at:      0     384     689    1089 
   Size:        384     305     400

  Fragments arranged by size: 

                 400     384     305
 
PciI A'CATG_T

Cuts at:      0     380    1089 
   Size:        380     709
 
PfoI T'CCnGG_A

Cuts at:      0     830    1089 
   Size:        830     259
 
PpuMI rG'GwC_Cy

Cuts at:      0     220    1089 
   Size:        220     869
 
PvuII CAG'CTG

Cuts at:      0     625     775     932    1089 
   Size:        625     150     157     157

  Fragments arranged by size: 

                 625     157     157     150
 
SspI AAT'ATT

Cuts at:      0     186     674    1089 
   Size:        186     488     415

  Fragments arranged by size: 

                 488     415     186
 
StyI C'CwwG_G

Cuts at:      0     651    1020    1089 
   Size:        651     369      69

  Fragments arranged by size: 

                 651     369      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     841    1089 
   Size:        841     248
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     735     767    1089 
   Size:        735      32     322

  Fragments arranged by size: 

                 735     322      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     757    1089 
   Size:        757     332
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     622    1089 
   Size:        622     467
 
ZraI GAC'GTC

Cuts at:      0     549    1089 
   Size:        549     540
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AgeI     AlfI
     AloI    AlwNI     AseI     BanI     BbsI     BsaI    BsaHI    BsaWI
    BsaXI    BseYI     BsmI    BsmBI    BspMI    BsrDI    BsrFI    BstXI
     BtsI     DrdI     EarI  Eco57MI EcoO109I    Hin4I     KpnI     MmeI
     MslI   MspA1I     NspI     PciI     PfoI    PpuMI    PvuII     SspI
     StyI    TaqII     TstI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AhdI     AleI     ApaI    ApaLI     ApoI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TatI     XbaI     XhoI     XmaI     XmnI