(Linear) (Six-base) MAPSORT of: KIBB7159.seq  Check: 1615  from: 1  to: 1095



KIBB7159.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     328    1095 
   Size:        328     767
 
AflII C'TTAA_G

Cuts at:      0     663    1095 
   Size:        663     432
 
AleI CACnn'nnGTG

Cuts at:      0     338    1095 
   Size:        338     757
 
AlwNI CAG_nnn'CTG

Cuts at:      0     362    1095 
   Size:        362     733
 
ApaLI G'TGCA_C

Cuts at:      0     516    1095 
   Size:        516     579
 
ApoI r'AATT_y

Cuts at:      0     690    1005    1095 
   Size:        690     315      90

  Fragments arranged by size: 

                 690     315      90
 
BamHI G'GATC_C

Cuts at:      0     791    1095 
   Size:        791     304
 
BanI G'GyrC_C

Cuts at:      0     142    1095 
   Size:        142     953
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1052    1095 
   Size:       1052      43
 
BclI T'GATC_A

Cuts at:      0     128    1013    1095 
   Size:        128     885      82

  Fragments arranged by size: 

                 885     128      82
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34     412    1095 
   Size:         34     378     683

  Fragments arranged by size: 

                 683     378      34
 
BglII A'GATC_T

Cuts at:      0     839    1095 
   Size:        839     256
 
Bme1580I G_kGCm'C

Cuts at:      0     520    1095 
   Size:        520     575
 
BmgBI CAC'GTC

Cuts at:      0     989    1095 
   Size:        989     106
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     372    1095 
   Size:        372     723
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     498    1095 
   Size:        498     597
 
Bpu10I CC'TnA_GC

Cuts at:      0     232     496    1095 
   Size:        232     264     599

  Fragments arranged by size: 

                 599     264     232
 
BsaBI GATnn'nnATC

Cuts at:      0     815    1095 
   Size:        815     280
 
BsaWI w'CCGG_w

Cuts at:      0     608    1095 
   Size:        608     487
 
BseYI C'CCAG_C

Cuts at:      0     561    1095 
   Size:        561     534
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300    1095 
   Size:        300     795
 
BsiHKAI G_wGCw'C

Cuts at:      0     221     520    1095 
   Size:        221     299     575

  Fragments arranged by size: 

                 575     299     221
 
BsmI GAATG_Cn'

Cuts at:      0     895    1095 
   Size:        895     200
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     520    1095 
   Size:        221     299     575

  Fragments arranged by size: 

                 575     299     221
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     319     328    1095 
   Size:        319       9     767

  Fragments arranged by size: 

                 767     319       9
 
BsrDI GCAATG_nn'

Cuts at:      0     344    1095 
   Size:        344     751
 
BstYI r'GATC_y

Cuts at:      0     791     839    1095 
   Size:        791      48     256

  Fragments arranged by size: 

                 791     256      48
 
BtsI GCAGTG_nn'

Cuts at:      0     221    1095 
   Size:        221     874
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     747     782    1095 
   Size:        747      35     313

  Fragments arranged by size: 

                 747     313      35
 
DraI TTT'AAA

Cuts at:      0    1065    1095 
   Size:       1065      30
 
EaeI y'GGCC_r

Cuts at:      0     191     404     623     974    1095 
   Size:        191     213     219     351     121

  Fragments arranged by size: 

                 351     219     213     191     121
 
EarI CTCTTCn'nnn_

Cuts at:      0     391    1095 
   Size:        391     704
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     498    1095 
   Size:        498     597
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     283     786    1095 
   Size:        283     503     309

  Fragments arranged by size: 

                 503     309     283
 
EcoRI G'AATT_C

Cuts at:      0     690    1005    1095 
   Size:        690     315      90

  Fragments arranged by size: 

                 690     315      90
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     927     959    1005    1037    1095 
   Size:        927      32      46      32      58

  Fragments arranged by size: 

                 927      58      46      32      32
 
HindIII A'AGCT_T

Cuts at:      0     265    1095 
   Size:        265     830
 
MslI CAynn'nnrTG

Cuts at:      0      76     250     338     460     533    1095 
   Size:         76     174      88     122      73     562

  Fragments arranged by size: 

                 562     174     122      88      76      73
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     412    1095 
   Size:        412     683
 
SmlI C'TyrA_G

Cuts at:      0     663    1095 
   Size:        663     432
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     553     836     880    1095 
   Size:        553     283      44     215

  Fragments arranged by size: 

                 553     283     215      44
 
XbaI T'CTAG_A

Cuts at:      0     813    1095 
   Size:        813     282
 

 Enzymes that do cut:

     AarI    AflII     AleI    AlwNI    ApaLI     ApoI    BamHI     BanI
     BbsI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BsaBI    BsaWI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BstYI     BtsI    CspCI     DraI     EaeI     EarI
  Eco57MI    EcoNI    EcoRI    Hin4I  HindIII     MslI     SfiI     SmlI
    TaqII     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BlpI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI   DraIII     DrdI     EagI     EciI
  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI