(Linear) (Six-base) MAPSORT of: KIBB7166.seq  Check: 2243  from: 1  to: 1188



KIBB7166.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AleI CACnn'nnGTG

Cuts at:      0      55    1188 
   Size:         55    1133
 
AlwNI CAG_nnn'CTG

Cuts at:      0     543    1188 
   Size:        543     645
 
ApoI r'AATT_y

Cuts at:      0     148    1188 
   Size:        148    1040
 
AvaI C'yCGr_G

Cuts at:      0    1094    1188 
   Size:       1094      94
 
BamHI G'GATC_C

Cuts at:      0     408     810    1188 
   Size:        408     402     378

  Fragments arranged by size: 

                 408     402     378
 
BanII G_rGCy'C

Cuts at:      0     378     518    1188 
   Size:        378     140     670

  Fragments arranged by size: 

                 670     378     140
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      81    1177    1188 
   Size:         81    1096      11

  Fragments arranged by size: 

                1096      81      11
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     431    1188 
   Size:        431     757
 
BglII A'GATC_T

Cuts at:      0      65    1188 
   Size:         65    1123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1024    1188 
   Size:       1024     164
 
Bpu10I CC'TnA_GC

Cuts at:      0     479    1034    1188 
   Size:        479     555     154

  Fragments arranged by size: 

                 555     479     154
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     826    1188 
   Size:        826     362
 
BsaWI w'CCGG_w

Cuts at:      0      92    1188 
   Size:         92    1096
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     131     406     997    1188 
   Size:         46      85     275     591     191

  Fragments arranged by size: 

                 591     275     191      85      46
 
BseYI C'CCAG_C

Cuts at:      0     993    1126    1188 
   Size:        993     133      62

  Fragments arranged by size: 

                 993     133      62
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     645    1188 
   Size:        645     543
 
BsiHKAI G_wGCw'C

Cuts at:      0     311    1188 
   Size:        311     877
 
Bsp1286I G_dGCh'C

Cuts at:      0     311     378     518    1188 
   Size:        311      67     140     670

  Fragments arranged by size: 

                 670     311     140      67
 
BspEI T'CCGG_A

Cuts at:      0      92    1188 
   Size:         92    1096
 
BspHI T'CATG_A

Cuts at:      0     518    1188 
   Size:        518     670
 
BsrDI GCAATG_nn'

Cuts at:      0     359     872    1188 
   Size:        359     513     316

  Fragments arranged by size: 

                 513     359     316
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57    1188 
   Size:         57    1131
 
BstYI r'GATC_y

Cuts at:      0      65     408     810    1047    1188 
   Size:         65     343     402     237     141

  Fragments arranged by size: 

                 402     343     237     141      65
 
BtsI GCAGTG_nn'

Cuts at:      0    1142    1188 
   Size:       1142      46
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     638    1188 
   Size:        638     550
 
EaeI y'GGCC_r

Cuts at:      0     489    1188 
   Size:        489     699
 
EarI CTCTTCn'nnn_

Cuts at:      0     398    1188 
   Size:        398     790
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1024    1188 
   Size:       1024     164
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      28    1188 
   Size:         28    1160
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     705    1188 
   Size:        705     483
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     110     142    1188 
   Size:        110      32    1046

  Fragments arranged by size: 

                1046     110      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1157    1188 
   Size:       1157      31
 
MslI CAynn'nnrTG

Cuts at:      0      55     247    1188 
   Size:         55     192     941

  Fragments arranged by size: 

                 941     192      55
 
MspA1I CmG'CkG

Cuts at:      0     109     997    1017    1188 
   Size:        109     888      20     171

  Fragments arranged by size: 

                 888     171     109      20
 
PshAI GACnn'nnGTC

Cuts at:      0    1177    1188 
   Size:       1177      11
 
PsiI TTA'TAA

Cuts at:      0     824    1188 
   Size:        824     364
 
PstI C_TGCA'G

Cuts at:      0     539    1016    1188 
   Size:        539     477     172

  Fragments arranged by size: 

                 539     477     172
 
PvuII CAG'CTG

Cuts at:      0     109     997    1188 
   Size:        109     888     191

  Fragments arranged by size: 

                 888     191     109
 
SfcI C'TryA_G

Cuts at:      0     110     535    1012    1065    1188 
   Size:        110     425     477      53     123

  Fragments arranged by size: 

                 477     425     123     110      53
 
SmaI CCC'GGG

Cuts at:      0    1096    1188 
   Size:       1096      92
 
SmlI C'TyrA_G

Cuts at:      0     805    1188 
   Size:        805     383
 
StuI AGG'CCT

Cuts at:      0     719    1063    1188 
   Size:        719     344     125

  Fragments arranged by size: 

                 719     344     125
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     219    1188 
   Size:        219     969
 
TatI w'GTAC_w

Cuts at:      0     899    1188 
   Size:        899     289
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      31      63    1188 
   Size:         31      32    1125

  Fragments arranged by size: 

                1125      32      31
 
XbaI T'CTAG_A

Cuts at:      0     667    1188 
   Size:        667     521
 
XmaI C'CCGG_G

Cuts at:      0    1094    1188 
   Size:       1094      94
 

 Enzymes that do cut:

     AleI    AlwNI     ApoI     AvaI    BamHI    BanII     BbsI    BciVI
    BglII     BpmI   Bpu10I    BpuEI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspEI    BspHI    BsrDI    BstXI    BstYI     BtsI
     DrdI     EaeI     EarI  Eco57MI    EcoNI     FalI    Hin4I     MmeI
     MslI   MspA1I    PshAI     PsiI     PstI    PvuII     SfcI     SmaI
     SmlI     StuI    TaqII     TatI     TstI     XbaI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
  Tth111I     XcmI     XhoI     XmnI     ZraI