Restriction map for
pF1KB7170
(Linear) (Six-base) MAPSORT of: KIBB7170.seq Check: 3614 from: 1 to: 1317
KIBB7170.orf
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 389 1317
Size: 389 928
AleI CACnn'nnGTG
Cuts at: 0 64 1317
Size: 64 1253
AlwNI CAG_nnn'CTG
Cuts at: 0 696 1047 1071 1090 1317
Size: 696 351 24 19 227
Fragments arranged by size:
696 351 227 24 19
ApaI G_GGCC'C
Cuts at: 0 580 1151 1317
Size: 580 571 166
Fragments arranged by size:
580 571 166
ApaLI G'TGCA_C
Cuts at: 0 295 531 1317
Size: 295 236 786
Fragments arranged by size:
786 295 236
AvaI C'yCGr_G
Cuts at: 0 351 371 573 1028 1142 1317
Size: 351 20 202 455 114 175
Fragments arranged by size:
455 351 202 175 114 20
BanI G'GyrC_C
Cuts at: 0 329 490 1162 1228 1317
Size: 329 161 672 66 89
Fragments arranged by size:
672 329 161 89 66
BanII G_rGCy'C
Cuts at: 0 413 437 580 1151 1317
Size: 413 24 143 571 166
Fragments arranged by size:
571 413 166 143 24
BbeI G_GCGC'C
Cuts at: 0 333 494 1166 1317
Size: 333 161 672 151
Fragments arranged by size:
672 333 161 151
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 234 789 1306 1317
Size: 90 144 555 517 11
Fragments arranged by size:
555 517 144 90 11
BglI GCCn_nnn'nGGC
Cuts at: 0 905 1066 1226 1317
Size: 905 161 160 91
Fragments arranged by size:
905 161 160 91
Bme1580I G_kGCm'C
Cuts at: 0 299 535 580 1151 1204 1317
Size: 299 236 45 571 53 113
Fragments arranged by size:
571 299 236 113 53 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 58 1317
Size: 36 22 1259
Fragments arranged by size:
1259 36 22
Bpu10I CC'TnA_GC
Cuts at: 0 407 1317
Size: 407 910
BsaAI yAC'GTr
Cuts at: 0 228 639 756 816 1317
Size: 228 411 117 60 501
Fragments arranged by size:
501 411 228 117 60
BsaHI Gr'CG_yC
Cuts at: 0 206 330 491 1163 1317
Size: 206 124 161 672 154
Fragments arranged by size:
672 206 161 154 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 514 544 1317
Size: 40 30 444 30 773
Fragments arranged by size:
773 444 40 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 442 445 469 472 475 478 481
Size: 55 387 3 24 3 3 3 3
Cuts at: 481 484 523 538 1317
Size: 3 39 15 779
Fragments arranged by size:
779 387 55 39 24 15 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 441 644 1090 1317
Size: 441 203 446 227
Fragments arranged by size:
446 441 227 203
BsiEI CG_ry'CG
Cuts at: 0 545 584 721 900 971 1019 1104 1317
Size: 545 39 137 179 71 48 85 213
Fragments arranged by size:
545 213 179 137 85 71 48 39
BsiHKAI G_wGCw'C
Cuts at: 0 299 535 1317
Size: 299 236 782
Fragments arranged by size:
782 299 236
BsmBI CGTCTCn'nnnn_
Cuts at: 0 466 1317
Size: 466 851
Bsp1286I G_dGCh'C
Cuts at: 0 299 413 437 535 580 1151 1204 1317
Size: 299 114 24 98 45 571 53 113
Fragments arranged by size:
571 299 114 113 98 53 45 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 389 704 1317
Size: 389 315 613
Fragments arranged by size:
613 389 315
BsrBI CCG'CTC
Cuts at: 0 327 1239 1317
Size: 327 912 78
Fragments arranged by size:
912 327 78
BsrFI r'CCGG_y
Cuts at: 0 397 493 1220 1317
Size: 397 96 727 97
Fragments arranged by size:
727 397 97 96
BssHII G'CGCG_C
Cuts at: 0 365 878 1023 1317
Size: 365 513 145 294
Fragments arranged by size:
513 365 294 145
BssSI C'ACGA_G
Cuts at: 0 1234 1317
Size: 1234 83
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1071 1317
Size: 1071 246
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 1317
Size: 66 1251
BstYI r'GATC_y
Cuts at: 0 44 1317
Size: 44 1273
BtgI C'CryG_G
Cuts at: 0 903 937 1317
Size: 903 34 380
Fragments arranged by size:
903 380 34
BtsI GCAGTG_nn'
Cuts at: 0 1191 1284 1317
Size: 1191 93 33
Fragments arranged by size:
1191 93 33
DraIII CAC_nnn'GTG
Cuts at: 0 759 1310 1317
Size: 759 551 7
Fragments arranged by size:
759 551 7
EaeI y'GGCC_r
Cuts at: 0 653 897 968 1016 1240 1317
Size: 653 244 71 48 224 77
Fragments arranged by size:
653 244 224 77 71 48
EagI C'GGCC_G
Cuts at: 0 897 968 1016 1317
Size: 897 71 48 301
Fragments arranged by size:
897 301 71 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 615 909 1317
Size: 331 284 294 408
Fragments arranged by size:
408 331 294 284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 58 1317
Size: 36 22 1259
Fragments arranged by size:
1259 36 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 451 1317
Size: 424 27 866
Fragments arranged by size:
866 424 27
EcoO109I rG'GnC_Cy
Cuts at: 0 569 621 1147 1148 1317
Size: 569 52 526 1 169
Fragments arranged by size:
569 526 169 52 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 813 1317
Size: 813 504
FseI GG_CCGG'CC
Cuts at: 0 1224 1317
Size: 1224 93
HaeII r_GCGC'y
Cuts at: 0 333 494 551 1072 1166 1317
Size: 333 161 57 521 94 151
Fragments arranged by size:
521 333 161 151 94 57
KasI G'GCGC_C
Cuts at: 0 329 490 1162 1317
Size: 329 161 672 155
Fragments arranged by size:
672 329 161 155
MscI TGG'CCA
Cuts at: 0 655 1317
Size: 655 662
MslI CAynn'nnrTG
Cuts at: 0 64 1317
Size: 64 1253
MspA1I CmG'CkG
Cuts at: 0 615 648 824 905 939 973 1087 1317
Size: 615 33 176 81 34 34 114 230
Fragments arranged by size:
615 230 176 114 81 34 34 33
NaeI GCC'GGC
Cuts at: 0 399 495 1222 1317
Size: 399 96 727 95
Fragments arranged by size:
727 399 96 95
NarI GG'CG_CC
Cuts at: 0 330 491 1163 1317
Size: 330 161 672 154
Fragments arranged by size:
672 330 161 154
NgoMIV G'CCGG_C
Cuts at: 0 397 493 1220 1317
Size: 397 96 727 97
Fragments arranged by size:
727 397 97 96
NotI GC'GGCC_GC
Cuts at: 0 897 968 1317
Size: 897 71 349
Fragments arranged by size:
897 349 71
PasI CC'CwG_GG
Cuts at: 0 1256 1317
Size: 1256 61
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 651 1047 1310 1317
Size: 252 399 396 263 7
Fragments arranged by size:
399 396 263 252 7
PfoI T'CCnGG_A
Cuts at: 0 1174 1317
Size: 1174 143
PmlI CAC'GTG
Cuts at: 0 228 756 1317
Size: 228 528 561
Fragments arranged by size:
561 528 228
PpuMI rG'GwC_Cy
Cuts at: 0 569 1317
Size: 569 748
PspOMI G'GGCC_C
Cuts at: 0 576 1147 1317
Size: 576 571 170
Fragments arranged by size:
576 571 170
PstI C_TGCA'G
Cuts at: 0 736 1067 1317
Size: 736 331 250
Fragments arranged by size:
736 331 250
PvuII CAG'CTG
Cuts at: 0 648 824 1317
Size: 648 176 493
Fragments arranged by size:
648 493 176
SacII CC_GC'GG
Cuts at: 0 906 940 1317
Size: 906 34 377
Fragments arranged by size:
906 377 34
SanDI GG'GwC_CC
Cuts at: 0 569 1317
Size: 569 748
SbfI CC_TGCA'GG
Cuts at: 0 1067 1317
Size: 1067 250
SfcI C'TryA_G
Cuts at: 0 732 996 1063 1317
Size: 732 264 67 254
Fragments arranged by size:
732 264 254 67
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 905 1317
Size: 905 412
SfoI GGC'GCC
Cuts at: 0 331 492 1164 1317
Size: 331 161 672 153
Fragments arranged by size:
672 331 161 153
SgrAI Cr'CCGG_yG
Cuts at: 0 493 1317
Size: 493 824
SmaI CCC'GGG
Cuts at: 0 353 575 1144 1317
Size: 353 222 569 173
Fragments arranged by size:
569 353 222 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 1246 1317
Size: 261 985 71
Fragments arranged by size:
985 261 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 514 546 1170 1202 1317
Size: 40 32 442 32 624 32 115
Fragments arranged by size:
624 442 115 40 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 193 1317
Size: 193 1124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 1317
Size: 444 873
XmaI C'CCGG_G
Cuts at: 0 351 573 1142 1317
Size: 351 222 569 175
Fragments arranged by size:
569 351 222 175
Enzymes that do cut:
AarI AleI AlwNI ApaI ApaLI AvaI BanI BanII
BbeI BbsI BglI Bme1580I BpmI Bpu10I BsaAI BsaHI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI
BtsI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I
FalI FseI HaeII KasI MscI MslI MspA1I NaeI
NarI NgoMIV NotI PasI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacII SanDI SbfI SfcI SfiI
SfoI SgrAI SmaI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EarI EcoICRI EcoRI EcoRV FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NruI NsiI NspI PacI PciI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SapI ScaI SexAI SgfI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
XbaI XhoI XmnI ZraI