Restriction map for
pF1KB7171
(Linear) (Six-base) MAPSORT of: KIBB7171.seq Check: 2168 from: 1 to: 1446
KIBB7171.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1356 1446
Size: 169 1187 90
Fragments arranged by size:
1187 169 90
AfeI AGC'GCT
Cuts at: 0 897 1446
Size: 897 549
AflIII A'CryG_T
Cuts at: 0 314 1387 1446
Size: 314 1073 59
Fragments arranged by size:
1073 314 59
ApoI r'AATT_y
Cuts at: 0 523 1355 1446
Size: 523 832 91
Fragments arranged by size:
832 523 91
AvrII C'CTAG_G
Cuts at: 0 727 1446
Size: 727 719
BanII G_rGCy'C
Cuts at: 0 62 1385 1446
Size: 62 1323 61
Fragments arranged by size:
1323 62 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 321 675 1071 1446
Size: 321 354 396 375
Fragments arranged by size:
396 375 354 321
BclI T'GATC_A
Cuts at: 0 657 1446
Size: 657 789
BfrBI ATG'CAT
Cuts at: 0 1259 1446
Size: 1259 187
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 115 803 1446
Size: 58 57 688 643
Fragments arranged by size:
688 643 58 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 519 632 1381 1446
Size: 235 284 113 749 65
Fragments arranged by size:
749 284 235 113 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 535 590 798 1446
Size: 13 522 55 208 648
Fragments arranged by size:
648 522 208 55 13
BsaBI GATnn'nnATC
Cuts at: 0 977 1446
Size: 977 469
BsaHI Gr'CG_yC
Cuts at: 0 4 1446
Size: 4 1442
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 1393 1446
Size: 196 1197 53
Fragments arranged by size:
1197 196 53
BsiHKAI G_wGCw'C
Cuts at: 0 1385 1446
Size: 1385 61
BsmI GAATG_Cn'
Cuts at: 0 461 1091 1446
Size: 461 630 355
Fragments arranged by size:
630 461 355
Bsp1286I G_dGCh'C
Cuts at: 0 62 1385 1446
Size: 62 1323 61
Fragments arranged by size:
1323 62 61
BspHI T'CATG_A
Cuts at: 0 1052 1446
Size: 1052 394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 789 1446
Size: 263 526 657
Fragments arranged by size:
657 526 263
BsrDI GCAATG_nn'
Cuts at: 0 126 408 1198 1259 1446
Size: 126 282 790 61 187
Fragments arranged by size:
790 282 187 126 61
BssSI C'ACGA_G
Cuts at: 0 997 1446
Size: 997 449
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 846 1186 1446
Size: 351 495 340 260
Fragments arranged by size:
495 351 340 260
BstYI r'GATC_y
Cuts at: 0 567 633 1446
Size: 567 66 813
Fragments arranged by size:
813 567 66
BtgI C'CryG_G
Cuts at: 0 49 77 1136 1446
Size: 49 28 1059 310
Fragments arranged by size:
1059 310 49 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 382 1446
Size: 382 1064
DraI TTT'AAA
Cuts at: 0 777 881 1446
Size: 777 104 565
Fragments arranged by size:
777 565 104
EaeI y'GGCC_r
Cuts at: 0 1133 1446
Size: 1133 313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 214 1446
Size: 214 1232
EcoICRI GAG'CTC
Cuts at: 0 1383 1446
Size: 1383 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 235 519 632 1356 1381 1446
Size: 169 66 284 113 724 25 65
Fragments arranged by size:
724 284 169 113 66 65 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 270 1446
Size: 270 1176
EcoRI G'AATT_C
Cuts at: 0 1355 1446
Size: 1355 91
HaeII r_GCGC'y
Cuts at: 0 899 1446
Size: 899 547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 625 657 778 810 1446
Size: 25 32 568 32 121 32 636
Fragments arranged by size:
636 568 121 32 32 32 25
HincII GTy'rAC
Cuts at: 0 746 1446
Size: 746 700
HpaI GTT'AAC
Cuts at: 0 746 1446
Size: 746 700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 1446
Size: 711 735
MslI CAynn'nnrTG
Cuts at: 0 48 401 466 1446
Size: 48 353 65 980
Fragments arranged by size:
980 353 65 48
MspA1I CmG'CkG
Cuts at: 0 159 1446
Size: 159 1287
NcoI C'CATG_G
Cuts at: 0 49 77 1446
Size: 49 28 1369
Fragments arranged by size:
1369 49 28
NsiI A_TGCA'T
Cuts at: 0 1261 1446
Size: 1261 185
NspI r_CATG'y
Cuts at: 0 318 1446
Size: 318 1128
PciI A'CATG_T
Cuts at: 0 314 1446
Size: 314 1132
PflMI CCAn_nnn'nTGG
Cuts at: 0 121 1446
Size: 121 1325
PfoI T'CCnGG_A
Cuts at: 0 941 1446
Size: 941 505
PstI C_TGCA'G
Cuts at: 0 717 1446
Size: 717 729
SacI G_AGCT'C
Cuts at: 0 1385 1446
Size: 1385 61
SfcI C'TryA_G
Cuts at: 0 494 713 1446
Size: 494 219 733
Fragments arranged by size:
733 494 219
StyI C'CwwG_G
Cuts at: 0 49 77 194 664 727 1223 1446
Size: 49 28 117 470 63 496 223
Fragments arranged by size:
496 470 223 117 63 49 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 126 429 1411 1446
Size: 126 303 982 35
Fragments arranged by size:
982 303 126 35
TatI w'GTAC_w
Cuts at: 0 1435 1446
Size: 1435 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 788 820 923 955 1386 1418 1446
Size: 788 32 103 32 431 32 28
Fragments arranged by size:
788 431 103 32 32 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 511 1446
Size: 511 935
Enzymes that do cut:
AcuI AfeI AflIII ApoI AvrII BanII BbsI BclI
BfrBI BmrI BpmI BsaI BsaBI BsaHI BseRI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BssSI BstXI BstYI
BtgI BtgZI DraI EaeI EciI EcoICRI Eco57MI EcoNI
EcoRI HaeII Hin4I HincII HpaI MmeI MslI MspA1I
NcoI NsiI NspI PciI PflMI PfoI PstI SacI
SfcI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraIII
DrdI EagI EarI EcoO109I EcoRV FalI FseI FspI
FspAI HindIII KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI