Restriction map for
pF1KB7179
(Linear) (Six-base) MAPSORT of: KIBB7179.seq Check: 5953 from: 1 to: 1437
KIBB7179.orf
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 718 1437
Size: 365 353 719
Fragments arranged by size:
719 365 353
AflIII A'CryG_T
Cuts at: 0 1031 1437
Size: 1031 406
AleI CACnn'nnGTG
Cuts at: 0 400 974 1437
Size: 400 574 463
Fragments arranged by size:
574 463 400
AlwNI CAG_nnn'CTG
Cuts at: 0 388 1437
Size: 388 1049
ApaLI G'TGCA_C
Cuts at: 0 1253 1437
Size: 1253 184
ApoI r'AATT_y
Cuts at: 0 224 1437
Size: 224 1213
BamHI G'GATC_C
Cuts at: 0 245 317 1437
Size: 245 72 1120
Fragments arranged by size:
1120 245 72
BanI G'GyrC_C
Cuts at: 0 996 1437
Size: 996 441
BbvCI CC'TCA_GC
Cuts at: 0 1075 1437
Size: 1075 362
BclI T'GATC_A
Cuts at: 0 425 926 1418 1437
Size: 425 501 492 19
Fragments arranged by size:
501 492 425 19
BglI GCCn_nnn'nGGC
Cuts at: 0 919 1437
Size: 919 518
BglII A'GATC_T
Cuts at: 0 576 1437
Size: 576 861
Bme1580I G_kGCm'C
Cuts at: 0 890 1257 1437
Size: 890 367 180
Fragments arranged by size:
890 367 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 317 1014 1437
Size: 317 697 423
Fragments arranged by size:
697 423 317
Bpu10I CC'TnA_GC
Cuts at: 0 1075 1437
Size: 1075 362
BsaI GGTCTCn'nnnn_
Cuts at: 0 810 1437
Size: 810 627
BsaAI yAC'GTr
Cuts at: 0 278 1437
Size: 278 1159
BsaBI GATnn'nnATC
Cuts at: 0 269 964 1437
Size: 269 695 473
Fragments arranged by size:
695 473 269
BsaWI w'CCGG_w
Cuts at: 0 793 1437
Size: 793 644
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 788 818 1437
Size: 788 30 619
Fragments arranged by size:
788 619 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 717 721 755 1437
Size: 717 4 34 682
Fragments arranged by size:
717 682 34 4
BseYI C'CCAG_C
Cuts at: 0 783 1437
Size: 783 654
BsiEI CG_ry'CG
Cuts at: 0 994 1437
Size: 994 443
BsiHKAI G_wGCw'C
Cuts at: 0 1257 1437
Size: 1257 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 64 1437
Size: 64 1373
Bsp1286I G_dGCh'C
Cuts at: 0 890 1257 1437
Size: 890 367 180
Fragments arranged by size:
890 367 180
BspEI T'CCGG_A
Cuts at: 0 793 1437
Size: 793 644
BsrBI CCG'CTC
Cuts at: 0 1098 1432 1437
Size: 1098 334 5
Fragments arranged by size:
1098 334 5
BsrFI r'CCGG_y
Cuts at: 0 441 870 993 1437
Size: 441 429 123 444
Fragments arranged by size:
444 441 429 123
BssSI C'ACGA_G
Cuts at: 0 331 1437
Size: 331 1106
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 976 1437
Size: 828 148 461
Fragments arranged by size:
828 461 148
BstYI r'GATC_y
Cuts at: 0 35 245 317 576 1437
Size: 35 210 72 259 861
Fragments arranged by size:
861 259 210 72 35
Bsu36I CC'TnA_GG
Cuts at: 0 1232 1437
Size: 1232 205
BtgI C'CryG_G
Cuts at: 0 615 1437
Size: 615 822
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1275 1437
Size: 1275 162
BtsI GCAGTG_nn'
Cuts at: 0 508 938 1437
Size: 508 430 499
Fragments arranged by size:
508 499 430
EaeI y'GGCC_r
Cuts at: 0 443 830 991 1437
Size: 443 387 161 446
Fragments arranged by size:
446 443 387 161
EagI C'GGCC_G
Cuts at: 0 991 1437
Size: 991 446
EarI CTCTTCn'nnn_
Cuts at: 0 140 694 1437
Size: 140 554 743
Fragments arranged by size:
743 554 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 238 1437
Size: 238 1199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 718 1437
Size: 365 353 719
Fragments arranged by size:
719 365 353
EcoO109I rG'GnC_Cy
Cuts at: 0 707 1437
Size: 707 730
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 762 788 794 806 820 838
Size: 361 32 369 26 6 12 14 18
Cuts at: 838 1437
Size: 599
Fragments arranged by size:
599 369 361 32 26 18 14 12
6
HincII GTy'rAC
Cuts at: 0 726 947 1437
Size: 726 221 490
Fragments arranged by size:
726 490 221
MscI TGG'CCA
Cuts at: 0 832 1437
Size: 832 605
MslI CAynn'nnrTG
Cuts at: 0 400 955 958 974 1396 1437
Size: 400 555 3 16 422 41
Fragments arranged by size:
555 422 400 41 16 3
MspA1I CmG'CkG
Cuts at: 0 572 628 1437
Size: 572 56 809
Fragments arranged by size:
809 572 56
NaeI GCC'GGC
Cuts at: 0 872 1437
Size: 872 565
NcoI C'CATG_G
Cuts at: 0 615 1437
Size: 615 822
NgoMIV G'CCGG_C
Cuts at: 0 870 1437
Size: 870 567
NspI r_CATG'y
Cuts at: 0 624 1035 1437
Size: 624 411 402
Fragments arranged by size:
624 411 402
PasI CC'CwG_GG
Cuts at: 0 810 1437
Size: 810 627
PciI A'CATG_T
Cuts at: 0 1031 1437
Size: 1031 406
PfoI T'CCnGG_A
Cuts at: 0 523 1437
Size: 523 914
PmlI CAC'GTG
Cuts at: 0 278 1437
Size: 278 1159
PpuMI rG'GwC_Cy
Cuts at: 0 707 1437
Size: 707 730
PstI C_TGCA'G
Cuts at: 0 69 182 1437
Size: 69 113 1255
Fragments arranged by size:
1255 113 69
SapI GCTCTTCn'nnn_
Cuts at: 0 140 1437
Size: 140 1297
SfcI C'TryA_G
Cuts at: 0 65 178 1437
Size: 65 113 1259
Fragments arranged by size:
1259 113 65
SphI G_CATG'C
Cuts at: 0 624 1437
Size: 624 813
SspI AAT'ATT
Cuts at: 0 1281 1437
Size: 1281 156
StyI C'CwwG_G
Cuts at: 0 563 615 1328 1437
Size: 563 52 713 109
Fragments arranged by size:
713 563 109 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 1051 1382 1437
Size: 299 752 331 55
Fragments arranged by size:
752 331 299 55
TatI w'GTAC_w
Cuts at: 0 1259 1286 1437
Size: 1259 27 151
Fragments arranged by size:
1259 151 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 309 341 1437
Size: 309 32 1096
Fragments arranged by size:
1096 309 32
Tth111I GACn'n_nGTC
Cuts at: 0 349 1437
Size: 349 1088
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI ApoI BamHI BanI
BbvCI BclI BglI BglII Bme1580I BmrI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BsrBI BsrFI BssSI BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoO109I Hin4I HincII MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PfoI PmlI PpuMI
PstI SapI SfcI SphI SspI StyI TaqII TatI
TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BbsI BcgI BciVI BfrBI
BlpI BmgBI BmtI BplI BpmI BpuEI BsaHI BsgI
BsiWI BsmI BspHI BspMI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI PacI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI StuI SwaI XbaI XcmI XhoI XmaI
XmnI ZraI