(Linear) (Six-base) MAPSORT of: KIBB7184.seq  Check: 2043  from: 1  to: 663



KIBB7184.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     429     663 
   Size:        286     143     234

  Fragments arranged by size: 

                 286     234     143
 
BanI G'GyrC_C

Cuts at:      0     112     663 
   Size:        112     551
 
BanII G_rGCy'C

Cuts at:      0     356     663 
   Size:        356     307
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     435     663 
   Size:        435     228
 
Bme1580I G_kGCm'C

Cuts at:      0     493     663 
   Size:        493     170
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     663 
   Size:        238     425
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     663 
   Size:        340     323
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     368     398     663 
   Size:        368      30     265

  Fragments arranged by size: 

                 368     265      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274     663 
   Size:        274     389
 
Bsp1286I G_dGCh'C

Cuts at:      0     356     493     663 
   Size:        356     137     170

  Fragments arranged by size: 

                 356     170     137
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     120     663 
   Size:        120     543
 
EaeI y'GGCC_r

Cuts at:      0     206     663 
   Size:        206     457
 
EarI CTCTTCn'nnn_

Cuts at:      0     199     663 
   Size:        199     464
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     286     429     663 
   Size:        238      48     143     234

  Fragments arranged by size: 

                 238     234     143      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     513     565     663 
   Size:        513      52      98

  Fragments arranged by size: 

                 513      98      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     361     663 
   Size:        361     302
 
HincII GTy'rAC

Cuts at:      0     201     663 
   Size:        201     462
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     663 
   Size:        520     143
 
MscI TGG'CCA

Cuts at:      0     208     663 
   Size:        208     455
 
PfoI T'CCnGG_A

Cuts at:      0     254     663 
   Size:        254     409
 
PpuMI rG'GwC_Cy

Cuts at:      0     513     663 
   Size:        513     150
 
SanDI GG'GwC_CC

Cuts at:      0     513     663 
   Size:        513     150
 
SmlI C'TyrA_G

Cuts at:      0     355     663 
   Size:        355     308
 
XmnI GAAnn'nnTTC

Cuts at:      0     442     649     663 
   Size:        442     207      14

  Fragments arranged by size: 

                 442     207      14
 

 Enzymes that do cut:

     AcuI     BanI    BanII     BbsI Bme1580I     BpmI    BpuEI    BsaXI
    BseRI Bsp1286I    BtgZI     EaeI     EarI  Eco57MI EcoO109I     FalI
   HincII     MmeI     MscI     PfoI    PpuMI    SanDI     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI