Restriction map for
pF1KB7185
(Linear) (Six-base) MAPSORT of: KIBB7185.seq Check: 8815 from: 1 to: 1332
KIBB7185.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 325 1332
Size: 325 1007
AccI GT'mk_AC
Cuts at: 0 756 1332
Size: 756 576
Acc65I G'GTAC_C
Cuts at: 0 122 1332
Size: 122 1210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1332
Size: 1028 304
AfeI AGC'GCT
Cuts at: 0 637 861 1332
Size: 637 224 471
Fragments arranged by size:
637 471 224
AleI CACnn'nnGTG
Cuts at: 0 705 1332
Size: 705 627
ApaI G_GGCC'C
Cuts at: 0 507 1332
Size: 507 825
ApoI r'AATT_y
Cuts at: 0 112 650 1108 1120 1332
Size: 112 538 458 12 212
Fragments arranged by size:
538 458 212 112 12
AvaI C'yCGr_G
Cuts at: 0 824 1332
Size: 824 508
BanI G'GyrC_C
Cuts at: 0 7 122 699 1332
Size: 7 115 577 633
Fragments arranged by size:
633 577 115 7
BanII G_rGCy'C
Cuts at: 0 80 507 1332
Size: 80 427 825
Fragments arranged by size:
825 427 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 1016 1332
Size: 1016 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 721 755 1332
Size: 721 34 577
Fragments arranged by size:
721 577 34
BglI GCCn_nnn'nGGC
Cuts at: 0 828 1332
Size: 828 504
BlpI GC'TnA_GC
Cuts at: 0 597 1332
Size: 597 735
Bme1580I G_kGCm'C
Cuts at: 0 507 1332
Size: 507 825
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1332
Size: 56 1276
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 1332
Size: 346 986
BseYI C'CCAG_C
Cuts at: 0 638 1296 1332
Size: 638 658 36
Fragments arranged by size:
658 638 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 597 1332
Size: 127 470 735
Fragments arranged by size:
735 470 127
BsiHKAI G_wGCw'C
Cuts at: 0 305 493 1277 1332
Size: 305 188 784 55
Fragments arranged by size:
784 305 188 55
Bsp1286I G_dGCh'C
Cuts at: 0 80 305 493 507 1277 1332
Size: 80 225 188 14 770 55
Fragments arranged by size:
770 225 188 80 55 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 325 1332
Size: 149 176 1007
Fragments arranged by size:
1007 176 149
BssSI C'ACGA_G
Cuts at: 0 304 1332
Size: 304 1028
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1272 1332
Size: 1272 60
BstEII G'GTnAC_C
Cuts at: 0 261 1332
Size: 261 1071
BstXI CCAn_nnnn'nTGG
Cuts at: 0 570 1332
Size: 570 762
BstYI r'GATC_y
Cuts at: 0 761 884 1332
Size: 761 123 448
Fragments arranged by size:
761 448 123
Bsu36I CC'TnA_GG
Cuts at: 0 557 1332
Size: 557 775
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1147 1332
Size: 1147 185
BtsI GCAGTG_nn'
Cuts at: 0 239 1332
Size: 239 1093
DraI TTT'AAA
Cuts at: 0 86 1125 1332
Size: 86 1039 207
Fragments arranged by size:
1039 207 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1332
Size: 1028 304
EcoO109I rG'GnC_Cy
Cuts at: 0 165 1332
Size: 165 1167
EcoRI G'AATT_C
Cuts at: 0 112 1332
Size: 112 1220
EcoRV GAT'ATC
Cuts at: 0 345 1332
Size: 345 987
HaeII r_GCGC'y
Cuts at: 0 639 863 1332
Size: 639 224 469
Fragments arranged by size:
639 469 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 378 410 1234 1266 1332
Size: 378 32 824 32 66
Fragments arranged by size:
824 378 66 32 32
HincII GTy'rAC
Cuts at: 0 757 1332
Size: 757 575
KpnI G_GTAC'C
Cuts at: 0 126 1332
Size: 126 1206
MslI CAynn'nnrTG
Cuts at: 0 187 547 568 705 1332
Size: 187 360 21 137 627
Fragments arranged by size:
627 360 187 137 21
PfoI T'CCnGG_A
Cuts at: 0 850 1332
Size: 850 482
PpuMI rG'GwC_Cy
Cuts at: 0 165 1332
Size: 165 1167
PsiI TTA'TAA
Cuts at: 0 547 1091 1332
Size: 547 544 241
Fragments arranged by size:
547 544 241
PspOMI G'GGCC_C
Cuts at: 0 503 1332
Size: 503 829
PspXI vC'TCGA_Gb
Cuts at: 0 824 1332
Size: 824 508
PstI C_TGCA'G
Cuts at: 0 809 1332
Size: 809 523
SalI G'TCGA_C
Cuts at: 0 755 1332
Size: 755 577
SexAI A'CCwGG_T
Cuts at: 0 355 1332
Size: 355 977
SfcI C'TryA_G
Cuts at: 0 397 779 805 1332
Size: 397 382 26 527
Fragments arranged by size:
527 397 382 26
SmlI C'TyrA_G
Cuts at: 0 71 824 1332
Size: 71 753 508
Fragments arranged by size:
753 508 71
SspI AAT'ATT
Cuts at: 0 737 1332
Size: 737 595
StyI C'CwwG_G
Cuts at: 0 446 1149 1332
Size: 446 703 183
Fragments arranged by size:
703 446 183
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 306 338 1332
Size: 306 32 994
Fragments arranged by size:
994 306 32
Tth111I GACn'n_nGTC
Cuts at: 0 405 1332
Size: 405 927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 1325 1332
Size: 336 989 7
Fragments arranged by size:
989 336 7
XhoI C'TCGA_G
Cuts at: 0 824 1332
Size: 824 508
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI ApaI ApoI
AvaI BanI BanII BbsI BcgI BglI BlpI Bme1580I
BpuEI BsaI BseYI BsgI BsiHKAI Bsp1286I BspMI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI
Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII KpnI
MslI PfoI PpuMI PsiI PspOMI PspXI PstI SalI
SexAI SfcI SmlI SspI StyI TstI Tth111I XcmI
XhoI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstZ17I BtgI
ClaI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI FalI FseI FspI FspAI HindIII HpaI
KasI MfeI MluI MmeI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsrI PvuI PvuII RsrII SacI SacII SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TatI XbaI
XmaI XmnI ZraI