(Linear) (Six-base) MAPSORT of: KIBB7190.seq  Check: 3861  from: 1  to: 408



KIBB7190.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0      31     172     408 
   Size:         31     141     236

  Fragments arranged by size: 

                 236     141      31
 
AfeI AGC'GCT

Cuts at:      0      87     408 
   Size:         87     321
 
AlwNI CAG_nnn'CTG

Cuts at:      0      97     210     408 
   Size:         97     113     198

  Fragments arranged by size: 

                 198     113      97
 
BanI G'GyrC_C

Cuts at:      0      45     133     408 
   Size:         45      88     275

  Fragments arranged by size: 

                 275      88      45
 
BanII G_rGCy'C

Cuts at:      0     262     408 
   Size:        262     146
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323     408 
   Size:        323      85
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       4      38     162     196     408 
   Size:          4      34     124      34     212

  Fragments arranged by size: 

                 212     124      34      34       4
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     400     408 
   Size:        400       8
 
BglI GCCn_nnn'nGGC

Cuts at:      0      70     408 
   Size:         70     338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     408 
   Size:         74     334
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     313     408 
   Size:        313      95
 
BsiHKAI G_wGCw'C

Cuts at:      0     262     408 
   Size:        262     146
 
Bsp1286I G_dGCh'C

Cuts at:      0     262     408 
   Size:        262     146
 
BsrFI r'CCGG_y

Cuts at:      0      34     408 
   Size:         34     374
 
BssHII G'CGCG_C

Cuts at:      0      76     408 
   Size:         76     332
 
BstYI r'GATC_y

Cuts at:      0     152     408 
   Size:        152     256
 
Bsu36I CC'TnA_GG

Cuts at:      0     365     408 
   Size:        365      43
 
BtgI C'CryG_G

Cuts at:      0      49     137     393     408 
   Size:         49      88     256      15

  Fragments arranged by size: 

                 256      88      49      15
 
EaeI y'GGCC_r

Cuts at:      0      62     408 
   Size:         62     346
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     408 
   Size:        145     263
 
EcoICRI GAG'CTC

Cuts at:      0     260     408 
   Size:        260     148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     408 
   Size:         74     334
 
HaeII r_GCGC'y

Cuts at:      0      89     210     277     408 
   Size:         89     121      67     131

  Fragments arranged by size: 

                 131     121      89      67
 
HincII GTy'rAC

Cuts at:      0     173     408 
   Size:        173     235
 
MscI TGG'CCA

Cuts at:      0      64     408 
   Size:         64     344
 
MslI CAynn'nnrTG

Cuts at:      0     358     408 
   Size:        358      50
 
MspA1I CmG'CkG

Cuts at:      0      51      60     408 
   Size:         51       9     348

  Fragments arranged by size: 

                 348      51       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      97     408 
   Size:         97     311
 
PvuII CAG'CTG

Cuts at:      0      60     408 
   Size:         60     348
 
SacI G_AGCT'C

Cuts at:      0     262     408 
   Size:        262     146
 
SacII CC_GC'GG

Cuts at:      0      52     408 
   Size:         52     356
 
SalI G'TCGA_C

Cuts at:      0     171     408 
   Size:        171     237
 
SfcI C'TryA_G

Cuts at:      0     123     408 
   Size:        123     285
 
StuI AGG'CCT

Cuts at:      0     295     408 
   Size:        295     113
 
StyI C'CwwG_G

Cuts at:      0      68     408 
   Size:         68     340
 

 Enzymes that do cut:

     AccI     AfeI    AlwNI     BanI    BanII     BbsI     BcgI    BciVI
     BglI     BpmI     BsaI  BsiHKAI Bsp1286I    BsrFI   BssHII    BstYI
   Bsu36I     BtgI     EaeI     EciI  EcoICRI  Eco57MI    HaeII   HincII
     MscI     MslI   MspA1I    PflMI    PvuII     SacI    SacII     SalI
     SfcI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI