(Linear) (Six-base) MAPSORT of: KIBB7209.seq  Check: 3037  from: 1  to: 603



KIBB7209.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     548     580     603 
   Size:        548      32      23

  Fragments arranged by size: 

                 548      32      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     417     603 
   Size:        417     186
 
BanII G_rGCy'C

Cuts at:      0     343     356     603 
   Size:        343      13     247

  Fragments arranged by size: 

                 343     247      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     107     603 
   Size:        107     496
 
BclI T'GATC_A

Cuts at:      0     170     603 
   Size:        170     433
 
BfrBI ATG'CAT

Cuts at:      0     119     603 
   Size:        119     484
 
BlpI GC'TnA_GC

Cuts at:      0     518     603 
   Size:        518      85
 
BmgBI CAC'GTC

Cuts at:      0      80     603 
   Size:         80     523
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     282     603 
   Size:        282     321
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     603 
   Size:        319     284
 
BsaBI GATnn'nnATC

Cuts at:      0     175     603 
   Size:        175     428
 
BsaWI w'CCGG_w

Cuts at:      0      74     603 
   Size:         74     529
 
BseYI C'CCAG_C

Cuts at:      0     451     603 
   Size:        451     152
 
BsiHKAI G_wGCw'C

Cuts at:      0     343     603 
   Size:        343     260
 
Bsp1286I G_dGCh'C

Cuts at:      0     343     356     603 
   Size:        343      13     247

  Fragments arranged by size: 

                 343     247      13
 
BspEI T'CCGG_A

Cuts at:      0      74     603 
   Size:         74     529
 
BtgI C'CryG_G

Cuts at:      0     479     603 
   Size:        479     124
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     481     603 
   Size:        481     122
 
EcoICRI GAG'CTC

Cuts at:      0     341     603 
   Size:        341     262
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     603 
   Size:        319     284
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     110     603 
   Size:        110     493
 
EcoO109I rG'GnC_Cy

Cuts at:      0     365     414     603 
   Size:        365      49     189

  Fragments arranged by size: 

                 365     189      49
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     216     603 
   Size:        216     387
 
MslI CAynn'nnrTG

Cuts at:      0     159     603 
   Size:        159     444
 
MspA1I CmG'CkG

Cuts at:      0     239     603 
   Size:        239     364
 
NcoI C'CATG_G

Cuts at:      0     479     603 
   Size:        479     124
 
NdeI CA'TA_TG

Cuts at:      0      11     603 
   Size:         11     592
 
NsiI A_TGCA'T

Cuts at:      0     121     603 
   Size:        121     482
 
NspI r_CATG'y

Cuts at:      0     534     603 
   Size:        534      69
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      41      73     603 
   Size:         41      32     530

  Fragments arranged by size: 

                 530      41      32
 
SacI G_AGCT'C

Cuts at:      0     343     603 
   Size:        343     260
 
ScaI AGT'ACT

Cuts at:      0     274     603 
   Size:        274     329
 
SfcI C'TryA_G

Cuts at:      0     110     603 
   Size:        110     493
 
StyI C'CwwG_G

Cuts at:      0     479     603 
   Size:        479     124
 
TatI w'GTAC_w

Cuts at:      0     224     272     533     603 
   Size:        224      48     261      70

  Fragments arranged by size: 

                 261     224      70      48
 

 Enzymes that do cut:

     AloI    AlwNI    BanII     BbsI     BclI    BfrBI     BlpI    BmgBI
     BmrI     BpmI    BsaBI    BsaWI    BseYI  BsiHKAI Bsp1286I    BspEI
     BtgI     DrdI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     MslI
   MspA1I     NcoI     NdeI     NsiI     NspI     PsrI     SacI     ScaI
     SfcI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BglI    BglII Bme1580I     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EaeI     EagI     EarI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI