(Linear) (Six-base) MAPSORT of: KIBB7210.seq Check: 7177 from: 1 to: 363 KIBB7210.orf With 182 enzymes: SgfI * March 3, 2008 10:59 .. AccI GT'mk_AC Cuts at: 0 273 363 Size: 273 90 AflII C'TTAA_G Cuts at: 0 193 363 Size: 193 170 ApoI r'AATT_y Cuts at: 0 330 337 363 Size: 330 7 26 Fragments arranged by size: 330 26 7 BmtI G_CTAG'C Cuts at: 0 62 363 Size: 62 301 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 138 363 Size: 138 225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 363 Size: 133 230 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 28 363 Size: 28 335 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 48 363 Size: 48 315 BstZ17I GTA'TAC Cuts at: 0 274 363 Size: 274 89 DraI TTT'AAA Cuts at: 0 336 363 Size: 336 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 363 Size: 133 230 NheI G'CTAG_C Cuts at: 0 58 363 Size: 58 305 SmlI C'TyrA_G Cuts at: 0 193 363 Size: 193 170 Enzymes that do cut: AccI AflII ApoI BmtI BplI BpmI BsaXI BspMI BstZ17I DraI Eco57MI NheI SmlI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI