(Linear) (Six-base) MAPSORT of: KIBB7212.seq  Check: 2680  from: 1  to: 309



KIBB7212.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     263     309 
   Size:        263      46
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     309 
   Size:        196     113
 
BanI G'GyrC_C

Cuts at:      0     107     309 
   Size:        107     202
 
BbeI G_GCGC'C

Cuts at:      0     111     309 
   Size:        111     198
 
BglI GCCn_nnn'nGGC

Cuts at:      0     125     309 
   Size:        125     184
 
BlpI GC'TnA_GC

Cuts at:      0      47     309 
   Size:         47     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     309 
   Size:        256      53
 
BsaHI Gr'CG_yC

Cuts at:      0     108     206     309 
   Size:        108      98     103

  Fragments arranged by size: 

                 108     103      98
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     309 
   Size:        172     137
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     309 
   Size:        227      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     309 
   Size:        227      82
 
BsrFI r'CCGG_y

Cuts at:      0      96     309 
   Size:         96     213
 
BstEII G'GTnAC_C

Cuts at:      0     243     309 
   Size:        243      66
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     258     309 
   Size:        258      51
 
BtgI C'CryG_G

Cuts at:      0       9     120     251     309 
   Size:          9     111     131      58

  Fragments arranged by size: 

                 131     111      58       9
 
EaeI y'GGCC_r

Cuts at:      0       6      98     309 
   Size:          6      92     211

  Fragments arranged by size: 

                 211      92       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     208     309 
   Size:        196      12     101

  Fragments arranged by size: 

                 196     101      12
 
HaeII r_GCGC'y

Cuts at:      0      47      54     111     279     309 
   Size:         47       7      57     168      30

  Fragments arranged by size: 

                 168      57      47      30       7
 
KasI G'GCGC_C

Cuts at:      0     107     309 
   Size:        107     202
 
MslI CAynn'nnrTG

Cuts at:      0     250     256     309 
   Size:        250       6      53

  Fragments arranged by size: 

                 250      53       6
 
MspA1I CmG'CkG

Cuts at:      0      11     122     309 
   Size:         11     111     187

  Fragments arranged by size: 

                 187     111      11
 
NaeI GCC'GGC

Cuts at:      0      98     309 
   Size:         98     211
 
NarI GG'CG_CC

Cuts at:      0     108     309 
   Size:        108     201
 
NcoI C'CATG_G

Cuts at:      0     251     309 
   Size:        251      58
 
NgoMIV G'CCGG_C

Cuts at:      0      96     309 
   Size:         96     213
 
PfoI T'CCnGG_A

Cuts at:      0     185     200     309 
   Size:        185      15     109

  Fragments arranged by size: 

                 185     109      15
 
SacII CC_GC'GG

Cuts at:      0      12     123     309 
   Size:         12     111     186

  Fragments arranged by size: 

                 186     111      12
 
ScaI AGT'ACT

Cuts at:      0      65     309 
   Size:         65     244
 
SfoI GGC'GCC

Cuts at:      0     109     309 
   Size:        109     200
 
SmlI C'TyrA_G

Cuts at:      0     271     309 
   Size:        271      38
 
StyI C'CwwG_G

Cuts at:      0     251     309 
   Size:        251      58
 
TatI w'GTAC_w

Cuts at:      0      63     309 
   Size:         63     246
 
XmnI GAAnn'nnTTC

Cuts at:      0     181     309 
   Size:        181     128
 

 Enzymes that do cut:

     AccI     AcuI     BanI     BbeI     BglI     BlpI     BpmI    BpuEI
    BsaHI    BseRI  BsiHKAI Bsp1286I    BsrFI   BstEII    BstXI     BtgI
     EaeI  Eco57MI    HaeII     KasI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     PfoI    SacII     ScaI     SfoI     SmlI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI