Restriction map for
pF1KB7214
(Linear) (Six-base) MAPSORT of: KIBB7214.seq Check: 633 from: 1 to: 879
KIBB7214.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 270 879
Size: 270 609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 182 640 879
Size: 34 148 458 239
Fragments arranged by size:
458 239 148 34
AleI CACnn'nnGTG
Cuts at: 0 213 340 427 879
Size: 213 127 87 452
Fragments arranged by size:
452 213 127 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 375 879
Size: 375 504
AlwNI CAG_nnn'CTG
Cuts at: 0 160 599 879
Size: 160 439 280
Fragments arranged by size:
439 280 160
ApaI G_GGCC'C
Cuts at: 0 172 879
Size: 172 707
ApoI r'AATT_y
Cuts at: 0 528 570 650 879
Size: 528 42 80 229
Fragments arranged by size:
528 229 80 42
AvaI C'yCGr_G
Cuts at: 0 20 61 879
Size: 20 41 818
Fragments arranged by size:
818 41 20
BanI G'GyrC_C
Cuts at: 0 87 319 879
Size: 87 232 560
Fragments arranged by size:
560 232 87
BanII G_rGCy'C
Cuts at: 0 148 172 879
Size: 148 24 707
Fragments arranged by size:
707 148 24
BbeI G_GCGC'C
Cuts at: 0 91 879
Size: 91 788
Bme1580I G_kGCm'C
Cuts at: 0 172 879
Size: 172 707
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 879
Size: 81 798
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 167 879
Size: 167 712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 493 879
Size: 137 356 386
Fragments arranged by size:
386 356 137
BsaI GGTCTCn'nnnn_
Cuts at: 0 441 879
Size: 441 438
BsaAI yAC'GTr
Cuts at: 0 338 879
Size: 338 541
BsaHI Gr'CG_yC
Cuts at: 0 88 879
Size: 88 791
BsaWI w'CCGG_w
Cuts at: 0 607 879
Size: 607 272
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 133 260 879
Size: 100 33 127 619
Fragments arranged by size:
619 127 100 33
BseYI C'CCAG_C
Cuts at: 0 599 857 879
Size: 599 258 22
Fragments arranged by size:
599 258 22
Bsp1286I G_dGCh'C
Cuts at: 0 148 172 879
Size: 148 24 707
Fragments arranged by size:
707 148 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 270 879
Size: 270 609
BstBI TT'CG_AA
Cuts at: 0 489 879
Size: 489 390
BstEII G'GTnAC_C
Cuts at: 0 327 603 879
Size: 327 276 276
Fragments arranged by size:
327 276 276
Bsu36I CC'TnA_GG
Cuts at: 0 563 879
Size: 563 316
BtsI GCAGTG_nn'
Cuts at: 0 208 357 879
Size: 208 149 522
Fragments arranged by size:
522 208 149
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 413 448 879
Size: 413 35 431
Fragments arranged by size:
431 413 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 835 879
Size: 835 44
EaeI y'GGCC_r
Cuts at: 0 219 879
Size: 219 660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 137 182 493 640 879
Size: 34 103 45 311 147 239
Fragments arranged by size:
311 239 147 103 45 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 879
Size: 115 764
EcoO109I rG'GnC_Cy
Cuts at: 0 24 157 169 879
Size: 24 133 12 710
Fragments arranged by size:
710 133 24 12
HaeII r_GCGC'y
Cuts at: 0 91 879
Size: 91 788
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 384 416 440 472 879
Size: 384 32 24 32 407
Fragments arranged by size:
407 384 32 32 24
KasI G'GCGC_C
Cuts at: 0 87 879
Size: 87 792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 879
Size: 540 339
MslI CAynn'nnrTG
Cuts at: 0 213 340 383 427 879
Size: 213 127 43 44 452
Fragments arranged by size:
452 213 127 44 43
NarI GG'CG_CC
Cuts at: 0 88 879
Size: 88 791
PflMI CCAn_nnn'nTGG
Cuts at: 0 599 879
Size: 599 280
PfoI T'CCnGG_A
Cuts at: 0 153 879
Size: 153 726
PmlI CAC'GTG
Cuts at: 0 338 879
Size: 338 541
PpuMI rG'GwC_Cy
Cuts at: 0 157 879
Size: 157 722
PspOMI G'GGCC_C
Cuts at: 0 168 879
Size: 168 711
SexAI A'CCwGG_T
Cuts at: 0 592 879
Size: 592 287
SfoI GGC'GCC
Cuts at: 0 89 879
Size: 89 790
SmaI CCC'GGG
Cuts at: 0 22 63 879
Size: 22 41 816
Fragments arranged by size:
816 41 22
SrfI GCCC'GGGC
Cuts at: 0 63 879
Size: 63 816
StuI AGG'CCT
Cuts at: 0 715 879
Size: 715 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 330 591 879
Size: 330 261 288
Fragments arranged by size:
330 288 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 253 285 349 381 741 773 879
Size: 253 32 64 32 360 32 106
Fragments arranged by size:
360 253 106 64 32 32 32
XmaI C'CCGG_G
Cuts at: 0 20 61 879
Size: 20 41 818
Fragments arranged by size:
818 41 20
XmnI GAAnn'nnTTC
Cuts at: 0 653 780 879
Size: 653 127 99
Fragments arranged by size:
653 127 99
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbeI Bme1580I BmrI BplI BpmI BsaI
BsaAI BsaHI BsaWI BseRI BseYI Bsp1286I BspMI BstBI
BstEII Bsu36I BtsI CspCI DrdI EaeI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MmeI MslI NarI PflMI
PfoI PmlI PpuMI PspOMI SexAI SfoI SmaI SrfI
StuI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaBI BsaXI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI
BstYI BstZ17I BtgI BtgZI ClaI DraI DraIII EagI
EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StyI
SwaI TatI Tth111I XbaI XcmI XhoI ZraI