Restriction map for
pF1KB7222
(Linear) (Six-base) MAPSORT of: KIBB7222.seq Check: 8418 from: 1 to: 1365
KIBB7222.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 882 1365
Size: 882 483
AatII G_ACGT'C
Cuts at: 0 1157 1365
Size: 1157 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 691 712 872 1365
Size: 528 163 21 160 493
Fragments arranged by size:
528 493 163 160 21
AflIII A'CryG_T
Cuts at: 0 416 1184 1365
Size: 416 768 181
Fragments arranged by size:
768 416 181
AhdI GACnn_n'nnGTC
Cuts at: 0 432 955 1365
Size: 432 523 410
Fragments arranged by size:
523 432 410
AleI CACnn'nnGTG
Cuts at: 0 79 1081 1365
Size: 79 1002 284
Fragments arranged by size:
1002 284 79
AlwNI CAG_nnn'CTG
Cuts at: 0 1146 1365
Size: 1146 219
ApaI G_GGCC'C
Cuts at: 0 1164 1365
Size: 1164 201
ApaLI G'TGCA_C
Cuts at: 0 1238 1365
Size: 1238 127
BamHI G'GATC_C
Cuts at: 0 614 1365
Size: 614 751
BanI G'GyrC_C
Cuts at: 0 809 1365
Size: 809 556
BanII G_rGCy'C
Cuts at: 0 52 73 461 1164 1365
Size: 52 21 388 703 201
Fragments arranged by size:
703 388 201 52 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 475 860 1270 1365
Size: 475 385 410 95
Fragments arranged by size:
475 410 385 95
BclI T'GATC_A
Cuts at: 0 1073 1365
Size: 1073 292
BglI GCCn_nnn'nGGC
Cuts at: 0 326 529 1096 1365
Size: 326 203 567 269
Fragments arranged by size:
567 326 269 203
BlpI GC'TnA_GC
Cuts at: 0 986 1365
Size: 986 379
Bme1580I G_kGCm'C
Cuts at: 0 424 1164 1242 1346 1365
Size: 424 740 78 104 19
Fragments arranged by size:
740 424 104 78 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 242 355 373 394 445 670 817
Size: 81 161 113 18 21 51 225 147
Cuts at: 817 988 1138 1189 1222 1365
Size: 171 150 51 33 143
Fragments arranged by size:
225 171 161 150 147 143 113 81
51 51 33 21 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 28 1365
Size: 1 27 1337
Fragments arranged by size:
1337 27 1
BsaI GGTCTCn'nnnn_
Cuts at: 0 44 289 1365
Size: 44 245 1076
Fragments arranged by size:
1076 245 44
BsaAI yAC'GTr
Cuts at: 0 1187 1365
Size: 1187 178
BsaHI Gr'CG_yC
Cuts at: 0 1154 1365
Size: 1154 211
BsaWI w'CCGG_w
Cuts at: 0 443 1365
Size: 443 922
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 793 823 1240 1270 1365
Size: 793 30 417 30 95
Fragments arranged by size:
793 417 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 691 721 814 892 1365
Size: 469 222 30 93 78 473
Fragments arranged by size:
473 469 222 93 78 30
BseYI C'CCAG_C
Cuts at: 0 1049 1091 1365
Size: 1049 42 274
Fragments arranged by size:
1049 274 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1325 1365
Size: 1325 40
BsiEI CG_ry'CG
Cuts at: 0 318 1365
Size: 318 1047
BsiHKAI G_wGCw'C
Cuts at: 0 461 1242 1365
Size: 461 781 123
Fragments arranged by size:
781 461 123
BsmI GAATG_Cn'
Cuts at: 0 498 1365
Size: 498 867
BsmBI CGTCTCn'nnnn_
Cuts at: 0 553 942 1021 1365
Size: 553 389 79 344
Fragments arranged by size:
553 389 344 79
Bsp1286I G_dGCh'C
Cuts at: 0 52 73 424 461 1164 1242 1346 1365
Size: 52 21 351 37 703 78 104 19
Fragments arranged by size:
703 351 104 78 52 37 21 19
BspEI T'CCGG_A
Cuts at: 0 443 1365
Size: 443 922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 396 882 1365
Size: 396 486 483
Fragments arranged by size:
486 483 396
BsrBI CCG'CTC
Cuts at: 0 1122 1365
Size: 1122 243
BsrDI GCAATG_nn'
Cuts at: 0 95 291 1365
Size: 95 196 1074
Fragments arranged by size:
1074 196 95
BsrFI r'CCGG_y
Cuts at: 0 22 325 1365
Size: 22 303 1040
Fragments arranged by size:
1040 303 22
BssSI C'ACGA_G
Cuts at: 0 77 1365
Size: 77 1288
BstAPI GCAn_nnn'nTGC
Cuts at: 0 107 1319 1365
Size: 107 1212 46
Fragments arranged by size:
1212 107 46
BstEII G'GTnAC_C
Cuts at: 0 739 1365
Size: 739 626
BstXI CCAn_nnnn'nTGG
Cuts at: 0 504 1365
Size: 504 861
BstYI r'GATC_y
Cuts at: 0 614 1103 1365
Size: 614 489 262
Fragments arranged by size:
614 489 262
BtsI GCAGTG_nn'
Cuts at: 0 721 1072 1365
Size: 721 351 293
Fragments arranged by size:
721 351 293
DrdI GACnn_nn'nnGTC
Cuts at: 0 567 1365
Size: 567 798
EaeI y'GGCC_r
Cuts at: 0 134 323 1097 1365
Size: 134 189 774 268
Fragments arranged by size:
774 268 189 134
EarI CTCTTCn'nnn_
Cuts at: 0 36 1365
Size: 36 1329
EcoICRI GAG'CTC
Cuts at: 0 459 1365
Size: 459 906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 242 355 373 394 445 528 670
Size: 81 161 113 18 21 51 83 142
Cuts at: 670 691 712 817 872 988 1138 1189 1222
Size: 21 21 105 55 116 150 51 33
Cuts at: 1222 1365
Size: 143
Fragments arranged by size:
161 150 143 142 116 113 105 83
81 55 51 51 33 21 21 21
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 221 337 673 1365
Size: 221 116 336 692
Fragments arranged by size:
692 336 221 116
EcoO109I rG'GnC_Cy
Cuts at: 0 408 1194 1365
Size: 408 786 171
Fragments arranged by size:
786 408 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 1365
Size: 42 1323
FspI TGC'GCA
Cuts at: 0 610 1365
Size: 610 755
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 941 973 1365
Size: 418 32 491 32 392
Fragments arranged by size:
491 418 392 32 32
HincII GTy'rAC
Cuts at: 0 780 960 1365
Size: 780 180 405
Fragments arranged by size:
780 405 180
HindIII A'AGCT_T
Cuts at: 0 112 1365
Size: 112 1253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1365
Size: 842 523
MscI TGG'CCA
Cuts at: 0 136 1365
Size: 136 1229
MslI CAynn'nnrTG
Cuts at: 0 79 1081 1339 1365
Size: 79 1002 258 26
Fragments arranged by size:
1002 258 79 26
MspA1I CmG'CkG
Cuts at: 0 194 351 582 1053 1095 1365
Size: 194 157 231 471 42 270
Fragments arranged by size:
471 270 231 194 157 42
NaeI GCC'GGC
Cuts at: 0 24 327 1365
Size: 24 303 1038
Fragments arranged by size:
1038 303 24
NgoMIV G'CCGG_C
Cuts at: 0 22 325 1365
Size: 22 303 1040
Fragments arranged by size:
1040 303 22
NspI r_CATG'y
Cuts at: 0 420 1365
Size: 420 945
PasI CC'CwG_GG
Cuts at: 0 1351 1365
Size: 1351 14
PciI A'CATG_T
Cuts at: 0 416 1365
Size: 416 949
PflMI CCAn_nnn'nTGG
Cuts at: 0 894 1365
Size: 894 471
PfoI T'CCnGG_A
Cuts at: 0 617 1365
Size: 617 748
PpuMI rG'GwC_Cy
Cuts at: 0 408 1365
Size: 408 957
PspOMI G'GGCC_C
Cuts at: 0 1160 1365
Size: 1160 205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1365
Size: 1120 32 213
Fragments arranged by size:
1120 213 32
PstI C_TGCA'G
Cuts at: 0 537 983 1324 1365
Size: 537 446 341 41
Fragments arranged by size:
537 446 341 41
PvuII CAG'CTG
Cuts at: 0 194 351 582 1053 1095 1365
Size: 194 157 231 471 42 270
Fragments arranged by size:
471 270 231 194 157 42
SacI G_AGCT'C
Cuts at: 0 461 1365
Size: 461 904
SexAI A'CCwGG_T
Cuts at: 0 1139 1365
Size: 1139 226
SfcI C'TryA_G
Cuts at: 0 180 240 533 979 1041 1320 1365
Size: 180 60 293 446 62 279 45
Fragments arranged by size:
446 293 279 180 62 60 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1096 1365
Size: 1096 269
SmlI C'TyrA_G
Cuts at: 0 16 43 1365
Size: 16 27 1322
Fragments arranged by size:
1322 27 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 883 1365
Size: 883 482
TatI w'GTAC_w
Cuts at: 0 92 1365
Size: 92 1273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 793 825 1365
Size: 793 32 540
Fragments arranged by size:
793 540 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 1365
Size: 504 861
ZraI GAC'GTC
Cuts at: 0 1155 1365
Size: 1155 210
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlwNI ApaI
ApaLI BamHI BanI BanII BbsI BclI BglI BlpI
Bme1580I BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI BtsI DrdI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI Hin4I HincII HindIII MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI
PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SacI
SexAI SfcI SfiI SmlI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AlfI AloI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI
BplI Bpu10I BsaBI BsiWI BspHI BsrGI BssHII BstBI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoRI EcoRV FseI FspAI HaeII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI Tth111I XbaI
XhoI XmaI XmnI